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Rabbit Recombinant Monoclonal TAU phospho T181 antibody. Suitable for IP, I-ELISA, Dot, WB, IHC-Fr, IHC-P, AP and reacts with Mouse, Rat, Human, Synthetic peptide - Mouse samples. Cited in 3 publications.


Images

Immunoprecipitation - Anti-Tau (phospho T181) antibody [EPR23506-107] (AB254409), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T181) antibody [EPR23506-107] (AB254409), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Tau (phospho T181) antibody [EPR23506-107] (AB254409), expandable thumbnail
  • Immunoprecipitation - Anti-Tau (phospho T181) antibody [EPR23506-107] (AB254409), expandable thumbnail
  • Western blot - Anti-Tau (phospho T181) antibody [EPR23506-107] (AB254409), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPI-ELISADotWBIHC-FrIHC-PAP
Human
Not recommended
Expected
Expected
Expected
Not recommended
Expected
Not recommended
Tested
Mouse
Not recommended
Tested
Expected
Expected
Tested
Tested
Tested
Expected
Rat
Not recommended
Tested
Expected
Expected
Tested
Tested
Tested
Expected
Synthetic peptide - Mouse
Not recommended
Not recommended
Tested
Tested
Not recommended
Not recommended
Not recommended
Not recommended

Not recommended
Not recommended

Species

Mouse

Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species

Human

Dilution info
-
Notes

-

Species

Rat

Dilution info
-
Notes

-

Species

Synthetic peptide - Mouse

Dilution info
-
Notes

-

Tested
Tested

Species

Mouse

Dilution info
1/30
Notes

-

Species

Rat

Dilution info
1/30
Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide - Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Synthetic peptide - Mouse

Dilution info
-
Notes

Use at 62.5 ng /mL.

Expected
Expected

Species

Mouse, Rat, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Synthetic peptide - Mouse

Dilution info
1/1000
Notes

-

Expected
Expected

Species

Mouse, Rat, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info
1/1000
Notes

-

Species

Rat

Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species

Human, Synthetic peptide - Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info
1/50
Notes

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Species

Rat

Dilution info
1/50
Notes

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide - Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info
1/5000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species

Rat

Dilution info
1/5000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Human

Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species

Synthetic peptide - Mouse

Dilution info
-
Notes

-

Tested
Tested

Species

Human

Dilution info
-
Notes

Antibody concentration range - 6.67, 3.33, 1.67, 0.83, 0.42, 0 nM/mL

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide - Mouse

Dilution info

-

Notes

-

Target data

Function

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed:21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed:21985311, PubMed:32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

Targets

MAPT phospho T181

Alternative names

Recommended products

Rabbit Recombinant Monoclonal TAU phospho T181 antibody. Suitable for IP, I-ELISA, Dot, WB, IHC-Fr, IHC-P, AP and reacts with Mouse, Rat, Human, Synthetic peptide - Mouse samples. Cited in 3 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR23506-107

Purification technique

Affinity purification Protein A

Specificity

The specificity of this antibody refers to P10636-8.

Dissociation constant
6.41 x 10-11 M
Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.

Biological function summary

Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.

Pathways

Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.

Associated diseases and disorders

Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

11 product images

  • Immunoprecipitation - Anti-Tau (phospho T181) antibody [EPR23506-107] (ab254409), expandable thumbnail

    Immunoprecipitation - Anti-Tau (phospho T181) antibody [EPR23506-107] (ab254409)

    Tau (phospho T181) was immunoprecipitated from 0.35 mg of mouse brain tissue lysate with ab254409 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab254409 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366), was used as secondary antibody at 1/5000 dilution.

    Lane 1: Mouse brain tissue lysate 10μg (Input).
    Lane 2: ab254409 IP in mouse brain tissue lysate.
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab254409 in mouse brain tissue lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 5.5 seconds.

    All lanes: Immunoprecipitation - Anti-Tau (phospho T181) antibody [EPR23506-107] (ab254409)

    Predicted band size: 47 kDa, 78 kDa

    Observed band size: 50-70 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T181) antibody [EPR23506-107] (ab254409), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T181) antibody [EPR23506-107] (ab254409)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Tau (phospho T181) with ab254409 at 1/5000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on mouse cerebrum without alkaline phosphatase treatment (image A). No signal was detected when tissues were treated with alkaline phosphatase (image B). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Frozen sections) - Anti-Tau (phospho T181) antibody [EPR23506-107] (ab254409), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Tau (phospho T181) antibody [EPR23506-107] (ab254409)

    Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized mouse cerebrum tissue labeling Tau (phospho T181) with ab254409 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse cerebrum without alkaline phosphatase treatment (image A). No signal was detected when tissues were treated with alkaline phosphatase (image B). The nuclear counterstain is DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Immunoprecipitation - Anti-Tau (phospho T181) antibody [EPR23506-107] (ab254409), expandable thumbnail

    Immunoprecipitation - Anti-Tau (phospho T181) antibody [EPR23506-107] (ab254409)

    Tau (phospho T181) was immunoprecipitated from 0.35 mg of rat brain tissue lysate with ab254409 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab254409 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366), was used as secondary antibody at 1/5000 dilution.

