Anti-Tau (phospho S202 + T205) antibody [EPR20390] - BSA and Azide free

• IP

Lab

Immunoprecipitation - Anti-Tau (phospho S202 + T205) antibody [EPR20390] - BSA and Azide free (AB237594)

Tau (phospho S202 + T205) was immunoprecipitated from 0.35 mg of human brain lysate with ab210703 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab210703 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : Human brain lysate 10 μg (Input).
Lane 2 : ab210703 IP in human brain lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab210703 in human brain lysate.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 8 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210703).

All lanes:

Immunoprecipitation - Anti-Tau (phospho S202 + T205) antibody [EPR20390] (<a href='/en-us/products/primary-antibodies/tau-phospho-s202-t205-antibody-epr20390-ab210703'>ab210703</a>)

Predicted band size: 78 kDa

false

• WB

Lab

Western blot - Anti-Tau (phospho S202 + T205) antibody [EPR20390] - BSA and Azide free (AB237594)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Tau (phospho S202 + T205) antibody [EPR20390] (<a href='/en-us/products/primary-antibodies/tau-phospho-s202-t205-antibody-epr20390-ab210703'>ab210703</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a flag tag whole cell lysate, at 20 µg

Lane 2:

293T cells transfected with a human Tau expression vector containing a flag whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a human MAP2 expression vector containing a flag whole cell lysate at 20 µg

Lane 4:

293T cells transfected with a human MAP4 expression vector containing a flag whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/200000 dilution

Predicted band size: 78 kDa

Observed band size: 55-100 kDa

false

• WB

Lab

Western blot - Anti-Tau (phospho S202 + T205) antibody [EPR20390] - BSA and Azide free (AB237594)

Blocking and dilution buffer : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID : 21932121, PMID : 20660113).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210703).

All lanes:

Western blot - Anti-Tau (phospho S202 + T205) antibody [EPR20390] (<a href='/en-us/products/primary-antibodies/tau-phospho-s202-t205-antibody-epr20390-ab210703'>ab210703</a>) at 1/1000 dilution

Lane 1:

Human brain tissue lysate at 10 µg

Lane 2:

Alkaline phosphatase treated human brain tissue lysate (1 hour) at 10 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/100000 dilution

Predicted band size: 78 kDa

Observed band size: 50-80 kDa

true

Exposure time: 3min

• Dot

Supplier Data

Dot Blot - Anti-Tau (phospho S202 + T205) antibody [EPR20390] - BSA and Azide free (AB237594)

Dot blot analysis of Tau (phospho S202+ T205) labeled with ab210703 at 1/1000 dilution.

Lane 1 : Tau (phospho S202 + T205) peptide.
Lane 2 : Tau (phospho S202) peptide.
Lane 3 : Tau (phospho T205) peptide.
Lane 4 : Tau non-phospho peptide.

Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210703).