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AB196363

Anti-Tau (phospho S324) antibody [EPR2457(2)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal TAU phospho S324 antibody. Carrier free. Suitable for WB, ICC/IF, I-ELISA and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

View Alternative Names

MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau, MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-Tau (phospho S324) antibody [EPR2457(2)] - BSA and Azide free (AB196363)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Tau (phospho S324) antibody [EPR2457(2)] - BSA and Azide free (AB196363)

Immunofluorescent staining of Tau in SH-SY5Y cells using ab109401 at 1/100.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109401).

Indirect ELISA - Anti-Tau (phospho S324) antibody [EPR2457(2)] - BSA and Azide free (AB196363)
  • I-ELISA

Lab

Indirect ELISA - Anti-Tau (phospho S324) antibody [EPR2457(2)] - BSA and Azide free (AB196363)

This data was developed using ab109401, the same antibody clone in a different buffer formulation.

Indirect ELISA analysis of ab109401 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1 : 2500 dilution dilution.

Antigen : Human Tau (phospho S324) peptide, Human Tau non-phospho peptide.

Antigen concentration : 100 ng/ml

Western blot - Anti-Tau (phospho S324) antibody [EPR2457(2)] - BSA and Azide free (AB196363)
  • WB

Lab

Western blot - Anti-Tau (phospho S324) antibody [EPR2457(2)] - BSA and Azide free (AB196363)

This data was developed using ab109401, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-Tau antibody [EP2456Y] (ab76128) Total Protein Control at 1/1000 dilution.

All lanes:

Western blot - Anti-Tau (phospho S324) antibody [EPR2457(2)] (<a href='/en-us/products/primary-antibodies/tau-phospho-s324-antibody-epr24572-ab109401'>ab109401</a>) at 1/1000 dilution

Lane 1:

Untreated rat brain tissue lysate (untreated membrane) at 20 µg

Lane 2:

Rat brain treated with 1μM Okadaic Acid and 200nM Calyculin A for 1 hour, tissue lysate (untreated membrane) at 20 µg

Lane 3:

Untreated rat brain tissue lysate (alkaline phosphatase treated membrane) at 20 µg

Lane 4:

Rat brain treated with 1μM Okadaic Acid and 200nM Calyculin A for 1 hour, tissue lysate (alkaline phosphatase treated membrane) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 48-60 kDa

false

Exposure time: 70s

Western blot - Anti-Tau (phospho S324) antibody [EPR2457(2)] - BSA and Azide free (AB196363)
  • WB

Lab

Western blot - Anti-Tau (phospho S324) antibody [EPR2457(2)] - BSA and Azide free (AB196363)

This data was developed using ab109401, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-Tau antibody [EP2456Y] (ab76128) Total Protein Control at 1/1000 dilution.

All lanes:

Western blot - Anti-Tau (phospho S324) antibody [EPR2457(2)] (<a href='/en-us/products/primary-antibodies/tau-phospho-s324-antibody-epr24572-ab109401'>ab109401</a>) at 1/1000 dilution

Lane 1:

Untreated mouse brain tissue lysate (untreated membrane) at 20 µg

Lane 2:

Mouse brain treated with 1μM Okadaic Acid and 200nM Calyculin A for 1 hour, tissue lysate (untreated membrane) at 20 µg

Lane 3:

Untreated mouse brain tissue lysate (alkaline phosphatase treated membrane) at 20 µg

Lane 4:

Mouse brain treated with 1μM Okadaic Acid and 200nM Calyculin A for 1 hour, tissue lysate (alkaline phosphatase treated membrane) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 48-60 kDa

false

Exposure time: 136s

  • Unconjugated

    Anti-Tau (phospho S324) antibody [EPR2457(2)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2457(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

ICC/IF, WB, I-ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The specificity of this antibody refers to P10636-8.

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Reactivity data

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Product details

ab196363 is the carrier-free version of ab109401.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.
Biological function summary

Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.

Pathways

Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.

Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed : 21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed : 21985311, PubMed : 32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
See full target information MAPT phospho S324

Additional targets

MAPT phospho S324
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PLoS biology 23:e3002881 PubMed40163536

2025

The Hunchback transcription factor determines interneuron molecular identity, morphology, and presynapse targeting in the Drosophila NB5-2 lineage.

Applications

Unspecified application

Species

Unspecified reactive species

Heather Q Pollington,Chris Q Doe
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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