AB51036

Anti-Tau (phospho Ser673) antibody

Name: Anti-Tau (phospho Ser673) antibody (ab51036) | Abcam
Brand: Abcam Limited
SKU: AB51036
Price: 525 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal TAU phospho S673 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human MAPT phospho S673 aa 600-700.

View Alternative Names

MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho Ser673) antibody (AB51036)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho Ser673) antibody (AB51036)

Immunohistochemistry analysis using ab51036 at a dilution of 1/50 - 1/100 and human brain tissue. Right hand image : tissue treated with immunising peptide; left hand image : untreated.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human MAPT phospho S673 aa 600-700. The exact immunogen used to generate this antibody is proprietary information.

P10636

Specificity

Tau S673 was historically referred as Tau S356 in some Tau isoforms such as tau-4.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Storage buffer

pH: 7 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.

Biological function summary

Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.

Pathways

Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.

Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The protein expressed by the MAPT gene promotes microtubule assembly and stability and might be involved in establishing and maintaining neuronal polarity. Its C-terminus binds axonal microtubules, and the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between the two. Axonal polarity is predetermined by MAPT localization within the neuronal cell's domain defined by the centrosome. The short isoforms allow cytoskeleton plasticity, whereas the longer isoforms may preferentially play a role in its stabilization. This supplementary information is collated from multiple sources and compiled automatically.

See full target information MAPT phospho S673

Additional targets

MAPT phospho S356

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Acta neuropathologica communications 7:192 PubMed31796124

2019

A post-translational modification signature defines changes in soluble tau correlating with oligomerization in early stage Alzheimer's disease brain.

Applications

Unspecified application

Species

Unspecified reactive species

Ebru Ercan-Herbst,Jens Ehrig,David C Schöndorf,Annika Behrendt,Bernd Klaus,Borja Gomez Ramos,Nuria Prat Oriol,Christian Weber,Dagmar E Ehrnhoefer

Molecular neurodegeneration 12:87 PubMed29157277

2017

A validated antibody panel for the characterization of tau post-translational modifications.

Applications

Unspecified application

Species

Unspecified reactive species

Ebru Ercan,Sameh Eid,Christian Weber,Alexandra Kowalski,Maria Bichmann,Annika Behrendt,Frank Matthes,Sybille Krauss,Peter Reinhardt,Simone Fulle,Dagmar E Ehrnhoefer

Aging cell 9:174-90 PubMed20409077

2010

Altered longevity-assurance activity of p53:p44 in the mouse causes memory loss, neurodegeneration and premature death.

Applications

IHC-P

Species

Mouse

Mariana Pehar,Kenneth J O'Riordan,Melissa Burns-Cusato,Matthew E Andrzejewski,Carlos Gil del Alcazar,Corinna Burger,Heidi Scrable,Luigi Puglielli

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