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AB236458

Anti-Tau (phospho T181) antibody [EPADXR4] - BSA and Azide free

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(2 Publications)

Anti-Tau (phospho T181) antibody [EPADXR4] - BSA and Azide free (ab236458) is a rabbit recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation detecting Tau in ELISA, Dot Blot. Suitable for Human.

- BSA, sodium azide, and glycerol-free for easy conjugation
- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau, MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

2 Images
ELISA - Anti-Tau (phospho T181) antibody [EPADXR4] - BSA and Azide free (AB236458)
  • ELISA

Unknown

ELISA - Anti-Tau (phospho T181) antibody [EPADXR4] - BSA and Azide free (AB236458)

ELISA - Anti-Tau (phospho T181) antibody (ab232849) used at 1 μg/ml.

Antigen used at 0-1000 ng/ml.

Alkaline Phosphatase-conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody used at a 1/2500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab232849).

Dot Blot - Anti-Tau (phospho T181) antibody [EPADXR4] - BSA and Azide free (AB236458)
  • Dot

Unknown

Dot Blot - Anti-Tau (phospho T181) antibody [EPADXR4] - BSA and Azide free (AB236458)

Dot Blot - Anti-Tau (phospho T181) antibody (ab232849) used at a 1/1000 dilution.

Lane 1 : Tau (phospho T181) peptide.

Lane 2 : Tau non-phospho peptide.

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody used at a 1/100,000 dilution.

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time is 3 minutes.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab232849).

  • Carrier free

    Anti-Tau (phospho T181) antibody [EPADXR4] - BSA and Azide free (Capture)

  • Unconjugated

    Anti-Tau (phospho T181) antibody [EPADXR4]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPADXR4

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Dot, ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The specificity of this antibody refers to P10636-8.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Synthetic peptide": { "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

What is this antibody validated in?
Anti-Tau (phospho T181) antibody [EPADXR4] - BSA and Azide free (ab236458) is a rabbit recombinant monoclonal antibody and is validated for use in ELISA, Dot Blot in Human samples.

Other related products
We have a range of other formats of antibody clone [EPADXR4] also available for your convenience: ab232849, Carrier free - ab236458

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.
Biological function summary

Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.

Pathways

Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3Β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.

Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed : 21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed : 21985311, PubMed : 32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
See full target information MAPT phospho T181

Additional targets

MAPT phospho T181
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Acta neuropathologica communications 11:181 PubMed37964332

2023

Tau seed amplification assay reveals relationship between seeding and pathological forms of tau in Alzheimer's disease brain.

Applications

Unspecified application

Species

Unspecified reactive species

Bryan Frey,David Holzinger,Keenan Taylor,Dagmar E Ehrnhoefer,Andreas Striebinger,Sandra Biesinger,Laura Gasparini,Michael J O'Neill,Florian Wegner,Stefan Barghorn,Günter U Höglinger,Roland G Heym

Frontiers in pharmacology 12:706617 PubMed34690754

2021

Polysaccharide Reduces Blood Pressure, Renal Damage, and Dysfunction Through the TGF-1-ILK Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Zheng,Tao Huang,Qi-Zhen Tang,Shi Li,Jie Qin,Feng Chen
View all publications
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Product promise

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