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AB196359

Anti-Tau (phospho S202) antibody [EPR2402] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal TAU phospho S202 antibody. Carrier free. Suitable for Dot, WB and reacts with Synthetic peptide, Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau, MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

3 Images
Western blot - Anti-Tau (phospho S202) antibody [EPR2402] - BSA and Azide free (AB196359)
  • WB

Supplier Data

Western blot - Anti-Tau (phospho S202) antibody [EPR2402] - BSA and Azide free (AB196359)

The molecular weight observed is consistent with what has been described in the literature (PMID : 28382304, 32692785 and 30120733).

Blocking and diluting buffer : 5% NFDM/TBST

This data was developed using the same antibody clone in a different buffer formulation containing Tris glycine, BSA, glycerol, and sodium azide (ab108387)

All lanes:

Western blot - Anti-Tau (phospho S202) antibody [EPR2402] (<a href='/en-us/products/primary-antibodies/tau-phospho-s202-antibody-epr2402-ab108387'>ab108387</a>) at 1/1000 dilution

Lane 1:

Human brain lysate at 15 µg

Lane 2:

Human brain lysate then the membrane treated with Alkaline Phosphatase for 1 hour at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 78 kDa

Observed band size: 32-72 kDa

false

Western blot - Anti-Tau (phospho S202) antibody [EPR2402] - BSA and Azide free (AB196359)
  • WB

Supplier Data

Western blot - Anti-Tau (phospho S202) antibody [EPR2402] - BSA and Azide free (AB196359)

The molecular weight observed is consistent with what has been described in the literature (PMID : 28382304, 32692785 and 30120733).

Blocking and diluting buffer : 5% NFDM/TBST

This data was developed using the same antibody clone in a different buffer formulation containing Tris glycine, BSA, glycerol, and sodium azide (ab108387)

All lanes:

Western blot - Anti-Tau (phospho S202) antibody [EPR2402] (<a href='/en-us/products/primary-antibodies/tau-phospho-s202-antibody-epr2402-ab108387'>ab108387</a>) at 1/1000 dilution

Lane 1:

Rat hippocampus lysate at 15 µg

Lane 2:

Rat hippocampus lysate then the membrane treated with Alkaline Phosphatase for 1 hour at 15 µg

Lane 3:

Rat cerebral cortex lysate at 15 µg

Lane 4:

Rat cerebral cortex lysate then the membrane treated with Alkaline Phosphatase for 1 hour at 15 µg

Lane 5:

Mouse hippocampus lysate at 15 µg

Lane 6:

Mouse hippocampus lysate then the membrane treated with Alkaline Phosphatase for 1 hour at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 78 kDa

Observed band size: 32-72 kDa

false

Dot Blot - Anti-Tau (phospho S202) antibody [EPR2402] - BSA and Azide free (AB196359)
  • Dot

Supplier Data

Dot Blot - Anti-Tau (phospho S202) antibody [EPR2402] - BSA and Azide free (AB196359)

Dot blot analysis using 1/1000 dilution ab108387 and Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary at 1/100000 dilution.

Blocking and diluting buffer : 5% NFDM/TBST

Lane 1 : Tau non-phospho peptide

Lane 2 : Tau S199 phospho peptide

Lane 3 : Tau S202 phospho peptide

Lane 4 : Tau S199+S202 phospho peptide

Exposure time : 3 minutes

This data was developed using the same antibody clone in a different buffer formulation containing Tris glycine, BSA, glycerol, and sodium azide (ab108387).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2402

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

Dot, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

<p><em>Stimulation may be required to allow detection of the phosphorylated protein. Please see images below for recommended treatment conditions and positive controls.</em></p><p>The specificity of this antibody refers to P10636-8&#46;</p>

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Reactivity data

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Product details

ab196359 is the carrier-free version of ab108387.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.
Biological function summary

Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.

Pathways

Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.

Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed : 21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed : 21985311, PubMed : 32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
See full target information MAPT phospho S202

Additional targets

MAPT phospho S202 + T205
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Bio-protocol 15:e5424 PubMed40873476

2025

Assessing Human Treg Suppression at Single-Cell Resolution Using Mass Cytometry.

Applications

Unspecified application

Species

Unspecified reactive species

Jonas Nørskov Søndergaard,Janyerkye Tulyeu,David Priest,Shimon Sakaguchi,James B Wing
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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