AB75603

Anti-Tau (Phospho Ser673) Antibody

Name: Anti-Tau (phospho S356) antibody. Rabbit polyclonal (ab75603) | Abcam
Brand: Abcam Limited
SKU: AB75603
Price: 585 USD
Availability: InStock

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(17 Publications)

Rabbit Polyclonal TAU phospho S356 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 17 publications. Immunogen corresponding to Synthetic Peptide within Human MAPT phospho S356.

View Alternative Names

MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Tau (Phospho Ser673) Antibody (AB75603)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Tau (Phospho Ser673) Antibody (AB75603)

ab75603 at 1/100 dilution staining Tau (phospho S356) in human Hela cells by ICC/IF.

Supplier Data

Western blot - Anti-Tau (Phospho Ser673) Antibody (AB75603)

All lanes:

Western blot - Anti-Tau (Phospho Ser673) Antibody (ab75603) at 1/500 dilution

Lane 1:

Mouse brain tissue with Blocking peptide

Lane 2:

Mouse brain tissue

Predicted band size: 78 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human, Rat

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human MAPT phospho S356. The exact immunogen used to generate this antibody is proprietary information.

P10636

Specificity

The specificity of this antibody refers to P10636-8.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/200", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/100 - 1/200", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab75603 was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.

Biological function summary

Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.

Pathways

Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.

Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The protein expressed by the MAPT gene promotes microtubule assembly and stability and might be involved in establishing and maintaining neuronal polarity. Its C-terminus binds axonal microtubules, and the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between the two. Axonal polarity is predetermined by MAPT localization within the neuronal cell's domain defined by the centrosome. The short isoforms allow cytoskeleton plasticity, whereas the longer isoforms may preferentially play a role in its stabilization. This supplementary information is collated from multiple sources and compiled automatically.

See full target information MAPT phospho S356

Additional targets

MAPT phospho S356

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Publications (17)

Recent publications for all applications. Explore the full list and refine your search

Alzheimer's research & therapy 17:24 PubMed39827356

2025

Targeting early tau pathology: probiotic diet enhances cognitive function and reduces inflammation in a preclinical Alzheimer's model.

Applications

Unspecified application

Species

Unspecified reactive species

Cassandra M Flynn,Tamunotonye Omoluabi,Alyssa M Janes,Emma J Rodgers,Sarah E Torraville,Brenda L Negandhi,Timothy E Nobel,Shyamchand Mayengbam,Qi Yuan

Aging cell 24:e14405 PubMed39520141

2024

Locus coeruleus vulnerability to tau hyperphosphorylation in a rat model.

Applications

Unspecified application

Species

Unspecified reactive species

Tamunotonye Omoluabi,Zia Hasan,Jessie E Piche,Abeni R S Flynn,Jules J E Doré,Susan G Walling,Andrew C W Weeks,Touati Benoukraf,Qi Yuan

Brain communications 6:fcae136 PubMed38712317

2024

ablation attenuates pathological phenotypes in a mouse model of tauopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Grigorii Sultanakhmetov,Sophia Jobien M Limlingan,Aoi Fukuchi,Keisuke Tsuda,Hirokazu Suzuki,Iori Kato,Taro Saito,Adam Z Weitemier,Kanae Ando

Brain communications 6:fcae096 PubMed38562310

2024

Hippocampal hyperphosphorylated tau-induced deficiency is rescued by L-type calcium channel blockade.

Applications

Unspecified application

Species

Unspecified reactive species

Chelsea A Crossley,Tamunotonye Omoluabi,Sarah E Torraville,Sarah Duraid,Aida Maziar,Zia Hasan,Vishaal Rajani,Kanae Ando,Johannes W Hell,Qi Yuan

Acta neuropathologica 147:7 PubMed38175261

2024

p-tau Ser356 is associated with Alzheimer's disease pathology and is lowered in brain slice cultures using the NUAK inhibitor WZ4003.

Applications

Unspecified application

Species

Unspecified reactive species

Lewis W Taylor,Elizabeth M Simzer,Claire Pimblett,Oscar T T Lacey-Solymar,Robert I McGeachan,Soraya Meftah,Jamie L Rose,Maxwell P Spires-Jones,Kristján Holt,James H Catterson,Henner Koch,Imran Liaquat,Jonathan H Clarke,John Skidmore,Colin Smith,Sam A Booker,Paul M Brennan,Tara L Spires-Jones,Claire S Durrant

Scientific reports 13:16770 PubMed37798424

2023

Long term administration of loquat leaves and their major component, ursolic acid, attenuated endogenous amyloid-β burden and memory impairment.

Applications

Unspecified application

Species

Unspecified reactive species

Kensuke Iwasa,Sosuke Yagishita,Nan Yagishita-Kyo,Anzu Yamagishi,Shinji Yamamoto,Kota Yamashina,Chikara Haruta,Masashi Asai,Kei Maruyama,Kuniyoshi Shimizu,Keisuke Yoshikawa

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2302035 PubMed37594721

2023

Self-Aggregating Tau Fragments Recapitulate Pathologic Phenotypes and Neurotoxicity of Alzheimer's Disease in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ly Thi Huong Luu Le,Jeeyoung Lee,Dongjoon Im,Sunha Park,Kyoung-Doo Hwang,Jung Hoon Lee,Yanxialei Jiang,Yong-Seok Lee,Young Ho Suh,Hugh I Kim,Min Jae Lee

Cell reports 40:111249 PubMed36001963

2022

Disruption of nuclear envelope integrity as a possible initiating event in tauopathies.

Applications

Unspecified application

Species

Unspecified reactive species

Marine Prissette,Wen Fury,Matthew Koss,Claudia Racioppi,Daria Fedorova,Ella Dragileva,Georgia Clarke,Taylor Pohl,John Dugan,Diana Ahrens,Joyce Chiu,Charleen Hunt,Chia-Jen Siao,Tara Young,Arijit Bhowmick,Vitaliy Rogulin,Mathieu Desclaux,Eric Y Hayden,Michael Podgorski,Min Gao,Lynn E Macdonald,David Frendewey,George D Yancopoulos,Brian Zambrowicz

ACS chemical neuroscience 13:1296-1314 PubMed35357812

2022

O-GlcNAcase Inhibitor ASN90 is a Multimodal Drug Candidate for Tau and α-Synuclein Proteinopathies.

Applications

Unspecified application

Species

Unspecified reactive species

Bruno Permanne,Astrid Sand,Solenne Ousson,Maud Nény,Jennifer Hantson,Ryan Schubert,Christoph Wiessner,Anna Quattropani,Dirk Beher

Frontiers in cell and developmental biology 8:565020 PubMed33015060

2020

Upregulation of Prickle2 Ameliorates Alzheimer's Disease-Like Pathology in a Transgenic Mouse Model of Alzheimer's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Fengxian Sun,Fang Jiang,Na Zhang,Hua Li,Weiping Tian,Weiying Liu

View all publications

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