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AB240579

Anti-Tau (phospho T181) antibody [EPR22064] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal TAU phospho T181 antibody. Carrier free. Suitable for IP, Dot, WB, I-ELISA and reacts with Human, Synthetic peptide, Synthetic peptide - Human samples. Cited in 1 publication.

View Alternative Names

MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

4 Images
Immunoprecipitation - Anti-Tau (phospho T181) antibody [EPR22064] - BSA and Azide free (AB240579)
  • IP

Unknown

Immunoprecipitation - Anti-Tau (phospho T181) antibody [EPR22064] - BSA and Azide free (AB240579)

Tau (phospho T181 was immunoprecipitated from 0.35 mg of human brain lysate with ab223192 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab223192 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1 : Human brain lysate 10 μg (Input).
Lane 2 : ab223192 IP in Human brain lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab223192 in Human brain lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST
Exposure time : 3 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223192).

All lanes:

Immunoprecipitation - Anti-Tau (phospho T181) antibody [EPR22064] (<a href='/en-us/products/primary-antibodies/tau-phospho-t181-antibody-epr22064-ab223192'>ab223192</a>) at 1/500 dilution

Predicted band size: 79 kDa

Observed band size: 60 kDa

false

Indirect ELISA - Anti-Tau (phospho T181) antibody [EPR22064] - BSA and Azide free (AB240579)
  • I-ELISA

Lab

Indirect ELISA - Anti-Tau (phospho T181) antibody [EPR22064] - BSA and Azide free (AB240579)

This data was developed using ab223192, the same antibody clone in a different buffer formulation.

Indirect ELISA analysis of ab223192 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1 : 2500 dilution.

Antigen : Human MAPT(phospho T181) peptide, Human MAPT non-phospho peptide

Antigen concentration : 1000 ng/ml.

Western blot - Anti-Tau (phospho T181) antibody [EPR22064] - BSA and Azide free (AB240579)
  • WB

Unknown

Western blot - Anti-Tau (phospho T181) antibody [EPR22064] - BSA and Azide free (AB240579)

Blocking and dilution buffer : 5% NFDM/TBST.

WB membrane in Lane 2 was treated with alkaline phosphatase for 1h at 37°C.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223192).

All lanes:

Western blot - Anti-Tau (phospho T181) antibody [EPR22064] (<a href='/en-us/products/primary-antibodies/tau-phospho-t181-antibody-epr22064-ab223192'>ab223192</a>) at 1/1000 dilution

Lane 1:

Human brain lysate at 10 µg

Lane 2:

Human brain lysate (WB membrane treated with alkaline phosphatase) at 10 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 79 kDa

Observed band size: 60 kDa

false

Exposure time: 3min

Dot Blot - Anti-Tau (phospho T181) antibody [EPR22064] - BSA and Azide free (AB240579)
  • Dot

Unknown

Dot Blot - Anti-Tau (phospho T181) antibody [EPR22064] - BSA and Azide free (AB240579)

Dot blot analysis of Tau (phospho T181) labeled with ab223192 at 1/1000 dilution.

Lane 1 : Tau (phospho T181) peptide.
Lane 2 : Tau non-phospho peptide.

Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used for detection at 1/100000 dilution.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 10 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223192).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22064

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, Dot, IP, I-ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The specificity of this antibody refers to P10636-8.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Synthetic peptide": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Synthetic peptide - Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p></p>" } } }
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Product details

ab240579 is the carrier-free version of ab223192.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.
Biological function summary

Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.

Pathways

Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.

Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed : 21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed : 21985311, PubMed : 32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
See full target information MAPT phospho T181
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 31: PubMed39450567

2024

S100A16 stabilizes the ITGA3‑mediated ECM‑receptor interaction pathway to drive the malignant properties of lung adenocarcinoma cells via binding MOV10.

Applications

Unspecified application

Species

Unspecified reactive species

Lianren Yang,Ajuan Shen,Rujun Wang,Zhihui Zheng
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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