Name: Anti-Tau (phospho S396) antibody [EPR2731]
SKU: ab109390
Rating: 4 (13 reviews)

Anti-Tau (phospho S396) antibody [EPR2731] (ab109390) is a rabbit monoclonal antibody detecting Tau in Western Blot, IP, IHC-P, IHC-Fr, Dot Blot. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency

- Over 120 publications

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Anti-Tau (phospho S396) antibody [EPR2731] - BSA and Azide free
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Alexa Fluor® 647 Anti-Tau (phospho S396) antibody [EPR2731]
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Alexa Fluor® 594 Anti-Tau (phospho S396) antibody [EPR2731]
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Alexa Fluor® 568 Anti-Tau (phospho S396) antibody [EPR2731]
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Alexa Fluor® 555 Anti-Tau (phospho S396) antibody [EPR2731]
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Alexa Fluor® 488 Anti-Tau (phospho S396) antibody [EPR2731]

Images

Immunohistochemistry (Frozen sections) - Anti-Tau (phospho S396) antibody [EPR2731] (AB109390), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	Dot	WB	ICC/IF	IHC-Fr	IHC-P
Human	Tested	Expected	Tested	Not recommended	Tested	Tested
Mouse	Expected	Expected	Tested	Not recommended	Tested	Expected
Rat	Expected	Expected	Expected	Not recommended	Tested	Expected
Synthetic peptide	Not recommended	Tested	Not recommended	Not recommended	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/10 - 1/100	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Synthetic peptide	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Synthetic peptide	Dilution info 1/1000	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/10000 - 1/50000	Notes For unpurified, use 1/10000 - 1/50000.
Species Human	Dilution info 1/10000 - 1/50000	Notes For unpurified, use 1/10000 - 1/50000.

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Synthetic peptide	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat, Human, Synthetic peptide	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/100	Notes Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)
Species Rat	Dilution info 1/100	Notes Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)
Species Human	Dilution info 1/100	Notes Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

Not recommended
Not recommended

Species	Dilution info	Notes
Species Synthetic peptide	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/4000	Notes The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Synthetic peptide	Dilution info -	Notes -

Associated Products

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15 products for Alternative Product

Target data

See full target information MAPT phospho S396

Function

The protein expressed by the MAPT gene promotes microtubule assembly and stability and might be involved in establishing and maintaining neuronal polarity. Its C-terminus binds axonal microtubules, and the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between the two. Axonal polarity is predetermined by MAPT localization within the neuronal cell's domain defined by the centrosome. The short isoforms allow cytoskeleton plasticity, whereas the longer isoforms may preferentially play a role in its stabilization. This supplementary information is collated from multiple sources and compiled automatically.

Additional Targets

MAPT phospho S396

Alternative names

MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Clone number

EPR2731

Purification technique

Affinity purification Protein A

Specificity

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

The specificity of this antibody refers to P10636-8.

Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Stable for 12 months at -20°C

Notes

Tau is a protein associated with several disease states, known collectively as tauopathies. The most well-known of these is Alzheimer's disease (AD), were tau exhibiting excessive phosphorylation, aggregating to form neurofibrillary tangles. The epitope defined by phosphorylation of S396 in tau is strongly implicated in AD-associated tau pathology, providing a valuable target for the development of therapeutic antibodies to capture tau and prevent spreading of tau pathology.

Product Specifications
Anti-Tau (phospho S396) antibody [EPR2731] (ab109390) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Dot blot, IHC-Fr, IHC-P, IP, WB in human, mouse, rat samples.
Anti-Tau (phospho S396) antibody [EPR2731] (ab109390) specifically detects Tau Phospho-S396 (UniProt ID: P10636; Molecular weight: 79kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL).

Quality and Validation
Abcam's high quality manufacturing and validation processes ensure Anti-Tau (phospho S396) antibody [EPR2731] (ab109390) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-Tau (phospho S396) antibody [EPR2731] (ab109390) has been cited over 127 times in peer reviewed journals and is trusted by the scientific community.
Anti-Tau (phospho S396) antibody [EPR2731] (ab109390) has 12 independent reviews from customers.

Related Products
Antibody clone EPR2731 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 647, Alexa Fluor® 555, Alexa Fluor® 488, Alexa Fluor® 594, Alexa Fluor® 568 (ab300748, ab302570, ab302581, ab302686, ab302804).

