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AB79415

Anti-Tau (phospho S422) antibody [EPR2866]

2

(1 Review)

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(33 Publications)

Anti-Tau (phospho S422) antibody [EPR2866] (ab79415) is a rabbit monoclonal antibody detecting Tau in Western Blot, Dot Blot. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency
- Over 20 publications
- Trusted since 2009

View Alternative Names

MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

4 Images
Western blot - Anti-Tau (phospho S422) antibody [EPR2866] (AB79415)
  • WB

Unknown

Western blot - Anti-Tau (phospho S422) antibody [EPR2866] (AB79415)

All lanes:

Western blot - Anti-Tau (phospho S422) antibody [EPR2866] (ab79415) at 1/20000 dilution

Lane 1:

SH-SY5Y cell lysates at 10 µg

Lane 2:

SH-SY5Y cell lysates treated with Okadiac acid and calyculin A at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 78 kDa

Observed band size: 55 kDa

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Western blot - Anti-Tau (phospho S422) antibody [EPR2866] (AB79415)
  • WB

Lab

Western blot - Anti-Tau (phospho S422) antibody [EPR2866] (AB79415)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Tau (phospho S422) antibody [EPR2866] (ab79415) at 1/1000 dilution

Lane 1:

IMR-32 (human neuroblastoma neuroblast) whole cell lysate at 10 µg

Lane 2:

IMR-32 (human neuroblastoma neuroblast) whole cell lysate, then membrane was incubated with phosphatase at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 78 kDa

Observed band size: 55 kDa

false

Exposure time: 3min

Western blot - Anti-Tau (phospho S422) antibody [EPR2866] (AB79415)
  • WB

Lab

Western blot - Anti-Tau (phospho S422) antibody [EPR2866] (AB79415)

Blocking and dilution buffer : 5% NFDM/TBST.

Western blot for ab205606 on the right hand side used as a control for flag tag used.

All lanes:

Western blot - Anti-Tau (phospho S422) antibody [EPR2866] (ab79415) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a flag tag whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human Tau expression vector containing a flag whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a human MAP2 expression vector containing a flag whole cell lysate at 20 µg

Lane 4:

293T cells transfected with a human MAP4 expression vector containing a flag whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 50-100 kDa

false

Exposure time: 1s

Dot Blot - Anti-Tau (phospho S422) antibody [EPR2866] (AB79415)
  • Dot

Unknown

Dot Blot - Anti-Tau (phospho S422) antibody [EPR2866] (AB79415)

Dot blot analysis of Tau (pS422) peptide (Lane 1) and Tau non-phospho peptide (Lane 2) labelling Tau (pS422) with ab79415 at a dilution of 1/1000. ab97051 (Peroxidase-conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/100000.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

  • Carrier free

    Anti-Tau (phospho S422) antibody [EPR2866] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2866

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, Agg, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The specificity of this antibody refers to P10636-8.

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Reactivity data

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Product details

What is this antibody validated in?
Anti-Tau (phospho S422) antibody [EPR2866] (ab79415) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Dot Blot in Human samples.

What is the molecular weight of Tau?
Anti-Tau (phospho S422) [EPR2866] (ab79415) specifically detects a band for Tau (UniProt: P10636) at a molecular weight of 79kDa.

Trusted by the scientific community
Anti-Tau (phospho S422) [EPR2866] (ab79415) was first used in a scientific publication in 2009 and has been cited over 20 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR2866] also available for your convenience: ab79415, Carrier free - ab196365

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.31% Sodium citrate, 0.175% Sodium chloride, 0.05% BSA, 0.0172% Ethylenediaminetetraacetic acid, disodium, dihydrate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.
Biological function summary

Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.

Pathways

Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3Β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.

Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The protein expressed by the MAPT gene promotes microtubule assembly and stability and might be involved in establishing and maintaining neuronal polarity. Its C-terminus binds axonal microtubules, and the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between the two. Axonal polarity is predetermined by MAPT localization within the neuronal cell's domain defined by the centrosome. The short isoforms allow cytoskeleton plasticity, whereas the longer isoforms may preferentially play a role in its stabilization. This supplementary information is collated from multiple sources and compiled automatically.
See full target information MAPT phospho S422

Additional targets

MAPT phospho S422
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Publications (33)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 301:108245 PubMed39880096

2025

Sensitive detection and propagation of brain-derived tau assemblies in HEK293-based wild-type tau seeding assays.

