Anti-Tau (phospho S214) antibody [EPR1884(2)] - BSA and Azide free (ab196358) is a rabbit recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation detecting Tau in Western Blot, IHC-P, Dot Blot. Suitable for Human, Mouse.
- BSA, sodium azide, and glycerol-free for easy conjugation
- Biophysical QC for unrivalled batch-batch consistency
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] - BSA and Azide free (AB196358), expandable thumbnail](https://content.abcam.com/products/images/tau-phospho-s214-antibody-epr18842-bsa-and-azide-free-ab196358--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img104059.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] - BSA and Azide free (AB196358)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] - BSA and Azide free (AB196358), expandable thumbnail](https://content.abcam.com/products/images/tau-phospho-s214-antibody-epr18842-bsa-and-azide-free-ab196358--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130888.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] - BSA and Azide free (AB196358)")
![Dot Blot - Anti-Tau (phospho S214) antibody [EPR1884(2)] - BSA and Azide free (AB196358), expandable thumbnail](https://content.abcam.com/products/images/tau-phospho-s214-antibody-epr18842-bsa-and-azide-free-ab196358--dot-blot-img807.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-Tau (phospho S214) antibody [EPR1884(2)] - BSA and Azide free (AB196358)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] - BSA and Azide free (AB196358), expandable thumbnail](https://content.abcam.com/products/images/tau-phospho-s214-antibody-epr18842-bsa-and-azide-free-ab196358--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img92260.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] - BSA and Azide free (AB196358)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] - BSA and Azide free (AB196358), expandable thumbnail](https://content.abcam.com/products/images/tau-phospho-s214-antibody-epr18842-bsa-and-azide-free-ab196358--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img64977.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] - BSA and Azide free (AB196358)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | Dot | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Expected | Expected | Tested | Not recommended |
| Mouse | Not recommended | Not recommended | Predicted | Expected | Predicted | Not recommended |
| Rat | Not recommended | Not recommended | Predicted | Predicted | Predicted | Not recommended |
| Synthetic peptide | Not recommended | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat, Synthetic peptide | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat, Synthetic peptide | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat, Synthetic peptide | Dilution info - | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
Target data
Function
The protein expressed by the MAPT gene promotes microtubule assembly and stability and might be involved in establishing and maintaining neuronal polarity. Its C-terminus binds axonal microtubules, and the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between the two. Axonal polarity is predetermined by MAPT localization within the neuronal cell's domain defined by the centrosome. The short isoforms allow cytoskeleton plasticity, whereas the longer isoforms may preferentially play a role in its stabilization. This supplementary information is collated from multiple sources and compiled automatically.
Additional Targets
MAPT phospho S214
Alternative names
MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau
Recommended products
Anti-Tau (phospho S214) antibody [EPR1884(2)] - BSA and Azide free (ab196358) is a rabbit recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation detecting Tau in Western Blot, IHC-P, Dot Blot. Suitable for Human, Mouse.
- BSA, sodium azide, and glycerol-free for easy conjugation
- Biophysical QC for unrivalled batch-batch consistency
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR1884(2)
- Purification technique
- Affinity purification Protein A
- Specificity
The specificity of this antibody refers to P10636-8.
- Dissociation constant
- 6.47 x 10-11 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
Anti-Tau (phospho S214) antibody [EPR1884(2)] - BSA and Azide free (ab196358) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Dot Blot in Human, Mouse samples.
What is the molecular weight of Tau?
Anti-Tau (phospho S214) [EPR1884(2)] - BSA and Azide free (ab196358) specifically detects a band for Tau (UniProt: P10636) at a molecular weight of 78kDa.
Other related products
We have a range of other formats of antibody clone [EPR1884(2)] also available for your convenience:
Anti-Tau (phospho S214) antibody [EPR1884(2)] ab170892, Carrier free - ab196358
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.
- Biological function summary
Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.
- Pathways
Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3Β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.
- Associated diseases and disorders
Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] - BSA and Azide free (ab196358), expandable thumbnail](https://content.abcam.com/products/images/tau-phospho-s214-antibody-epr18842-bsa-and-azide-free-ab196358--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img104059.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] - BSA and Azide free (ab196358)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] - BSA and Azide free (ab196358)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human AD cerebral cortex tissue labelling Tau with Anti-Tau (phospho S214) antibody [EPR1884(2)] ab170892 at 1/100 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody. Counterstained with hematoxylin.
