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AB75679

Anti-Tau (phospho T181) antibody

4

(1 Review)

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(10 Publications)

Rabbit Polyclonal TAU phospho T181 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human MAPT phospho T181.

View Alternative Names

MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T181) antibody (AB75679)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T181) antibody (AB75679)

ab75679 at 1/50 dilution staining Tau in rat hippocampal region tissue from a model with Alzheimer’s Disease by Immunohistochemistry, Paraffin-embedded tissue.

Western blot - Anti-Tau (phospho T181) antibody (AB75679)
  • WB

Unknown

Western blot - Anti-Tau (phospho T181) antibody (AB75679)

All lanes:

Western blot - Anti-Tau (phospho T181) antibody (ab75679) at 1/500 dilution

Lane 1:

mouse brain tissue extracts with immunising peptide

Lane 2:

mouse brain tissue extracts

Predicted band size: 78 kDa

Observed band size: 35 kDa,46 kDa

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Western blot - Anti-Tau (phospho T181) antibody (AB75679)
  • WB

CiteAb

Western blot - Anti-Tau (phospho T181) antibody (AB75679)

Tau (phospho T181) western blot using anti-Tau (phospho T181) antibody ab75679. Publication image and figure legend from Qi, B., Yang, Y., et al., 2020, Mol Brain, PubMed 32272942.

ab75679 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab75679 please see the product overview.

CBD3 expression reduces Aβ1–42 and p-tau plaques. Representative western blots of lysates (n = 4) from the indicated conditions probed with antibodies against ADAM10, Aβ1–42, p-tau, t-tau, or GAPDH (housekeeping/loading control). The hippocampal tissues were from wildtype (WT) mice, APP/PS1 mice, APP/PS1 mice administered control AAV, or APP/PS1 mice administered CBD3 harboring AAV. Quantification of levels of Aβ1–42 (b), p-tau/t-tau (c), and ADAM10 (d) normalized to control (n = 4 per condition). *p < 0.05, one-way ANOVA with Dunnett's post-hoc test. n.s., not significant.

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human MAPT phospho T181. The exact immunogen used to generate this antibody is proprietary information.

P10636

Specificity

ab75679 detects endogenous levels of isoform Tau-F, which is a truncated form of the unprocessed precursor isoform PNS-tau (http://www.uniprot.org/uniprot/P10636)), where the phosphorylation site is located at threonine 181.

The specificity of this antibody refers to P10636-8.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab75679 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.
Biological function summary

Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.

Pathways

Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.

Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The protein expressed by the MAPT gene promotes microtubule assembly and stability and might be involved in establishing and maintaining neuronal polarity. Its C-terminus binds axonal microtubules, and the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between the two. Axonal polarity is predetermined by MAPT localization within the neuronal cell's domain defined by the centrosome. The short isoforms allow cytoskeleton plasticity, whereas the longer isoforms may preferentially play a role in its stabilization. This supplementary information is collated from multiple sources and compiled automatically.
See full target information MAPT phospho T181

Additional targets

MAPT phospho T181
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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 7:872 PubMed39020075

2024

Selenoprotein W modulates tau homeostasis in an Alzheimer's disease mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Bingyu Ren,Jiaxin Situ,Xuelian Huang,Qiulong Tan,Shifeng Xiao,Nan Li,Jing Tian,Xiubo Du,Jiazuan Ni,Qiong Liu

International journal of molecular sciences 24: PubMed36768913

2023

Beta-Amyloid Peptide in Tears: An Early Diagnostic Marker of Alzheimer's Disease Correlated with Choroidal Thickness.

Applications

Unspecified application

Species

Unspecified reactive species

Magda Gharbiya,Giacomo Visioli,Alessandro Trebbastoni,Giuseppe Maria Albanese,Mayra Colardo,Fabrizia D'Antonio,Marco Segatto,Alessandro Lambiase

Frontiers in molecular neuroscience 15:1025066 PubMed36698780

2023

Discovery of an evodiamine derivative for PI3K/AKT/GSK3β pathway activation and AD pathology improvement in mouse models.

Applications

Unspecified application

Species

Unspecified reactive species

Shuo Pang,Siyuan Li,Hanzeng Cheng,Zhuohui Luo,Xiaolong Qi,Feifei Guan,Wei Dong,Shan Gao,Ning Liu,Xiang Gao,Shuo Pan,Xu Zhang,Li Zhang,Yajun Yang,Lianfeng Zhang

Science advances 8:eabl8809 PubMed35857446

2022

Alzheimer's disease: Ablating single master site abolishes tau hyperphosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Kristie Stefanoska,Mehul Gajwani,Amanda R P Tan,Holly I Ahel,Prita R Asih,Alexander Volkerling,Anne Poljak,Arne Ittner

Frontiers in aging neuroscience 13:766410 PubMed35153715

2022

Agomelatine Prevents Amyloid Plaque Deposition, Tau Phosphorylation, and Neuroinflammation in APP/PS1 Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Bo Yang,Heng-Bing Zu,Yong-Fei Zhao,Kai Yao

Brain pathology (Zurich, Switzerland) 32:e13035 PubMed34779076

2021

Novel genetic variants in MAPT and alterations in tau phosphorylation in amyotrophic lateral sclerosis post-mortem motor cortex and cerebrospinal fluid.

Applications

Unspecified application

Species

Unspecified reactive species

Tiziana Petrozziello,Ana C Amaral,Simon Dujardin,Sali M K Farhan,James Chan,Bianca A Trombetta,Pia Kivisäkk,Alexandra N Mills,Evan A Bordt,Spencer E Kim,Patrick M Dooley,Caitlin Commins,Theresa R Connors,Derek H Oakley,Anubrata Ghosal,Teresa Gomez-Isla,Bradley T Hyman,Steven E Arnold,Tara Spires-Jones,Merit E Cudkowicz,James D Berry,Ghazaleh Sadri-Vakili

Molecular brain 13:58 PubMed32272942

2020

Nasal delivery of a CRMP2-derived CBD3 adenovirus improves cognitive function and pathology in APP/PS1 transgenic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Baochang Qi,Yu Yang,Yingying Cheng,Di Sun,Xu Wang,Rajesh Khanna,Weina Ju

Experimental cell research 370:103-115 PubMed29908160

2018

Pseudo-phosphorylation at AT8 epitopes regulates the tau truncation at aspartate 421.

Applications

Unspecified application

Species

Unspecified reactive species

Lan Cao,Yan Liang,Yunsheng Liu,Yuxia Xu,Wenbin Wan,Cuiqing Zhu

Aging 8:2713-2733 PubMed27750209

2016

An antioxidant specifically targeting mitochondria delays progression of Alzheimer's disease-like pathology.

Applications

Unspecified application

Species

Unspecified reactive species

Natalia A Stefanova,Natalia A Muraleva,Kseniya Yi Maksimova,Ekaterina A Rudnitskaya,Elena Kiseleva,Darya V Telegina,Nataliya G Kolosova

Journal of neuroinflammation 13:175 PubMed27411393

2016

Long-term trihexyphenidyl exposure alters neuroimmune response and inflammation in aging rat: relevance to age and Alzheimer's disease.

Applications

WB

Species

Unspecified reactive species

Yuqi Huang,Zhe Zhao,Xiaoli Wei,Yong Zheng,Jianqiang Yu,Jianquan Zheng,Liyun Wang
View all publications
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