Anti-Tau (phospho S293) antibody [EPR2455(2)] - BSA and Azide free

• WB

Lab

Western blot - Anti-Tau (phospho S293) antibody [EPR2455(2)] - BSA and Azide free (AB234038)

Blocking buffer and concentration : 5% NFDM/TBST Diluting buffer and concentration : 5% NFDM/TBST The molecular weight observed is consistent with what has been described in the literature (PMID : 28382304, 32692785 and 30120733). This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide ab192030.

Lanes 1 - 3:

Western blot - Anti-Tau (phospho S293) antibody [EPR2455(2)] - BSA and Azide free (ab234038) at 1/1000 dilution

Lanes 1 - 3:

Western blot - Anti-Tau (phospho S293) antibody [EPR2455(2)] (<a href='/en-us/products/primary-antibodies/tau-phospho-s293-antibody-epr24552-ab192030'>ab192030</a>) at 1/1000 dilution

Lane 1:

Untreated SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate at 15 µg

Lane 2:

SH-SY5Y treated with 1μM okadaic acid and 200nM calyculin A for 60 minutes whole cell lysate at 15 µg

Lane 3:

SH-SY5Y treated with 1μM okadaic acid and 200nM calyculin A for 60 minutes whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 78 kDa

Observed band size: 32-72 kDa

false

Exposure time: 3s

• Dot

Lab

Dot Blot - Anti-Tau (phospho S293) antibody [EPR2455(2)] - BSA and Azide free (AB234038)

Dot blot analysis of MAP4 non-phospho peptide (lane 1), Tau non-phospho peptide (lane 2), Tau S293 phospho peptide (lane 3), Tau S606 phospho peptide (lane 4), and Tau S293+S606 phospho peptide (lane 5) with ab192030 at a dilution of 1.0μg/ml. ab97051 Peroxidase conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/100,000.

Blocking buffer and concentration : 5% NFDM/TBST

Diluting buffer and concentration : 5% NFDM/TBST

Exposure time : 3 minutes

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide ab192030.