AB92682

Anti-Tau (phospho Ser673) antibody [EPR2603]

20ul selling size
RabMAb
Recombinant
What is this?

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(7 Publications)

Rabbit Recombinant Monoclonal TAU phospho S673 antibody. Suitable for WB and reacts with Human samples. Cited in 7 publications.

View Alternative Names

MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

1 Images

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Western blot - Anti-Tau (phospho Ser673) antibody [EPR2603] (AB92682)

Tau assembles into oligomers as described in the literature (PMID : 28382304, 32692785, 30120733).

All lanes:

Western blot - Anti-Tau (phospho Ser673) antibody [EPR2603] (ab92682) at 1/1000 dilution

Lane 1:

Untreated SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate at 15 µg

Lane 2:

SH-SY5Y (Human neuroblastoma epithelial cell) treated with 1uM Okadaic Acid and 200nM Calyculin A for 1 hour whole cell lysate at 15 µg

Lane 3:

SH-SY5Y (Human neuroblastoma epithelial cell) treated with 1uM Okadaic Acid and 200nM Calyculin A for 1 hour whole cell lysate, and then the membrane treated with Alkaline Phosphatase for 1 hour at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 78 kDa

Observed band size: 50-70 kDa

false

Carrier free

Anti-Tau (phospho Ser673) antibody [EPR2603] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2603

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Tau S673 was historically referred as Tau S356 in some Tau isoforms such as tau-4.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/10000", "WB-species-notes": "<p>For unpurified use at : 1:2000-1:10000</p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.

Biological function summary

Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.

Pathways

Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.

Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed : 21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed : 21985311, PubMed : 32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

See full target information MAPT phospho S673

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Genes to cells : devoted to molecular & cellular mechanisms 29:337-346 PubMed38329182

2024

Microtubule-affinity regulating kinase family members distinctively affect tau phosphorylation and promote its toxicity in a Drosophila model.

Applications

Unspecified application

Species

Unspecified reactive species

Grigorii Sultanakhmetov,Iori Kato,Akiko Asada,Taro Saito,Kanae Ando

Molecular neurobiology 61:5129-5141 PubMed38167971

2024

Palmitic Acid Induces Posttranslational Modifications of Tau Protein in Alzheimer's Disease-Related Epitopes and Increases Intraneuronal Tau Levels.

Applications

Unspecified application

Species

Unspecified reactive species

Valeria Melissa García-Cruz,Clorinda Arias

Cell reports 40:111249 PubMed36001963

2022

Disruption of nuclear envelope integrity as a possible initiating event in tauopathies.

Applications

Unspecified application

Species

Unspecified reactive species

Marine Prissette,Wen Fury,Matthew Koss,Claudia Racioppi,Daria Fedorova,Ella Dragileva,Georgia Clarke,Taylor Pohl,John Dugan,Diana Ahrens,Joyce Chiu,Charleen Hunt,Chia-Jen Siao,Tara Young,Arijit Bhowmick,Vitaliy Rogulin,Mathieu Desclaux,Eric Y Hayden,Michael Podgorski,Min Gao,Lynn E Macdonald,David Frendewey,George D Yancopoulos,Brian Zambrowicz

Oxidative medicine and cellular longevity 2022:3504279 PubMed36062186

2022

Astrocytic IGF-1 and IGF-1R Orchestrate Mitophagy in Traumatic Brain Injury via Exosomal miR-let-7e.

Applications

Unspecified application

Species

Unspecified reactive species

Ren Dabin,Chen Wei,Shu Liang,Cao Ke,Wang Zhihan,Zheng Ping

Frontiers in cellular neuroscience 13:239 PubMed31263400

2019

Activated PPARγ Abrogates Misprocessing of Amyloid Precursor Protein, Tau Missorting and Synaptotoxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Susanne Moosecker,Patrícia Gomes,Chrysoula Dioli,Shuang Yu,Ioannis Sotiropoulos,Osborne F X Almeida

Biology open 6:1013-1023 PubMed28711868

2017

Human TTBK1, TTBK2 and MARK1 kinase toxicity in is exacerbated by co-expression of human Tau.

Applications

Species

Unspecified reactive species

Josefin Fernius,Annika Starkenberg,Malgorzata Pokrzywa,Stefan Thor

Neuron 92:407-418 PubMed27720485

2016

Reduction of Nuak1 Decreases Tau and Reverses Phenotypes in a Tauopathy Mouse Model.

Applications

Species

Unspecified reactive species

Cristian A Lasagna-Reeves,Maria de Haro,Shuang Hao,Jeehye Park,Maxime W C Rousseaux,Ismael Al-Ramahi,Paymaan Jafar-Nejad,Luis Vilanova-Velez,Lauren See,Antonia De Maio,Larissa Nitschke,Zhenyu Wu,Juan C Troncoso,Thomas F Westbrook,Jianrong Tang,Juan Botas,Huda Y Zoghbi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Tau (phospho Ser673) antibody [EPR2603]

Western blot - Anti-Tau (phospho Ser673) antibody [EPR2603] (AB92682)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise