Rabbit Recombinant Monoclonal TAU phospho S235 antibody. Carrier free. Suitable for I-ELISA, WB, Dot and reacts with Human, Mouse, Rat, Synthetic peptide samples.
Images
![Western blot - Anti-Tau (phospho S235) antibody [EPR2452] - BSA and Azide free (AB244235), expandable thumbnail](https://content.abcam.com/products/images/tau-phospho-s235-antibody-epr2452-bsa-and-azide-free-ab244235--western-blot-img19289.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Tau (phospho S235) antibody [EPR2452] - BSA and Azide free (AB244235)")
![Dot Blot - Anti-Tau (phospho S235) antibody [EPR2452] - BSA and Azide free (AB244235), expandable thumbnail](https://content.abcam.com/products/images/tau-phospho-s235-antibody-epr2452-bsa-and-azide-free-ab244235--dot-blot-img52253.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-Tau (phospho S235) antibody [EPR2452] - BSA and Azide free (AB244235)")
![Western blot - Anti-Tau (phospho S235) antibody [EPR2452] - BSA and Azide free (AB244235), expandable thumbnail](https://content.abcam.com/products/images/tau-phospho-s235-antibody-epr2452-bsa-and-azide-free-ab244235--western-blot-img9399.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Tau (phospho S235) antibody [EPR2452] - BSA and Azide free (AB244235)")
![Western blot - Anti-Tau (phospho S235) antibody [EPR2452] - BSA and Azide free (AB244235), expandable thumbnail](https://content.abcam.com/products/images/tau-phospho-s235-antibody-epr2452-bsa-and-azide-free-ab244235--western-blot-img445969.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Tau (phospho S235) antibody [EPR2452] - BSA and Azide free (AB244235)")
![Indirect ELISA - Anti-Tau (phospho S235) antibody [EPR2452] - BSA and Azide free (AB244235), expandable thumbnail](https://content.abcam.com/products/images/tau-phospho-s235-antibody-epr2452-bsa-and-azide-free-ab244235--indirect-elisa-img445970.png/_jcr_content/renditions/webp-475.webp "Indirect ELISA - Anti-Tau (phospho S235) antibody [EPR2452] - BSA and Azide free (AB244235)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| I-ELISA | WB | IP | Flow Cyt | Dot | IHC-P | ICC/IF | |
|---|---|---|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Not recommended | Expected | Not recommended | Not recommended |
| Mouse | Expected | Tested | Not recommended | Not recommended | Expected | Not recommended | Not recommended |
| Rat | Expected | Tested | Not recommended | Not recommended | Expected | Not recommended | Not recommended |
| Synthetic peptide | Not recommended | Not recommended | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
I-ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Synthetic peptide | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Synthetic peptide | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Synthetic peptide | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Synthetic peptide | Dilution info - | Notes - |
Target data
Function
The protein expressed by the MAPT gene promotes microtubule assembly and stability and might be involved in establishing and maintaining neuronal polarity. Its C-terminus binds axonal microtubules, and the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between the two. Axonal polarity is predetermined by MAPT localization within the neuronal cell's domain defined by the centrosome. The short isoforms allow cytoskeleton plasticity, whereas the longer isoforms may preferentially play a role in its stabilization. This supplementary information is collated from multiple sources and compiled automatically.
Additional Targets
MAPT phospho S235
Alternative names
MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau
Recommended products
Rabbit Recombinant Monoclonal TAU phospho S235 antibody. Carrier free. Suitable for I-ELISA, WB, Dot and reacts with Human, Mouse, Rat, Synthetic peptide samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR2452
- Purification technique
- Affinity purification Protein A
- Specificity
The specificity of this antibody refers to P10636-8.
- Dissociation constant
- 1.78 x 10-11 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab244235 is the carrier-free version of Anti-Tau (phospho S235) antibody [EPR2452] ab133253.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.
- Biological function summary
Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.
- Pathways
Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.