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

11 product images

Immunohistochemistry (Frozen sections) - Anti-Tau (phospho S396) antibody [EPR2731] (ab109390)
IHC image of Tau (phospho S396) staining in a section of frozen normal human Alzheimer brain performed on a Leica BOND^TM system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab109390, 1/1000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Image courtesy of Carl Hobbs, Kings College London, U.K.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S396) antibody [EPR2731] (ab109390)
IHC image of Tau (phospho S396) staining in human Alzheimer hippocampus formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with citrate buffer. The section was then incubated with unpurified ab109390 at 1/1000 dilution for 2 hoursat 21°C. A biotin conjugated goat-anti-rabbit antibody was used as a secondary at 1/250. The section shows clear neurofibrillary tangles in a subset of neurons.
Immunohistochemistry (Frozen sections) - Anti-Tau (phospho S396) antibody [EPR2731] (ab109390)
Immunohistochemistry analysis of frozen mouse cerebrum tissue sections labeling Tau (phospho S396) with ab109390 at 1/100 (1 μg/mL). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor^®488 Goat anti-Rabbit at 1/1000 (2 μg/mL) was used as the secondary antibody. Sections were fixed with 4% PFA and permeabilised with 0.2% Triton X-100. DAPI (blue) was used as nuclear counterstain. Antigen retrieval was heat mediated using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Cytoplasmic staining on mouse cerebrum, the signal decreased after phosphatase treatment at 37℃ for 2h.
Immunoprecipitation - Anti-Tau (phospho S396) antibody [EPR2731] (ab109390)
ab109390 at 1/20 immunoprecipitating Tau (phospho S396) in Human brain lysate.
Lane 1 (input): Human brain lysate (10μg)
Lane 2 (+): ab109390 + Human brain lysate.
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab109390 in Human brain lysate.
For western blotting, ab109390 at 1/1000 dilution followed by VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/5000 dilution.
Diluting / Blocking buffer and concentration: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-Tau (phospho S396) antibody [EPR2731] (ab109390)
Predicted band size: 78 kDa
Observed band size: 50-70 kDa
Exposure time: 3min
Western blot - Anti-Tau (phospho S396) antibody [EPR2731] (ab109390)
Tau (phospho S396) Western blot staining using rabbit Anti-Tau (phospho S396) antibody
Blocking/Diluting buffer and concentration 5% NFDM/TBST
Tau assembles into oligomers as described in PMID: 28382304, 32692785 and 30120733.
All lanes: Western blot - Anti-Tau (phospho S396) antibody [EPR2731] (ab109390) at 1/1000 dilution
Lane 1: Human brain lysate at 15 µg
Lane 2: Human brain lysates and the membrane was incubated with alkaline phosphatase at 15 µg
Lane 3: Human brain lysates and the membrane was incubated with lambda phosphatase at 15 µg
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 78 kDa
Observed band size: 50-79 kDa
Exposure time: 100s
Western blot - Anti-Tau (phospho S396) antibody [EPR2731] (ab109390)
Tau (phospho S396) Western blot staining using rabbit Anti-Tau (phospho S396) antibody
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-Tau (phospho S396) antibody [EPR2731] (ab109390) at 1/20000 dilution
Lane 1: Untreated SH-SY5Y at 10 µg
Lane 2: SH-SY5Y treated with alkaline phosphatase at 10 µg
Secondary
All lanes: HRP goat ant-rabbit (H+L) at 1/1000 dilution
Predicted band size: 78 kDa
Observed band size: 50-70 kDa
Western blot - Anti-Tau (phospho S396) antibody [EPR2731] (ab109390)
Tau (phospho S396) Western blot staining using rabbit Anti-Tau (phospho S396) antibody
Diluting/Diluting buffer and concentration 5% NFDM/TBST
Tau assembles into oligomers as described in PMID: 28382304, 32692785 and 30120733..
All lanes: Western blot - Anti-Tau (phospho S396) antibody [EPR2731] (ab109390) at 1/1000 dilution
Lane 1: Mouse brain lysate at 15 µg
Lane 2: Mouse brain lysates and the membrane was incubated with alkaline phosphatase at 15 µg
Lane 3: Mouse brain lysates and the membrane was incubated with lambda phosphatase at 15 µg
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 78 kDa
Observed band size: 50-79 kDa
Exposure time: 10s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S396) antibody [EPR2731] (ab109390)
Immunohistochemical staining of paraffin embedded human glioblastoma with purified ab109390 at a dilution of 1/4000. A pre-diluted HRP polymer for rabbit/mouse IgG was used as the secondary antibody and the sample was counter-stained wirh hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Immunohistochemistry (Frozen sections) - Anti-Tau (phospho S396) antibody [EPR2731] (ab109390)
Dot Blot - Anti-Tau (phospho S396) antibody [EPR2731] (ab109390)
Dot blot analysis of Tau (phospho S396) phospho peptide (Lane 1) and Tau non-phospho peptide (Lane 2) labeling Tau (phospho S396) with ab109390 at a dilution of 1/1000. Goat Anti-Rabbit IgG H&L (HRP) ab97051 (Peroxidase conjugated goat anti-rabbit IgG) (H+L) at 1/100 000 was used as the secondary antibody.
Blocking and diluting buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S396) antibody [EPR2731] (ab109390)
Immunohistochemistry analysis of paraffin-embedded human colon tissue sections labelling Tau (phospho S396) with ab109390 at 1/4000 dilution (0.026 μg/mL). The section was incubated with ab109390 for 30 mins at room temperature. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.
Positive staining on ganglions of human colon without alkaline phosphatase treatment (image A); No signal was detected when tissues were treated with alkaline phosphatase (image B).The immunostaining was performed on a Leica Biosystems BOND® RX instrument.