Rabbit Recombinant Monoclonal TAU phospho S202 + T205 antibody. Suitable for IP, Dot, WB and reacts with Human, Synthetic peptide samples. Cited in 5 publications.
Images
![Immunoprecipitation - Anti-Tau (phospho S202 + T205) antibody [EPR20390] (AB210703), expandable thumbnail](https://content.abcam.com/products/images/tau-phospho-s202-t205-antibody-epr20390-ab210703--immunoprecipitation-img11050.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Tau (phospho S202 + T205) antibody [EPR20390] (AB210703)")
![Western blot - Anti-Tau (phospho S202 + T205) antibody [EPR20390] (AB210703), expandable thumbnail](https://content.abcam.com/products/images/tau-phospho-s202-t205-antibody-epr20390-ab210703--western-blot-img40227.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Tau (phospho S202 + T205) antibody [EPR20390] (AB210703)")
![Dot Blot - Anti-Tau (phospho S202 + T205) antibody [EPR20390] (AB210703), expandable thumbnail](https://content.abcam.com/products/images/tau-phospho-s202-t205-antibody-epr20390-ab210703--dot-blot-img2411.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-Tau (phospho S202 + T205) antibody [EPR20390] (AB210703)")
![Western blot - Anti-Tau (phospho S202 + T205) antibody [EPR20390] (AB210703), expandable thumbnail](https://content.abcam.com/products/images/tau-phospho-s202-t205-antibody-epr20390-ab210703--western-blot-img424992.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Tau (phospho S202 + T205) antibody [EPR20390] (AB210703)")
Publications
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- Nature neuroscience 26:1365-13782023Heart failure-induced cognitive dysfunction is mediated by intracellular Ca leak through ryanodine receptor type 2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaikel Dridi,Yang Liu,Steven Reiken,Xiaoping Liu,Elentina K Argyrousi,Qi Yuan,Marco C Miotto,Leah Sittenfeld,Andrei Meddar,Rajesh Kumar Soni,Ottavio Arancio,Alain Lacampagne,Andrew R MarksPubMed 37429912
- iScience 26:1063752023INPP5D modulates TREM2 loss-of-function phenotypes in a β-amyloidosis mouse model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAkihiro Iguchi,Sho Takatori,Shingo Kimura,Hiroki Muneto,Kai Wang,Hayato Etani,Genta Ito,Haruaki Sato,Yukiko Hori,Junko Sasaki,Takashi Saito,Takaomi C Saido,Tsuneya Ikezu,Toshiyuki Takai,Takehiko Sasaki,Taisuke TomitaPubMed 37035000
- Bio-protocol 13:e45942023Methods to detect AUTOphagy-Targeting Chimera (AUTOTAC)-mediated Targeted Protein Degradation in Tauopathies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMin Ju Lee,Su Bin Kim,Hee Yeon Kim,Su Jin Lee,Ji Su Lee,Yong Tae Kwon,Chang Hoon JiPubMed 36789169
- Journal of healthcare engineering 2022:46024282022Neuroprotective Effects of Probiotic-Supplemented Diet on Cognitive Behavior of 3xTg-AD Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChenxi Tan,Yang Liu,Huiyi Zhang,Cihan Di,Dechao Xu,Chunming Liang,Ningning Zhang,Bing Han,Weiya LangPubMed 35035837
- Journal of Alzheimer's disease reports 5:283-2942021Aluminum and Tau in Neurofibrillary Tangles in Familial Alzheimer's Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMatthew John Mold,Adam O'Farrell,Benjamin Morris,Christopher ExleyPubMed 34113785
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Dot | WB | |
|---|---|---|---|
| Human | Tested | Expected | Tested |
| Synthetic peptide | Not recommended | Tested | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/30 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
The protein expressed by the MAPT gene promotes microtubule assembly and stability and might be involved in establishing and maintaining neuronal polarity. Its C-terminus binds axonal microtubules, and the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between the two. Axonal polarity is predetermined by MAPT localization within the neuronal cell's domain defined by the centrosome. The short isoforms allow cytoskeleton plasticity, whereas the longer isoforms may preferentially play a role in its stabilization. This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau
Recommended products
Rabbit Recombinant Monoclonal TAU phospho S202 + T205 antibody. Suitable for IP, Dot, WB and reacts with Human, Synthetic peptide samples. Cited in 5 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR20390
- Purification technique
- Affinity purification Protein A
- Specificity
The specificity of this antibody refers to P10636-8.
