Skip to main content

Rabbit Recombinant Monoclonal TAU phospho S214 antibody. Suitable for IHC-P, Dot, WB and reacts with Mouse, Human, Synthetic peptide samples. Cited in 12 publications.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] (AB170892), expandable thumbnail
  • Western blot - Anti-Tau (phospho S214) antibody [EPR1884(2)] (AB170892), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] (AB170892), expandable thumbnail
  • Western blot - Anti-Tau (phospho S214) antibody [EPR1884(2)] (AB170892), expandable thumbnail
  • Dot Blot - Anti-Tau (phospho S214) antibody [EPR1884(2)] (AB170892), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFIPFlow CytDotWB
Human
Tested
Not recommended
Not recommended
Not recommended
Expected
Tested
Mouse
Tested
Not recommended
Not recommended
Not recommended
Expected
Tested
Rat
Predicted
Not recommended
Not recommended
Not recommended
Predicted
Predicted
Synthetic peptide
Not recommended
Not recommended
Not recommended
Not recommended
Tested
Not recommended

Tested
Tested

Species
Mouse
Dilution info
1/100 - 1/250
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species
Human
Dilution info
1/100 - 1/250
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Synthetic peptide
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat, Synthetic peptide
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat, Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Synthetic peptide
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Mouse, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000 - 1/10000
Notes

-

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

Target data

Function

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed:21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed:21985311, PubMed:32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

Additional Targets

MAPT phospho S214

Alternative names

Recommended products

Rabbit Recombinant Monoclonal TAU phospho S214 antibody. Suitable for IHC-P, Dot, WB and reacts with Mouse, Human, Synthetic peptide samples. Cited in 12 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR1884(2)
Purification technique
Affinity purification Protein A
Specificity

The specificity of this antibody refers to P10636-8.

Dissociation constant
6.47 x 10-11 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.

Biological function summary

Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.

Pathways

Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.

Associated diseases and disorders

Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] (ab170892), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] (ab170892)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human AD cerebral cortex tissue labelling Tau with ab170892 at 1/100 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody. Counterstained with hematoxylin.

    Positive staining on human AD cerebral cortex without alkaline phosphatase treatment (image A). No signal was detected when tissues were treated with alkaline phosphatase (image B).

    The section was incubated with ab170892 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

  • Western blot - Anti-Tau (phospho S214) antibody [EPR1884(2)] (ab170892), expandable thumbnail

    Western blot - Anti-Tau (phospho S214) antibody [EPR1884(2)] (ab170892)

    Blocking/Diluting buffer and concentration: 5% NFDM/TBST

    All lanes: Western blot - Anti-Tau (phospho S214) antibody [EPR1884(2)] (ab170892) at 1/1000 dilution

    Lane 1: C57 mouse cerebral cortex whole cell lysates. at 15 µg

    Lane 2: C57 mouse cerebral cortex whole cell lysates. The membrane was incubated with phosphatase. at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 78 kDa

    Observed band size: 50-70 kDa

    Exposure time: 30s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] (ab170892), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] (ab170892)

    ab170892 showing +ve staining in Mouse brain tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-Tau (phospho S214) antibody [EPR1884(2)] (ab170892), expandable thumbnail

    Western blot - Anti-Tau (phospho S214) antibody [EPR1884(2)] (ab170892)

    All lanes: Western blot - Anti-Tau (phospho S214) antibody [EPR1884(2)] (ab170892) at 1/1000 dilution

    Lane 1: SH-SY5Y cell lysates untreated at 10 µg

    Lane 2: SH-SY5Y cell lysates treated with Okadic acid + Calyculin A. at 10 µg

    Secondary

    All lanes: Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 78 kDa

  • Dot Blot - Anti-Tau (phospho S214) antibody [EPR1884(2)] (ab170892), expandable thumbnail

    Dot Blot - Anti-Tau (phospho S214) antibody [EPR1884(2)] (ab170892)

    Dot blot analysis of Tau (phospho S214) phospho peptide (Lane 1) and Tau non-phospho peptide (Lane 2) labeling Tau (phospho S214) with ab170892 at a dilution of 1/1000. Goat Anti-Rabbit IgG H&L (HRP) ab97051 (Peroxidase conjugated goat anti-rabbit IgG) (H+L) at 1/100 000 was used as the secondary antibody.

    Blocking and diluting buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] (ab170892), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] (ab170892)

    Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling Tau (phospho S214) with ab170892 at 1/100 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] (ab170892), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] (ab170892)

    ab170892 showing -ve staining in Human normal spleen tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] (ab170892), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] (ab170892)

    ab170892 showing -ve staining in Human normal kidney tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] (ab170892), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] (ab170892)

    ab170892 showing -ve staining in Human cervical carcinoma tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com