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AB232849

Anti-Tau (phospho T181) antibody [EPADXR4]

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(2 Publications)

Rabbit Recombinant Monoclonal TAU phospho T181 antibody. Suitable for ELISA, Dot and reacts with Synthetic peptide, Human samples. Cited in 2 publications.

View Alternative Names

MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau, MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

2 Images
ELISA - Anti-Tau (phospho T181) antibody [EPADXR4] (AB232849)
  • ELISA

Unknown

ELISA - Anti-Tau (phospho T181) antibody [EPADXR4] (AB232849)

ELISA - Anti-Tau (phospho T181) antibody (ab232849) used at 1 μg/ml.

Antigen used at 0-1000 ng/ml.

Alkaline Phosphatase-conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody used at a 1/2500 dilution.

Dot Blot - Anti-Tau (phospho T181) antibody [EPADXR4] (AB232849)
  • Dot

Unknown

Dot Blot - Anti-Tau (phospho T181) antibody [EPADXR4] (AB232849)

Dot Blot - Anti-Tau (phospho T181) antibody (ab232849) used at a 1/1000 dilution.

Lane 1 : Tau (phospho T181) peptide.

Lane 2 : Tau non-phospho peptide.

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody used at a 1/100,000 dilution.

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time is 3 minutes.

  • Carrier free

    Anti-Tau (phospho T181) antibody [EPADXR4] - BSA and Azide free

  • Carrier free

    Anti-Tau (phospho T181) antibody [EPADXR4] - BSA and Azide free (Capture)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPADXR4

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ELISA, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The specificity of this antibody refers to P10636-8.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "1 µg/mL", "ELISA-species-notes": "<p></p>", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Synthetic peptide": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "1 µg/mL", "ELISA-species-notes": "<p></p>", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.
Biological function summary

Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.

Pathways

Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.

Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed : 21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed : 21985311, PubMed : 32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
See full target information MAPT phospho T181

Additional targets

MAPT phospho T181
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Analytica chimica acta 1273:341535 PubMed37423666

2023

Electrochemical impedance-based biosensor for label-free determination of plasma P-tau181 levels for clinically accurate diagnosis of mild cognitive impairment and Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

My-Van Tieu,Seong Hye Choi,Hien T Ngoc Le,Sungbo Cho

Journal of cellular biochemistry 121:1552-1562 PubMed31512789

2019

Decreased SPTLC1 expression predicts worse outcomes in ccRCC patients.

Applications

Unspecified application

Species

Unspecified reactive species

Wen-Kai Zhu,Wen-Hao Xu,Jun Wang,Yong-Qiang Huang,Mierxiati Abudurexiti,Yuan-Yuan Qu,Yi-Ping Zhu,Hai-Liang Zhang,Ding-Wei Ye
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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