Anti-Tau (phospho S202) antibody [EPR2402]

• WB

Supplier Data

Western blot - Anti-Tau (phospho S202) antibody [EPR2402] (AB108387)

The molecular weight observed is consistent with what has been described in the literature (PMID : 28382304, 32692785 and 30120733).

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Tau (phospho S202) antibody [EPR2402] (ab108387) at 1/1000 dilution

Lane 1:

Human brain lysate at 15 µg

Lane 2:

Human brain lysate then the membrane treated with Alkaline Phosphatase for 1 hour at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 78 kDa

Observed band size: 32-72 kDa

false

• WB

Supplier Data

Western blot - Anti-Tau (phospho S202) antibody [EPR2402] (AB108387)

The molecular weight observed is consistent with what has been described in the literature (PMID : 28382304, 32692785 and 30120733).

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Tau (phospho S202) antibody [EPR2402] (ab108387) at 1/1000 dilution

Lane 1:

Rat hippocampus lysate at 15 µg

Lane 2:

Rat hippocampus lysate then the membrane treated with Alkaline Phosphatase for 1 hour at 15 µg

Lane 3:

Rat cerebral cortex lysate at 15 µg

Lane 4:

Rat cerebral cortex lysate then the membrane treated with Alkaline Phosphatase for 1 hour at 15 µg

Lane 5:

Mouse hippocampus lysate at 15 µg

Lane 6:

Mouse hippocampus lysate then the membrane treated with Alkaline Phosphatase for 1 hour at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 78 kDa

Observed band size: 32-72 kDa

false

• Dot

Supplier Data

Dot Blot - Anti-Tau (phospho S202) antibody [EPR2402] (AB108387)

Dot blot analysis using 1/1000 dilution ab108387 and Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary at 1/100000 dilution.

Blocking and diluting buffer : 5% NFDM/TBST

Lane 1 : Tau non-phospho peptide

Lane 2 : Tau S199 phospho peptide

Lane 3 : Tau S202 phospho peptide

Lane 4 : Tau S199+S202 phospho peptide

Exposure time : 3 minutes

• WB

CiteAb

Western blot - Anti-Tau (phospho S202) antibody [EPR2402] (AB108387)

Tau (phospho S202) western blot using anti-Tau (phospho S202) antibody [EPR2402] ab108387. Publication image and figure legend from Sun, W., Lee, S., et al., 2016, Sci Rep, PubMed 27708431.

ab108387 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab108387 please see the product overview.

MB reduced Tau phosphorylation by endogenous kinases in 293T cells.(a) Representative immunoblot of lysates from 293T cells transfected with 4R2N Tau. Transfected cells were treated with AC, MB and OS for 3 h at concentrations indicated in the panel. The levels of pTau S262, S396, S214, S202 and total Tau were reported with β-actin as the loading control. (b–e) Quantification of the ratio of pTau to total Tau presented as the percent of the level found in cells transfected with Tau but without drug treatment. Results are mean ± SD (n = 3). *0.01 < p < 0.05; **0.001 < p < 0.01 ***p < 0.001. Student's t test is conducted between corresponding values in AC and OS or MB and OS group.

false