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Rabbit Recombinant Monoclonal TAU phospho S202 antibody. Suitable for Dot, WB and reacts with Synthetic peptide, Mouse, Rat, Human samples. Cited in 16 publications.


Images

Western blot - Anti-Tau (phospho S202) antibody [EPR2402] (AB108387), expandable thumbnail
  • Western blot - Anti-Tau (phospho S202) antibody [EPR2402] (AB108387), expandable thumbnail
  • Dot Blot - Anti-Tau (phospho S202) antibody [EPR2402] (AB108387), expandable thumbnail
  • Western blot - Anti-Tau (phospho S202) antibody [EPR2402] (AB108387), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
DotICC/IFFlow CytWBIHC-P
Human
Expected
Not recommended
Not recommended
Tested
Not recommended
Mouse
Expected
Not recommended
Not recommended
Tested
Not recommended
Rat
Expected
Not recommended
Not recommended
Tested
Not recommended
Synthetic peptide
Tested
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Synthetic peptide
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Mouse, Rat, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Synthetic peptide
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/5000 - 1/10000
Notes

-

Species
Rat
Dilution info
1/5000 - 1/10000
Notes

-

Species
Human
Dilution info
1/5000 - 1/10000
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Synthetic peptide
Dilution info
-
Notes

-

Associated Products

Select an associated product type

7 products for Alternative Product

Target data

Function

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed:21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed:21985311, PubMed:32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

Additional Targets

MAPT phospho S202

Alternative names

MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

Recommended products

Rabbit Recombinant Monoclonal TAU phospho S202 antibody. Suitable for Dot, WB and reacts with Synthetic peptide, Mouse, Rat, Human samples. Cited in 16 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR2402
Purification technique
Affinity purification Protein A
Specificity
Stimulation may be required to allow detection of the phosphorylated protein. Please see images below for recommended treatment conditions and positive controls.

The specificity of this antibody refers to P10636-8.

Dissociation constant
4.13 x 10-12 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.

Biological function summary

Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.

Pathways

Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.

Associated diseases and disorders

Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-Tau (phospho S202) antibody [EPR2402] (ab108387), expandable thumbnail

    Western blot - Anti-Tau (phospho S202) antibody [EPR2402] (ab108387)

    The molecular weight observed is consistent with what has been described in the literature (PMID: 28382304, 32692785 and 30120733).

    Blocking and diluting buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-Tau (phospho S202) antibody [EPR2402] (ab108387) at 1/1000 dilution

    Lane 1: Rat hippocampus lysate at 15 µg

    Lane 2: Rat hippocampus lysate then the membrane treated with Alkaline Phosphatase for 1 hour at 15 µg

    Lane 3: Rat cerebral cortex lysate at 15 µg

    Lane 4: Rat cerebral cortex lysate then the membrane treated with Alkaline Phosphatase for 1 hour at 15 µg

    Lane 5: Mouse hippocampus lysate at 15 µg

    Lane 6: Mouse hippocampus lysate then the membrane treated with Alkaline Phosphatase for 1 hour at 15 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 78 kDa

    Observed band size: 32-72 kDa

  • Western blot - Anti-Tau (phospho S202) antibody [EPR2402] (ab108387), expandable thumbnail

    Western blot - Anti-Tau (phospho S202) antibody [EPR2402] (ab108387)

    The molecular weight observed is consistent with what has been described in the literature (PMID: 28382304, 32692785 and 30120733).

    Blocking and diluting buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-Tau (phospho S202) antibody [EPR2402] (ab108387) at 1/1000 dilution

    Lane 1: Human brain lysate at 15 µg

    Lane 2: Human brain lysate then the membrane treated with Alkaline Phosphatase for 1 hour at 15 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 78 kDa

    Observed band size: 32-72 kDa

  • Dot Blot - Anti-Tau (phospho S202) antibody [EPR2402] (ab108387), expandable thumbnail

    Dot Blot - Anti-Tau (phospho S202) antibody [EPR2402] (ab108387)

    Dot blot analysis using 1/1000 dilution ab108387 and Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary at 1/100000 dilution.

    Blocking and diluting buffer: 5% NFDM/TBST

    Lane 1: Tau non-phospho peptide

    Lane 2: Tau S199 phospho peptide

    Lane 3: Tau S202 phospho peptide

    Lane 4: Tau S199+S202 phospho peptide

    Exposure time: 3 minutes

  • Western blot - Anti-Tau (phospho S202) antibody [EPR2402] (ab108387), expandable thumbnail

    Image collected and cropped by CiteAb under a CC-BY license from the publication

    Western blot - Anti-Tau (phospho S202) antibody [EPR2402] (ab108387)

    Tau (phospho S202) western blot using anti-Tau (phospho S202) antibody [EPR2402] ab108387. Publication image and figure legend from Sun, W., Lee, S., et al., 2016, Sci Rep, PubMed 27708431.


    ab108387 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab108387 please see the product overview.

    MB reduced Tau phosphorylation by endogenous kinases in 293T cells.(a) Representative immunoblot of lysates from 293T cells transfected with 4R2N Tau. Transfected cells were treated with AC, MB and OS for 3 h at concentrations indicated in the panel. The levels of pTau S262, S396, S214, S202 and total Tau were reported with β-actin as the loading control. (b–e) Quantification of the ratio of pTau to total Tau presented as the percent of the level found in cells transfected with Tau but without drug treatment. Results are mean ± SD (n = 3). *0.01 < P < 0.05; **0.001 < P < 0.01 ***P < 0.001. Student’s t test is conducted between corresponding values in AC and OS or MB and OS group.

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