Applications

Unspecified application

Species

Unspecified reactive species

Melissa Huang,William A McEwan

Journal of neurochemistry 169:e16221 PubMed39308063

2024

Mass spectrometry identifies tau C-terminal phosphorylation cluster during neuronal hyperexcitation.

Applications

Unspecified application

Species

Unspecified reactive species

Amanda Schneeweis,Dawson Hillyer,Tsering Lama,Daeun Kim,Charles Palka,Sarra Djemil,Mai Abdel-Ghani,Kelly Mandella,William Zhu,Nicole Alvarez,Lara Stefansson,Robert Yasuda,Junfeng Ma,Daniel T S Pak

Alzheimer's & dementia : the journal of the Alzheimer's Association 20:5198-5219 PubMed39030748

2024

Temporal progression of tau pathology and neuroinflammation in a rhesus monkey model of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Danielle Beckman,Giovanne B Diniz,Sean Ott,Brad Hobson,Abhijit J Chaudhari,Scott Muller,Yaping Chu,Akihiro Takano,Adam J Schwarz,Chien-Lin Yeh,Paul McQuade,Paramita Chakrabarty,Nicholas M Kanaan,Maria S Quinton,Arthur A Simen,Jeffrey H Kordower,John H Morrison

Communications biology 7:872 PubMed39020075

2024

Selenoprotein W modulates tau homeostasis in an Alzheimer's disease mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Bingyu Ren,Jiaxin Situ,Xuelian Huang,Qiulong Tan,Shifeng Xiao,Nan Li,Jing Tian,Xiubo Du,Jiazuan Ni,Qiong Liu

Acta neuropathologica communications 11:166 PubMed37853497

2023

Antisense oligonucleotide-based targeting of Tau-tubulin kinase 1 prevents hippocampal accumulation of phosphorylated tau in PS19 tauopathy mice.

Applications

Unspecified application

Species

Unspecified reactive species

Kayo Yukawa,Satomi Yamamoto-Mcguire,Louis Cafaro,Christine Hong,Fredrik Kamme,Tsuneya Ikezu,Seiko Ikezu

Alzheimer's & dementia : the journal of the Alzheimer's Association 20:1013-1025 PubMed37849026

2023

The type-I interferon response potentiates seeded tau aggregation and exacerbates tau pathology.

Applications

Unspecified application

Species

Unspecified reactive species

Sophie A I Sanford,Lauren V C Miller,Marina Vaysburd,Sophie Keeling,Benjamin J Tuck,Jessica Clark,Michal Neumann,Victoria Syanda,Leo C James,William A McEwan

Frontiers in aging neuroscience 15:1265151 PubMed37842124

2023

Behavioral and neuropathological characterization over the adult lifespan of the human tau knock-in mouse.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew J Benskey,Spencer Panoushek,Takashi Saito,Takaomi C Saido,Tessa Grabinski,Nicholas M Kanaan

Frontiers in cellular neuroscience 16:979652 PubMed36619675

2022

Veteran-derived cerebral organoids display multifaceted pathological defects in studies on Gulf War Illness.

Applications

Unspecified application

Species

Unspecified reactive species

Philip L Yates,Kendra Case,Xiaohuan Sun,Kimberly Sullivan,Peter W Baas,Liang Qiang

eNeuro 9: PubMed36635241

2022

Increase in Tau Pathology in P290S Knock-In Mice Crossed with Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Melissa Huang,Jennifer Macdonald,Isabelle Lavenir,Renren Chen,Molly Craxton,Elizabeth Slavik-Smith,Stephen W Davies,Michel Goedert

Science advances 8:eadd2577 PubMed36459557

2022

Treatment of epilepsy using a targeted p38γ kinase gene therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Nicolle Morey,Magdalena Przybyla,Julia van der Hoven,Yazi D Ke,Fabien Delerue,Janet van Eersel,Lars M Ittner
View all publications
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