    Lane 1: Rat brain tissue lysate 10μg (Input).
    Lane 2: ab254409 IP in rat brain tissue lysate.
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab254409 in rat brain tissue lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 10 seconds.

    All lanes: Immunoprecipitation - Anti-Tau (phospho T181) antibody [EPR23506-107] (ab254409)

    Predicted band size: 47 kDa, 78 kDa

    Observed band size: 50-70 kDa

  • Western blot - Anti-Tau (phospho T181) antibody [EPR23506-107] (ab254409), expandable thumbnail

    Western blot - Anti-Tau (phospho T181) antibody [EPR23506-107] (ab254409)

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 21437732).

    This blot was developed using a higher sensitivity ECL substrate.

    All lanes: Western blot - Anti-Tau (phospho T181) antibody [EPR23506-107] (ab254409) at 1/1000 dilution

    Lane 1: Mouse brain tissue lysate at 20 µg

    Lane 2: Mouse brain tissue lysate (phosphatase treated membrane) at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 47 kDa, 78 kDa

    Observed band size: 50-70 kDa

  • Western blot - Anti-Tau (phospho T181) antibody [EPR23506-107] (ab254409), expandable thumbnail

    Western blot - Anti-Tau (phospho T181) antibody [EPR23506-107] (ab254409)

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 21437732).

    This blot was developed using a higher sensitivity ECL substrate.

    All lanes: Western blot - Anti-Tau (phospho T181) antibody [EPR23506-107] (ab254409) at 1/1000 dilution

    Lane 1: Rat brain tissue lysate at 20 µg

    Lane 2: Rat brain tissue lysate (phosphatase treated membrane) at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 47 kDa, 78 kDa

    Observed band size: 50-70 kDa

  • Immunohistochemistry (Frozen sections) - Anti-Tau (phospho T181) antibody [EPR23506-107] (ab254409), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Tau (phospho T181) antibody [EPR23506-107] (ab254409)

    Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized rat cerebrum tissue labeling Tau (phospho T181) with ab254409 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on rat cerebrum without alkaline phosphatase treatment (image A). Nearly no signal was detected when tissues were treated with alkaline phosphatase (image B). The nuclear counterstain is DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T181) antibody [EPR23506-107] (ab254409), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T181) antibody [EPR23506-107] (ab254409)

    Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Tau (phospho T181) with ab254409 at 1/5000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on rat cerebrum without alkaline phosphatase treatment (image A, PMID: 30279741). No signal was detected when tissues were treated with alkaline phosphatase (image B). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Indirect ELISA - Anti-Tau (phospho T181) antibody [EPR23506-107] (ab254409), expandable thumbnail

    Indirect ELISA - Anti-Tau (phospho T181) antibody [EPR23506-107] (ab254409)

    Antigen: Mouse Tau non-phospho peptide,Mouse Tau (phospho T181) peptide.

    Antigen concentration: 1000 ng/mL.

    ab254409 used at 0 - 2000 ng/mL.

    Secondary antibody: Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution.

  • Dot Blot - Anti-Tau (phospho T181) antibody [EPR23506-107] (ab254409), expandable thumbnail

    Dot Blot - Anti-Tau (phospho T181) antibody [EPR23506-107] (ab254409)

    Dot blot analysis of Tau (phospho T181) labeled with ab254409 at 1/1000 dilution.

    Lane 1: Tau (phospho T181) peptide (aa 177-190).
    Lane 2: Tau non-phospho peptide (aa 174-190).

    Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution was used as secondary antibody.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Affinity Purification - Anti-Tau (phospho T181) antibody [EPR23506-107] (ab254409), expandable thumbnail

    Affinity Purification - Anti-Tau (phospho T181) antibody [EPR23506-107] (ab254409)

    Biotinylated Human Tau phospho T181 [0.5 μg/ml] was loaded to SA biosensor on Fortebio RED96e Machine, then associate with recombinant Anti-Tau (phospho T181) antibody [EPR23506-107] in serial concentration points [6.67, 3.33, 1.67, 0.83, 0.42 nM/mL] by 2-fold dilution, next to dissociate in blank testing buffer [0.1% BSA in PBST (0.05%Tween-20)]. Calculated signals had already subtracted blank control, curve fitting using 1:1 binding model. Non-phospho Tau protein's association and dissociation were also showed in graph. KD (M) value of Anti-Tau (phospho T181) antibody [EPR23506-107] is 6.41E-11

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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