Anti-Tau (phospho S198) antibody [EPR2400] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S198) antibody [EPR2400] - BSA and Azide free (AB156622)

This data was developed using ab79540, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast cancer tissue sections labeling Tau with purified ab79540 at 1 : 1000 (0.423 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used. PBS instead of the primary antibody was used as the negative control. Postive staining on human breast cancer without alkaline phosphatase treatment (image A). No staining on human breast cancer with alkaline phosphatase treatment (image B). The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

• IP

Lab

Immunoprecipitation - Anti-Tau (phospho S198) antibody [EPR2400] - BSA and Azide free (AB156622)

This data was developed using ab79540, the same antibody clone in a different buffer formulation.
Purified ab79540 at 1/50 dilution (2μg) immunoprecipitating Tau in SH-SY5Y whole cell lysate.
Lane 1 (input) : SY-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate 10μg
Lane 2 (+) : ab79540 + SH-SY5Y whole cell lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab79540 in SH-SY5Y whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 50-70 kDa

All lanes:

Immunoprecipitation - Anti-Tau (phospho S198) antibody [EPR2400] (<a href='/en-us/products/primary-antibodies/tau-phospho-s198-antibody-epr2400-ab79540'>ab79540</a>)

Predicted band size: 78 kDa

false

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S198) antibody [EPR2400] - BSA and Azide free (AB156622)

This data was developed using ab79540, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebrum tissue sections labeling Tau with purified ab79540 at 1 : 1000 (0.423 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used. PBS instead of the primary antibody was used as the negative control. Postive staining on rat cerebrum without alkaline phosphatase treatment (image A). No staining on rat cerebrum with alkaline phosphatase treatment (image B). The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S198) antibody [EPR2400] - BSA and Azide free (AB156622)

This data was developed using ab79540, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebrum tissue sections labeling Tau with purified ab79540 at 1 : 1000 (0.423 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used. PBS instead of the primary antibody was used as the negative control. Postive staining on mouse cerebrum without alkaline phosphatase treatment (image A). No staining on mouse cerebrum with alkaline phosphatase treatment (image B). The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

• WB

Supplier Data

Western blot - Anti-Tau (phospho S198) antibody [EPR2400] - BSA and Azide free (AB156622)

This data was developed using ab79540, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Tau (phospho S198) antibody [EPR2400] (<a href='/en-us/products/primary-antibodies/tau-phospho-s198-antibody-epr2400-ab79540'>ab79540</a>) at 1/1000 dilution

Lane 1:

Untreated SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate at 15 µg

Lane 2:

SH-SY5Y treated with 1µM okadaic acid and 200nM calyculin a for 60 minutes, whole cell lysate at 15 µg

Lane 3:

SH-SY5Y treated with 1µM okadaic acid and 200nM calyculin a for 60 minutes whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 78 kDa

Observed band size: 50-70 kDa

false

• WB

Supplier Data

Western blot - Anti-Tau (phospho S198) antibody [EPR2400] - BSA and Azide free (AB156622)

This data was developed using ab79540, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Tau (phospho S198) antibody [EPR2400] (<a href='/en-us/products/primary-antibodies/tau-phospho-s198-antibody-epr2400-ab79540'>ab79540</a>) at 1/10000 dilution

Lane 1:

Mouse hippocampus lysate at 15 µg

Lane 2:

Mouse hippocampus lysate, the membrane treated with Alkaline Phosphatase for 1 hour at 15 µg

Lane 3:

Rat hippocampus lysate at 15 µg

Lane 4:

Rat hippocampus lysate, the membrane treated with Alkaline Phosphatase for 1 hour at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 78 kDa

Observed band size: 50-70 kDa

false