Rabbit Polyclonal TAU phospho S199 antibody. Suitable for WB, IHC-P and reacts with African green monkey, Mouse, Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human MAPT phospho S199.
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- Science advances 8:eabl88092022Alzheimer's disease: Ablating single master site abolishes tau hyperphosphorylation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKristie Stefanoska,Mehul Gajwani,Amanda R P Tan,Holly I Ahel,Prita R Asih,Alexander Volkerling,Anne Poljak,Arne IttnerPubMed 35857446
- Frontiers in molecular neuroscience 15:8523682022Hyperphosphorylated Human Tau Accumulates at the Synapse, Localizing on Synaptic Mitochondrial Outer Membranes and Disrupting Respiration in a Mouse Model of Tauopathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndrew J Trease,Joseph W George,Nashanthea J Roland,Eliezer Z Lichter,Katy Emanuel,Steven Totusek,Howard S Fox,Kelly L StauchPubMed 35359570
- Molecular neurodegeneration 16:782021CCR5 antagonist reduces HIV-induced amyloidogenesis, tau pathology, neurodegeneration, and blood-brain barrier alterations in HIV-infected hu-PBL-NSG mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBiju Bhargavan,Shawna M Woollard,Jo Ellyn McMillan,Georgette D KanmognePubMed 34809709
- Scientific reports 11:200752021Hypermethylation of Mest promoter causes aberrant Wnt signaling in patients with Alzheimer's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRenuka Prasad,Hwajin Jung,Anderson Tan,Yonghee Song,Sungho Moon,Mohammed R Shaker,Woong Sun,Junghee Lee,Hoon Ryu,Hyun Kook Lim,Eek-Hoon JhoPubMed 34625606
- Frontiers in neuroscience 14:5854762020Electroacupuncture Protects Cognition by Regulating Tau Phosphorylation and Glucose Metabolism via the AKT/GSK3β Signaling Pathway in Alzheimer's Disease Model Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnping Xu et. al.PubMed 33328854
- Experimental & molecular medicine 49:e3332017Transcriptome analyses of chronic traumatic encephalopathy show alterations in protein phosphatase expression associated with tauopathy.
- Journal of Alzheimer's disease : JAD 35:525-392013Natural cannabinoids improve dopamine neurotransmission and tau and amyloid pathology in a mouse model of tauopathy.Applications:IHC-PReactive species:MouseMaria J Casarejos,Juan Perucho,Ana Gomez,Maria P Muñoz,Marian Fernandez-Estevez,Onintza Sagredo,Javier Fernandez Ruiz,Manuel Guzman,Justo Garcia de Yebenes,Maria A MenaPubMed 23478312
- Neurobiology of disease 39:423-382010Trehalose ameliorates dopaminergic and tau pathology in parkin deleted/tau overexpressing mice through autophagy activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJose A Rodríguez-Navarro,Laura Rodríguez,María J Casarejos,Rosa M Solano,Ana Gómez,Juan Perucho,Ana María Cuervo,Justo García de Yébenes,María A MenaPubMed 20546895
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human MAPT phospho S199. The exact immunogen used to generate this antibody is proprietary information. Database link P10636
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Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Expected | Tested |
| Mouse | Expected | Tested |
| Rat | Predicted | Predicted |
| African green monkey | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species African green monkey | Dilution info 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/20 - 1/200 | Notes - |
Species Human | Dilution info 1/20 - 1/200 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species African green monkey | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
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Target data
Function
Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed:21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed:21985311, PubMed:32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
Additional Targets
MAPT phospho S199
Alternative names
MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau
Recommended products
Rabbit Polyclonal TAU phospho S199 antibody. Suitable for WB, IHC-P and reacts with African green monkey, Mouse, Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human MAPT phospho S199.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human MAPT phospho S199. The exact immunogen used to generate this antibody is proprietary information. Database link P10636
- Purification technique
- Affinity purification Immunogen
- Specificity
The specificity of this antibody refers to P10636-8.
- Concentration
- Purification notes
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated tau. The final product is generated by affinity chromatography using a tau-derived peptide that is phosphorylated at serine 199.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.
- Biological function summary
Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.
- Pathways
Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.
- Associated diseases and disorders
Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S199) antibody (ab4749)
Paraffin embedded human brain tissue (right) stained for Tau using ab4749 at 1/100 dilution in immunohistochemical analysis. Negative control without primary antibody (left).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S199) antibody (ab4749)
Paraffin embedded mouse brain tissue (right) stained for Tau using ab4749 at 1/100 dilution in immunohistochemical analysis. Negative control without primary antibody (left).
Western blot - Anti-Tau (phospho S199) antibody (ab4749)
Cell extracts from African green monkey kidney (CV-1) cells, stably expressing human four repeat tau and a protein phosphatase inhibitor, were resolved by SDS-PAGE on a 10% Tris-glycine gel. The proteins were transferred to nitrocellulose. Membranes were incubated with 0.50 μg/mL anti-phospho tau [pS199] (ab4749), following prior incubation in the absence (a) or presence of the peptide immunogen (b), or the nonphosphopeptide corresponding to the tau phosphopeptide (c). Cell extracts from African green monkey kidney (CV-1) cells, stably expressing human four repeat tau and a protein phosphatase inhibitor, were resolved by SDS-PAGE on a 10% Tris-glycine gel. The proteins were transferred to nitrocellulose. Membranes were incubated with 0.50 µg/mL anti-phospho tau [pS199] (ab4749), following prior incubation in the absence (a) or presence of the peptide immunogen (b), or the nonphosphopeptide corresponding to the tau phosphopeptide (c).
All lanes: Western blot - Anti-Tau (phospho S199) antibody (ab4749)
Predicted band size: 78 kDa
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