AB244235

Anti-Tau (phospho S235) antibody [EPR2452] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal TAU phospho S235 antibody. Carrier free. Suitable for I-ELISA, WB, Dot and reacts with Human, Mouse, Rat, Synthetic peptide samples. Cited in 1 publication.

View Alternative Names

MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau, MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

5 Images

Indirect ELISA - Anti-Tau (phospho S235) antibody [EPR2452] - BSA and Azide free (AB244235)

I-ELISA

Lab

Indirect ELISA - Anti-Tau (phospho S235) antibody [EPR2452] - BSA and Azide free (AB244235)

This data was developed using ab133253, the same antibody clone in a different buffer formulation.

Indirect ELISA analysis of ab133253 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1 : 2500 dilution.

Antigen : Human Tau (phospho S235) peptide,Human Tau non-phospho peptide

Antigen concentration : 1000 ng/ml.

Unknown

Western blot - Anti-Tau (phospho S235) antibody [EPR2452] - BSA and Azide free (AB244235)

This data was developed using ab133253, the same antibody clone in a different buffer formulation.

Beta tubulin is shown as loading control in the bottom panel.

All lanes:

Western blot - Anti-Tau (phospho S235) antibody [EPR2452] (<a href='/en-us/products/primary-antibodies/tau-phospho-s235-antibody-epr2452-ab133253'>ab133253</a>) at 1/1000 dilution

Lane 1:

SH SY5Y cell lysates at 10 µg

Lane 2:

SH SY5Y cell lysates treated with Okadaic acid and Calyculin A. at 10 µg

Predicted band size: 78 kDa

false

Lab

Western blot - Anti-Tau (phospho S235) antibody [EPR2452] - BSA and Azide free (AB244235)

This data was developed using ab133253, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Tau (phospho S235) antibody [EPR2452] (<a href='/en-us/products/primary-antibodies/tau-phospho-s235-antibody-epr2452-ab133253'>ab133253</a>) at 1/1000 dilution

Lane 1:

Human hippocampus lysate at 10 µg

Lane 2:

Human hippocampus lysate - membrane incubated with phosphatase at 10 µg

Secondary

All lanes:

VeriBlot for IP secondary antibody (HRP) at 1/2000 dilution

Predicted band size: 78 kDa

Observed band size: 55 kDa

false

Exposure time: 3min

Lab

Western blot - Anti-Tau (phospho S235) antibody [EPR2452] - BSA and Azide free (AB244235)

This data was developed using ab133253, the same antibody clone in a different buffer formulation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Anti-Tau antibody [EP2456Y] (ab76128) - Total Protein Control (ab76128) at 1/1000 dilution.

All lanes:

Western blot - Anti-Tau (phospho S235) antibody [EPR2452] (<a href='/en-us/products/primary-antibodies/tau-phospho-s235-antibody-epr2452-ab133253'>ab133253</a>) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate (untreated membrane) at 20 µg

Lane 2:

Rat brain tissue lysate (untreated membrane) at 20 µg

Lane 3:

Mouse brain tissue lysate (alkaline phosphatase treated membrane) at 20 µg

Lane 4:

Rat brain tissue lysate (alkaline phosphatase treated membrane) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 35-80 kDa

false

Exposure time: 180s

Lab

Dot Blot - Anti-Tau (phospho S235) antibody [EPR2452] - BSA and Azide free (AB244235)

This data was developed using ab133253, the same antibody clone in a different buffer formulation.

Dot blot analysis of Tau (pS235) peptide (Lane 1) and Tau non-phospho peptide (Lane 2) labelling Tau (pS235) with ab133253 at a dilution of 1/1000. ab97051 (Peroxidase-conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/100000.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

Unconjugated

Anti-Tau (phospho S235) antibody [EPR2452]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2452

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

I-ELISA, Dot, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The specificity of this antibody refers to P10636-8.

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Reactivity data

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Product details

ab244235 is the carrier-free version of ab133253.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.

Biological function summary

Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.

Pathways

Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.

Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed : 21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed : 21985311, PubMed : 32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

See full target information MAPT phospho S235

Additional targets

MAPT phospho S235

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:112992 PubMed40687785

2025

Differential substrate degradation by super-degron: EGFP in wild-type mouse cells, PD-1 requires CRBN humanization.

Applications

Unspecified application

Species

Unspecified reactive species

Chie Naruse,Ojiro Ishibashi,Masatoshi Ohgushi,Hirohiko Imai,Tomoko Matsuzaki,Xuchi Pan,Tatsuhiko Miyazaki,Yuka Shidahara,Yu Shirakawa,Fumihiro Sugiyama,Masahide Asano

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Tau (phospho S235) antibody [EPR2452] - BSA and Azide free

Indirect ELISA - Anti-Tau (phospho S235) antibody [EPR2452] - BSA and Azide free (AB244235)

Western blot - Anti-Tau (phospho S235) antibody [EPR2452] - BSA and Azide free (AB244235)

Western blot - Anti-Tau (phospho S235) antibody [EPR2452] - BSA and Azide free (AB244235)

Western blot - Anti-Tau (phospho S235) antibody [EPR2452] - BSA and Azide free (AB244235)

Dot Blot - Anti-Tau (phospho S235) antibody [EPR2452] - BSA and Azide free (AB244235)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (1)

Product promise