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Mouse Recombinant Monoclonal TAU phospho S396 antibody. Carrier free. Suitable for WB, Dot, IHC-P and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-Tau (phospho S396) antibody [E178] - Mouse IgG2a (Chimeric) - BSA and Azide free (AB302491), expandable thumbnail
  • Dot Blot - Anti-Tau (phospho S396) antibody [E178] - Mouse IgG2a (Chimeric) - BSA and Azide free (AB302491), expandable thumbnail
  • Western blot - Anti-Tau (phospho S396) antibody [E178] - Mouse IgG2a (Chimeric) - BSA and Azide free (AB302491), expandable thumbnail
  • Western blot - Anti-Tau (phospho S396) antibody [E178] - Mouse IgG2a (Chimeric) - BSA and Azide free (AB302491), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S396) antibody [E178] - Mouse IgG2a (Chimeric) - BSA and Azide free (AB302491), expandable thumbnail

Key facts

Isotype
IgG2a
Host species
Mouse
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBDotIHC-PIHC-Fr
Human
Tested
Tested
Tested
Not recommended
Mouse
Tested
Expected
Not recommended
Not recommended
Rat
Tested
Expected
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/20000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Target data

Function

The protein expressed by the MAPT gene promotes microtubule assembly and stability and might be involved in establishing and maintaining neuronal polarity. Its C-terminus binds axonal microtubules, and the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between the two. Axonal polarity is predetermined by MAPT localization within the neuronal cell's domain defined by the centrosome. The short isoforms allow cytoskeleton plasticity, whereas the longer isoforms may preferentially play a role in its stabilization. This supplementary information is collated from multiple sources and compiled automatically.

Alternative names

MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

Recommended products

Mouse Recombinant Monoclonal TAU phospho S396 antibody. Carrier free. Suitable for WB, Dot, IHC-P and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG2a
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
E178
Purification technique
Affinity purification Protein A
Specificity

Unsuitable for mouse and rat IHC-P and IHC-Fr.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab302491 is a mouse monoclonal chimeric antibody.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.

Biological function summary

Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.

Pathways

Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.

Associated diseases and disorders

Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-Tau (phospho S396) antibody [E178] - Mouse IgG2a (Chimeric) - BSA and Azide free (ab302491), expandable thumbnail

    Western blot - Anti-Tau (phospho S396) antibody [E178] - Mouse IgG2a (Chimeric) - BSA and Azide free (ab302491)

    Tau (phospho S396) Western blot staining using mouse Anti-Tau (phospho S396) antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Tau assembles into oligomers as described in PMID: 28382304, 32692785 and 30120733.

    Exposure time: 26 seconds.

    All lanes: Western blot - Anti-Tau (phospho S396) antibody [E178] - Mouse IgG2a (Chimeric) - BSA and Azide free (ab302491) at 1/1000 dilution

    Lane 1: SH-SY5Y (human neuroblastoma epithelial cell) whole lysate (untreated membrane) at 10 µg

    Lane 2: SH-SY5Y whole lysate (phosphatase treated membrane) at 10 µg

    Secondary

    All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L)

    Observed band size: 50-60, 100-260 kDa

  • Dot Blot - Anti-Tau (phospho S396) antibody [E178] - Mouse IgG2a (Chimeric) - BSA and Azide free (ab302491), expandable thumbnail

    Dot Blot - Anti-Tau (phospho S396) antibody [E178] - Mouse IgG2a (Chimeric) - BSA and Azide free (ab302491)

    Dot blot analysis of Tau (phospho S396) using ab302491 at 1:1000 (1.013 ug/ml) followed by a Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1:5000 dilution.

    Lane 1: Human Tau (phospho S396) phospho peptide
    Lane 2: Human Tau non-phospho peptide

    Exposure time: 3 minutes.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  • Western blot - Anti-Tau (phospho S396) antibody [E178] - Mouse IgG2a (Chimeric) - BSA and Azide free (ab302491), expandable thumbnail

    Western blot - Anti-Tau (phospho S396) antibody [E178] - Mouse IgG2a (Chimeric) - BSA and Azide free (ab302491)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    In Western blot, anti-Tau antibody (Anti-Tau antibody [EPR22524-95] ab254256) loading control staining at 1/1000 dilution.

    Exposure time: 3.25 seconds.

    All lanes: Western blot - Anti-Tau (phospho S396) antibody [E178] - Mouse IgG2a (Chimeric) - BSA and Azide free (ab302491) at 1/1000 dilution

    Lane 1: Mouse brain tissue lysate (untreated membrane) at 10 µg

    Lane 2: Mouse brain tissue lysate (phosphatase treated membrane) at 10 µg

    Secondary

    All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

    Observed band size: 50-70 kDa

  • Western blot - Anti-Tau (phospho S396) antibody [E178] - Mouse IgG2a (Chimeric) - BSA and Azide free (ab302491), expandable thumbnail

    Western blot - Anti-Tau (phospho S396) antibody [E178] - Mouse IgG2a (Chimeric) - BSA and Azide free (ab302491)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Tau assembles into oligomers as described in PMID: 28382304, 32692785 and 30120733.

    In Western blot, anti-Tau antibody (Anti-Tau antibody [EPR22524-95] ab254256) loading control staining at 1/1000 dilution.

    Exposure time: 26 seconds.

    All lanes: Western blot - Anti-Tau (phospho S396) antibody [E178] - Mouse IgG2a (Chimeric) - BSA and Azide free (ab302491) at 1/1000 dilution

    Lane 1: Rat brain tissue lysate (untreated membrane) at 10 µg

    Lane 2: Rat brain tissue lysate (phosphatase treated membrane) at 10 µg

    Secondary

    All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

    Observed band size: 50-60, 200 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S396) antibody [E178] - Mouse IgG2a (Chimeric) - BSA and Azide free (ab302491), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S396) antibody [E178] - Mouse IgG2a (Chimeric) - BSA and Azide free (ab302491)

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Tau (phospho S396) with ab302491 at 1/20000 (0.05 ug/ml) followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879).

    Positive staining on human cerebrum without alkaline phosphatase treatment (image A). No signal was detected when tissues were treated with alkaline phosphatase (image B).

    The section was incubated with ab302491 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S396) antibody [E178] - Mouse IgG2a (Chimeric) - BSA and Azide free (ab302491), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S396) antibody [E178] - Mouse IgG2a (Chimeric) - BSA and Azide free (ab302491)

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling Tau (phospho S396) with ab302491 at 1/20000 (0.05 ug/ml) followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879).

    Positive staining on human breast carcinoma without alkaline phosphatase treatment (image A). No signal was detected when tissues were treated with alkaline phosphatase (image B).

    The section was incubated with ab302491 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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