Rabbit Polyclonal TAU phospho T181 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human MAPT phospho T181.
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- International journal of molecular sciences 24:2023Beta-Amyloid Peptide in Tears: An Early Diagnostic Marker of Alzheimer's Disease Correlated with Choroidal Thickness.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMagda Gharbiya,Giacomo Visioli,Alessandro Trebbastoni,Giuseppe Maria Albanese,Mayra Colardo,Fabrizia D'Antonio,Marco Segatto,Alessandro LambiasePubMed 36768913
- Frontiers in molecular neuroscience 15:10250662023Discovery of an evodiamine derivative for PI3K/AKT/GSK3β pathway activation and AD pathology improvement in mouse models.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuo Pang,Siyuan Li,Hanzeng Cheng,Zhuohui Luo,Xiaolong Qi,Feifei Guan,Wei Dong,Shan Gao,Ning Liu,Xiang Gao,Shuo Pan,Xu Zhang,Li Zhang,Yajun Yang,Lianfeng ZhangPubMed 36698780
- Science advances 8:eabl88092022Alzheimer's disease: Ablating single master site abolishes tau hyperphosphorylation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKristie Stefanoska,Mehul Gajwani,Amanda R P Tan,Holly I Ahel,Prita R Asih,Alexander Volkerling,Anne Poljak,Arne IttnerPubMed 35857446
- Frontiers in aging neuroscience 13:7664102022Agomelatine Prevents Amyloid Plaque Deposition, Tau Phosphorylation, and Neuroinflammation in APP/PS1 Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiao-Bo Yang,Heng-Bing Zu,Yong-Fei Zhao,Kai YaoPubMed 35153715
- Brain pathology (Zurich, Switzerland) 32:e130352021Novel genetic variants in MAPT and alterations in tau phosphorylation in amyotrophic lateral sclerosis post-mortem motor cortex and cerebrospinal fluid.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTiziana Petrozziello,Ana C Amaral,Simon Dujardin,Sali M K Farhan,James Chan,Bianca A Trombetta,Pia Kivisäkk,Alexandra N Mills,Evan A Bordt,Spencer E Kim,Patrick M Dooley,Caitlin Commins,Theresa R Connors,Derek H Oakley,Anubrata Ghosal,Teresa Gomez-Isla,Bradley T Hyman,Steven E Arnold,Tara Spires-Jones,Merit E Cudkowicz,James D Berry,Ghazaleh Sadri-VakiliPubMed 34779076
- Molecular brain 13:582020Nasal delivery of a CRMP2-derived CBD3 adenovirus improves cognitive function and pathology in APP/PS1 transgenic mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBaochang Qi et. al.PubMed 32272942
- Experimental cell research 370:103-1152018Pseudo-phosphorylation at AT8 epitopes regulates the tau truncation at aspartate 421.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLan Cao et. al.PubMed 29908160
- Aging 8:2713-27332016An antioxidant specifically targeting mitochondria delays progression of Alzheimer's disease-like pathology.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNatalia A Stefanova,Natalia A Muraleva,Kseniya Yi Maksimova,Ekaterina A Rudnitskaya,Elena Kiseleva,Darya V Telegina,Nataliya G KolosovaPubMed 27750209
- Journal of neuroinflammation 13:1752016Long-term trihexyphenidyl exposure alters neuroimmune response and inflammation in aging rat: relevance to age and Alzheimer's disease.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human MAPT phospho T181. The exact immunogen used to generate this antibody is proprietary information. Database link P10636
Consider this alternative
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Mouse | Tested | Expected |
| Rat | Expected | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/50 - 1/100 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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7 products for Alternative Product
Target data
Function
The protein expressed by the MAPT gene promotes microtubule assembly and stability and might be involved in establishing and maintaining neuronal polarity. Its C-terminus binds axonal microtubules, and the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between the two. Axonal polarity is predetermined by MAPT localization within the neuronal cell's domain defined by the centrosome. The short isoforms allow cytoskeleton plasticity, whereas the longer isoforms may preferentially play a role in its stabilization. This supplementary information is collated from multiple sources and compiled automatically.
Additional Targets
MAPT phospho T181
Alternative names
MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau
Recommended products
Rabbit Polyclonal TAU phospho T181 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human MAPT phospho T181.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human MAPT phospho T181. The exact immunogen used to generate this antibody is proprietary information. Database link P10636
- Purification technique
- Affinity purification Immunogen
- Specificity
ab75679 detects endogenous levels of isoform Tau-F, which is a truncated form of the unprocessed precursor isoform PNS-tau (http://www.uniprot.org/uniprot/P10636)), where the phosphorylation site is located at threonine 181.
The specificity of this antibody refers to P10636-8.
- Concentration
- Purification notes
ab75679 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.
- Biological function summary
Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.
- Pathways
Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.
- Associated diseases and disorders
Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T181) antibody (ab75679)
ab75679 at 1/50 dilution staining Tau in rat hippocampal region tissue from a model with Alzheimer’s Disease by Immunohistochemistry, Paraffin-embedded tissue.
Western blot - Anti-Tau (phospho T181) antibody (ab75679)
All lanes: Western blot - Anti-Tau (phospho T181) antibody (ab75679) at 1/500 dilution
Lane 1: mouse brain tissue extracts with immunising peptide
Lane 2: mouse brain tissue extracts
Predicted band size: 78 kDa
Observed band size: 35 kDa, 46 kDa
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-Tau (phospho T181) antibody (ab75679)
Tau (phospho T181) western blot using anti-Tau (phospho T181) antibody ab75679. Publication image and figure legend from Qi, B., Yang, Y., et al., 2020, Mol Brain, PubMed 32272942.
ab75679 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab75679 please see the product overview.
CBD3 expression reduces Aβ1–42 and p-tau plaques. Representative western blots of lysates (n = 4) from the indicated conditions probed with antibodies against ADAM10, Aβ1–42, p-tau, t-tau, or GAPDH (housekeeping/loading control). The hippocampal tissues were from wildtype (WT) mice, APP/PS1 mice, APP/PS1 mice administered control AAV, or APP/PS1 mice administered CBD3 harboring AAV. Quantification of levels of Aβ1–42 (b), p-tau/t-tau (c), and ADAM10 (d) normalized to control (n = 4 per condition). *P < 0.05, one-way ANOVA with Dunnett’s post-hoc test. n.s., not significant.
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