AB131354

Anti-Tau (phospho S262) antibody

Name: Anti-Tau (phospho S262) antibody (ab131354) | Abcam
Brand: Abcam Limited
SKU: AB131354
Price: 430 USD
Availability: InStock
Rating: 3 (2 reviews)

(2 Reviews)

(16 Publications)

Rabbit Polyclonal TAU phospho S262 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 16 publications.

View Alternative Names

MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S262) antibody (AB131354)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S262) antibody (AB131354)

Immunohistochemical analysis of paraffin embedded Rat hippocampal region tissue from a model with Alzheimer's disease labelling Tau (phospho S262) with ab131354 antibody at a dilution of 1/50.

Immunocytochemistry/ Immunofluorescence - Anti-Tau (phospho S262) antibody (AB131354)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Tau (phospho S262) antibody (AB131354)

Immunofluorescence of methanol-fixed HeLa cells (ab29545) staining Tau (phospho S262) with ab131354 antibody at a dilution of 1/100.

Unknown

Western blot - Anti-Tau (phospho S262) antibody (AB131354)

All lanes:

Western blot - Anti-Tau (phospho S262) antibody (ab131354) at 1/500 dilution

Lane 1:

Mouse brain tissue extract with immunizing peptide

Lane 2:

Mouse brain tissue extract

Predicted band size: 78 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The specificity of this antibody refers to P10636-8.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/200", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab131354 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.88% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.

Biological function summary

Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.

Pathways

Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.

Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The protein expressed by the MAPT gene promotes microtubule assembly and stability and might be involved in establishing and maintaining neuronal polarity. Its C-terminus binds axonal microtubules, and the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between the two. Axonal polarity is predetermined by MAPT localization within the neuronal cell's domain defined by the centrosome. The short isoforms allow cytoskeleton plasticity, whereas the longer isoforms may preferentially play a role in its stabilization. This supplementary information is collated from multiple sources and compiled automatically.

See full target information MAPT phospho S262

Additional targets

MAPT phospho S262

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Publications (16)

Recent publications for all applications. Explore the full list and refine your search

The journal of trauma and acute care surgery 93:30-37 PubMed35319541

2022

Effects of antifibrinolytics on systemic and cerebral inflammation after traumatic brain injury.

Applications

Unspecified application

Species

Unspecified reactive species

Taylor E Wallen,Kathleen E Singer,Matthew R Baucom,Lisa G England,Rebecca M Schuster,Timothy A Pritts,Michael D Goodman

Biomedicines 10: PubMed35203488

2022

Relationship between Cognitive Dysfunction and Age-Related Variability in Oxidative Markers in Isolated Mitochondria of Alzheimer's Disease Transgenic Mouse Brains.

Applications

Unspecified application

Species

Unspecified reactive species

Naoki Yoshida,Yugo Kato,Hirokatsu Takatsu,Koji Fukui

Acta neuropathologica communications 9:201 PubMed34961556

2021

Emergence of distinct and heterogeneous strains of amyloid beta with advanced Alzheimer's disease pathology in Down syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Alison M Maxwell,Peng Yuan,Brianna M Rivera,Wilder Schaaf,Mihovil Mladinov,Vee P Prasher,Andrew C Robinson,William F DeGrado,Carlo Condello

Journal of Alzheimer's disease : JAD 83:1691-1702 PubMed34420960

2021

Autophagy Induction and Accumulation of Phosphorylated Tau in the Hippocampus and Prefrontal Cortex of Adult C57BL/6 Mice Subjected to Adolescent Fluoxetine Treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Jorge A Sierra-Fonseca,Minerva Rodriguez,Anapaula Themann,Omar Lira,Francisco J Flores-Ramirez,Javier Vargas-Medrano,Bharathi S Gadad,Sergio D Iñiguez

iScience 24:102993 PubMed34505007

2021

Vascular smooth muscle cell dysfunction contribute to neuroinflammation and Tau hyperphosphorylation in Alzheimer disease.

Applications

Unspecified application

Species

Unspecified reactive species

Jorge A Aguilar-Pineda,Karin J Vera-Lopez,Pallavi Shrivastava,Miguel A Chávez-Fumagalli,Rita Nieto-Montesinos,Karla L Alvarez-Fernandez,Luis D Goyzueta Mamani,Gonzalo Davila Del-Carpio,Badhin Gomez-Valdez,Clint L Miller,Rajeev Malhotra,Mark E Lindsay,Christian L Lino Cardenas

Frontiers in aging neuroscience 13:513605 PubMed33967735

2021

DHCR24 Knock-Down Induced Tau Hyperphosphorylation at Thr181, Ser199, Thr231, Ser262, Ser396 Epitopes and Inhibition of Autophagy by Overactivation of GSK3β/mTOR Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojing Bai,Junfeng Wu,Mengqi Zhang,Yixuan Xu,Lijie Duan,Kai Yao,Jianfeng Zhang,Jimei Bo,Yongfei Zhao,Guoxiong Xu,Hengbing Zu

Toxicology research 10:325-333 PubMed33884182

2021

Risk of neurodegenerative disease due to tau phosphorylation changes and arsenic exposure via drinking water.

Applications

Unspecified application

Species

Unspecified reactive species

Davoud Pakzad,Vajihe Akbari,Mohammad Reza Sepand,Mehdi Aliomrani

Neural regeneration research 16:916-923 PubMed33229729

2020

Preventive electroacupuncture reduces cognitive deficits in a rat model of D-galactose-induced aging.

Applications

Unspecified application

Species

Unspecified reactive species

Chao-Chao Yu,Chuan He,Yan-Jun Du,Shan Gao,Yuan-Fang Lin,Shu-Qin Wang,Li Wang,Jia Wang,Xue-Song Wang,Tao Jiang,Li-Hong Kong

The Journal of surgical research 259:296-304 PubMed33131764

2020

The Role of Acid Sphingomyelinase Inhibition in Repetitive Mild Traumatic Brain Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Grace M Niziolek,Richard S Hoehn,Aaron P Seitz,Peter L Jernigan,Amy T Makley,Erich Gulbins,Michael J Edwards,Michael D Goodman

Journal of neurochemistry 152:122-135 PubMed31325178

2019

High specificity of widely used phospho-tau antibodies validated using a quantitative whole-cell based assay.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Li,Yong Ku Cho

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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