- Dissociation constant
- 1.65 x 10-11 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.
- Biological function summary
Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.
- Pathways
Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.
- Associated diseases and disorders
Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunoprecipitation - Anti-Tau (phospho S202 + T205) antibody [EPR20390] (ab210703), expandable thumbnail](https://content.abcam.com/products/images/tau-phospho-s202-t205-antibody-epr20390-ab210703--immunoprecipitation-img11050.jpg "Immunoprecipitation - Anti-Tau (phospho S202 + T205) antibody [EPR20390] (ab210703)")
Immunoprecipitation - Anti-Tau (phospho S202 + T205) antibody [EPR20390] (ab210703)
Tau (phospho S202 + T205) was immunoprecipitated from 0.35 mg of human brain lysate with ab210703 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab210703 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.
Lane 1: Human brain lysate 10 μg (Input).
Lane 2: ab210703 IP in human brain lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab210703 in human brain lysate.Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 8 seconds.
All lanes: Immunoprecipitation - Anti-Tau (phospho S202 + T205) antibody [EPR20390] (ab210703)
Predicted band size: 78 kDa
![Western blot - Anti-Tau (phospho S202 + T205) antibody [EPR20390] (ab210703), expandable thumbnail](https://content.abcam.com/products/images/tau-phospho-s202-t205-antibody-epr20390-ab210703--western-blot-img40227.jpg "Western blot - Anti-Tau (phospho S202 + T205) antibody [EPR20390] (ab210703)")
Western blot - Anti-Tau (phospho S202 + T205) antibody [EPR20390] (ab210703)
Blocking and dilution buffer: 5% NFDM/TBST.The molecular weight observed is consistent with what has been described in the literature (PMID: 21932121, PMID: 20660113).
All lanes: Western blot - Anti-Tau (phospho S202 + T205) antibody [EPR20390] (ab210703) at 1/1000 dilution
Lane 1: Human brain tissue lysate at 10 µg
Lane 2: Alkaline phosphatase treated human brain tissue lysate (1 hour) at 10 µg
SecondaryAll lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 78 kDa
Observed band size: 50-80 kDa
Exposure time: 3min
![Dot Blot - Anti-Tau (phospho S202 + T205) antibody [EPR20390] (ab210703), expandable thumbnail](https://content.abcam.com/products/images/tau-phospho-s202-t205-antibody-epr20390-ab210703--dot-blot-img2411.jpg "Dot Blot - Anti-Tau (phospho S202 + T205) antibody [EPR20390] (ab210703)")
Dot Blot - Anti-Tau (phospho S202 + T205) antibody [EPR20390] (ab210703)
Dot blot analysis of Tau (phospho S202+ T205) labeled with ab210703 at 1/1000 dilution.
Lane 1: Tau (phospho S202 + T205) peptide.
Lane 2: Tau (phospho S202) peptide.
Lane 3: Tau (phospho T205) peptide.
Lane 4: Tau non-phospho peptide.Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution was used as secondary antibody.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
![Western blot - Anti-Tau (phospho S202 + T205) antibody [EPR20390] (ab210703), expandable thumbnail](https://content.abcam.com/products/images/tau-phospho-s202-t205-antibody-epr20390-ab210703--western-blot-img424992.jpg "Western blot - Anti-Tau (phospho S202 + T205) antibody [EPR20390] (ab210703)")
Western blot - Anti-Tau (phospho S202 + T205) antibody [EPR20390] (ab210703)
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Tau (phospho S202 + T205) antibody [EPR20390] (ab210703) at 1/1000 dilution
Lane 1: 293T cells transfected with an empty vector containing a flag tag whole cell lysate, at 20 µg
Lane 2: 293T cells transfected with a human Tau expression vector containing a flag whole cell lysate at 20 µg
Lane 3: 293T cells transfected with a human MAP2 expression vector containing a flag whole cell lysate at 20 µg
Lane 4: 293T cells transfected with a human MAP4 expression vector containing a flag whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/200000 dilution
Predicted band size: 78 kDa
Observed band size: 55-100 kDa
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