Positive staining on human AD cerebral cortex without alkaline phosphatase treatment (image A). No signal was detected when tissues were treated with alkaline phosphatase (image B).
The section was incubated with Anti-Tau (phospho S214) antibody [EPR1884(2)] ab170892 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] - BSA and Azide free (ab196358), expandable thumbnail](https://content.abcam.com/products/images/tau-phospho-s214-antibody-epr18842-bsa-and-azide-free-ab196358--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130888.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] - BSA and Azide free (ab196358)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] - BSA and Azide free (ab196358)
Anti-Tau (phospho S214) antibody [EPR1884(2)] ab170892 showing +ve staining in Mouse brain tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Tau (phospho S214) antibody [EPR1884(2)] ab170892).
![Dot Blot - Anti-Tau (phospho S214) antibody [EPR1884(2)] - BSA and Azide free (ab196358), expandable thumbnail](https://content.abcam.com/products/images/tau-phospho-s214-antibody-epr18842-bsa-and-azide-free-ab196358--dot-blot-img807.jpg "Dot Blot - Anti-Tau (phospho S214) antibody [EPR1884(2)] - BSA and Azide free (ab196358)")
Dot Blot - Anti-Tau (phospho S214) antibody [EPR1884(2)] - BSA and Azide free (ab196358)
Dot blot analysis of Tau (phospho S214) phospho peptide (Lane 1) and Tau non-phospho peptide (Lane 2) labeling Tau (phospho S214) with Anti-Tau (phospho S214) antibody [EPR1884(2)] ab170892 at a dilution of 1/1000. Goat Anti-Rabbit IgG H&L (HRP) ab97051 (Peroxidase conjugated goat anti-rabbit IgG) (H+L) at 1/100 000 was used as the secondary antibody.
Blocking and diluting buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Tau (phospho S214) antibody [EPR1884(2)] ab170892).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] - BSA and Azide free (ab196358), expandable thumbnail](https://content.abcam.com/products/images/tau-phospho-s214-antibody-epr18842-bsa-and-azide-free-ab196358--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img92260.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] - BSA and Azide free (ab196358)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] - BSA and Azide free (ab196358)
Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling Tau (phospho S214) with Anti-Tau (phospho S214) antibody [EPR1884(2)] ab170892 at 1/100 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Tau (phospho S214) antibody [EPR1884(2)] ab170892).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] - BSA and Azide free (ab196358), expandable thumbnail](https://content.abcam.com/products/images/tau-phospho-s214-antibody-epr18842-bsa-and-azide-free-ab196358--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img64977.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] - BSA and Azide free (ab196358)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] - BSA and Azide free (ab196358)
Anti-Tau (phospho S214) antibody [EPR1884(2)] ab170892 showing -ve staining in Human normal spleen tissue.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Tau (phospho S214) antibody [EPR1884(2)] ab170892).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] - BSA and Azide free (ab196358), expandable thumbnail](https://content.abcam.com/products/images/tau-phospho-s214-antibody-epr18842-bsa-and-azide-free-ab196358--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img93726.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] - BSA and Azide free (ab196358)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] - BSA and Azide free (ab196358)
Anti-Tau (phospho S214) antibody [EPR1884(2)] ab170892 showing -ve staining in Human normal kidney tissue.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Tau (phospho S214) antibody [EPR1884(2)] ab170892).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] - BSA and Azide free (ab196358), expandable thumbnail](https://content.abcam.com/products/images/tau-phospho-s214-antibody-epr18842-bsa-and-azide-free-ab196358--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img125682.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] - BSA and Azide free (ab196358)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] - BSA and Azide free (ab196358)
Anti-Tau (phospho S214) antibody [EPR1884(2)] ab170892 showing -ve staining in Human cervical carcinoma tissue.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Tau (phospho S214) antibody [EPR1884(2)] ab170892).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] - BSA and Azide free (ab196358), expandable thumbnail](https://content.abcam.com/products/images/tau-phospho-s214-antibody-epr18842-bsa-and-azide-free-ab196358--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img93726.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] - BSA and Azide free (ab196358)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] - BSA and Azide free (ab196358), expandable thumbnail](https://content.abcam.com/products/images/tau-phospho-s214-antibody-epr18842-bsa-and-azide-free-ab196358--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img125682.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] - BSA and Azide free (ab196358)")
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