- Associated diseases and disorders
Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-Tau (phospho S235) antibody [EPR2452] - BSA and Azide free (ab244235), expandable thumbnail](https://content.abcam.com/products/images/tau-phospho-s235-antibody-epr2452-bsa-and-azide-free-ab244235--western-blot-img19289.jpg "Western blot - Anti-Tau (phospho S235) antibody [EPR2452] - BSA and Azide free (ab244235)")
Western blot - Anti-Tau (phospho S235) antibody [EPR2452] - BSA and Azide free (ab244235)
This data was developed using Anti-Tau (phospho S235) antibody [EPR2452] ab133253, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Tau (phospho S235) antibody [EPR2452] (Anti-Tau (phospho S235) antibody [EPR2452] ab133253) at 1/1000 dilution
Lane 1: Human hippocampus lysate at 10 µg
Lane 2: Human hippocampus lysate - membrane incubated with phosphatase at 10 µg
SecondaryAll lanes: VeriBlot for IP secondary antibody (HRP) at 1/2000 dilution
Predicted band size: 78 kDa
Observed band size: 55 kDa
Exposure time: 3min
![Dot Blot - Anti-Tau (phospho S235) antibody [EPR2452] - BSA and Azide free (ab244235), expandable thumbnail](https://content.abcam.com/products/images/tau-phospho-s235-antibody-epr2452-bsa-and-azide-free-ab244235--dot-blot-img52253.jpg "Dot Blot - Anti-Tau (phospho S235) antibody [EPR2452] - BSA and Azide free (ab244235)")
Dot Blot - Anti-Tau (phospho S235) antibody [EPR2452] - BSA and Azide free (ab244235)
This data was developed using Anti-Tau (phospho S235) antibody [EPR2452] ab133253, the same antibody clone in a different buffer formulation.Dot blot analysis of Tau (pS235) peptide (Lane 1) and Tau non-phospho peptide (Lane 2) labelling Tau (pS235) with Anti-Tau (phospho S235) antibody [EPR2452] ab133253 at a dilution of 1/1000. Goat Anti-Rabbit IgG H&L (HRP) ab97051 (Peroxidase-conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/100000. Blocking and dilution buffer: 5% NFDM/TBST. Exposure time: 3 minutes.
![Western blot - Anti-Tau (phospho S235) antibody [EPR2452] - BSA and Azide free (ab244235), expandable thumbnail](https://content.abcam.com/products/images/tau-phospho-s235-antibody-epr2452-bsa-and-azide-free-ab244235--western-blot-img9399.jpg "Western blot - Anti-Tau (phospho S235) antibody [EPR2452] - BSA and Azide free (ab244235)")
Western blot - Anti-Tau (phospho S235) antibody [EPR2452] - BSA and Azide free (ab244235)
This data was developed using Anti-Tau (phospho S235) antibody [EPR2452] ab133253, the same antibody clone in a different buffer formulation.
Beta tubulin is shown as loading control in the bottom panel.
All lanes: Western blot - Anti-Tau (phospho S235) antibody [EPR2452] (Anti-Tau (phospho S235) antibody [EPR2452] ab133253) at 1/1000 dilution
Lane 1: SH SY5Y cell lysates at 10 µg
Lane 2: SH SY5Y cell lysates treated with Okadaic acid and Calyculin A. at 10 µg
Predicted band size: 78 kDa
![Western blot - Anti-Tau (phospho S235) antibody [EPR2452] - BSA and Azide free (ab244235), expandable thumbnail](https://content.abcam.com/products/images/tau-phospho-s235-antibody-epr2452-bsa-and-azide-free-ab244235--western-blot-img445969.jpg "Western blot - Anti-Tau (phospho S235) antibody [EPR2452] - BSA and Azide free (ab244235)")
Western blot - Anti-Tau (phospho S235) antibody [EPR2452] - BSA and Azide free (ab244235)
Tau (phospho S235) Western blot staining using rabbit Anti-Tau (phospho S235) antibody
This data was developed using Anti-Tau (phospho S235) antibody [EPR2452] ab133253, the same antibody clone in a different buffer formulation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
Anti-Tau antibody [EP2456Y] (Anti-Tau antibody [EP2456Y] ab76128) - Total Protein Control (Anti-Tau antibody [EP2456Y] ab76128) at 1/1000 dilution.
All lanes: Western blot - Anti-Tau (phospho S235) antibody [EPR2452] (Anti-Tau (phospho S235) antibody [EPR2452] ab133253) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate (untreated membrane) at 20 µg
Lane 2: Rat brain tissue lysate (untreated membrane) at 20 µg
Lane 3: Mouse brain tissue lysate (alkaline phosphatase treated membrane) at 20 µg
Lane 4: Rat brain tissue lysate (alkaline phosphatase treated membrane) at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 35-80 kDa
Exposure time: 180s
![Indirect ELISA - Anti-Tau (phospho S235) antibody [EPR2452] - BSA and Azide free (ab244235), expandable thumbnail](https://content.abcam.com/products/images/tau-phospho-s235-antibody-epr2452-bsa-and-azide-free-ab244235--indirect-elisa-img445970.png "Indirect ELISA - Anti-Tau (phospho S235) antibody [EPR2452] - BSA and Azide free (ab244235)")
Indirect ELISA - Anti-Tau (phospho S235) antibody [EPR2452] - BSA and Azide free (ab244235)
This data was developed using Anti-Tau (phospho S235) antibody [EPR2452] ab133253, the same antibody clone in a different buffer formulation.
Indirect ELISA analysis of Anti-Tau (phospho S235) antibody [EPR2452] ab133253 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution.
Antigen: Human Tau (phospho S235) peptide,Human Tau non-phospho peptide
Antigen concentration: 1000 ng/ml.
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