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AB181206

Anti-Tau (phospho T205) antibody [EPR2403(2)]

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(5 Publications)

Rabbit Recombinant Monoclonal TAU phospho T205 antibody. Suitable for Dot, WB and reacts with Synthetic peptide, Human samples. Cited in 5 publications.

View Alternative Names

MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

3 Images
Western blot - Anti-Tau (phospho T205) antibody [EPR2403(2)] (AB181206)
  • WB

Lab

Western blot - Anti-Tau (phospho T205) antibody [EPR2403(2)] (AB181206)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Tau (phospho T205) antibody [EPR2403(2)] (ab181206) at 1/1000 dilution

Lane 1:

SK-N-BE(2) (human neuroblastoma neuroblast) whole cell lysate at 10 µg

Lane 2:

SK-N-BE(2) (human neuroblastoma neuroblast) whole cell lysate. The membrane was incubated with phosphatase at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 78 kDa

Observed band size: 55 kDa

false

Exposure time: 3min

Western blot - Anti-Tau (phospho T205) antibody [EPR2403(2)] (AB181206)
  • WB

Supplier Data

Western blot - Anti-Tau (phospho T205) antibody [EPR2403(2)] (AB181206)

All lanes:

Western blot - Anti-Tau (phospho T205) antibody [EPR2403(2)] (ab181206) at 1/50000 dilution

Lane 1:

Human fetal brain lysate at 10 µg

Lane 2:

Human fetal brain treated with alkaline phosphatase at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 78 kDa

false

Dot Blot - Anti-Tau (phospho T205) antibody [EPR2403(2)] (AB181206)
  • Dot

Lab

Dot Blot - Anti-Tau (phospho T205) antibody [EPR2403(2)] (AB181206)

Dot blot analysis of Tau (pS205) peptide (Lane 1) and Tau non-phospho peptide (Lane 2) labelling Tau (pS205) with ab181206 at a dilution of 1/1000. ab97051 (peroxidase-conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/100000.

Exposure time : 3 minutes.
Blocking and dilution buffer : 5% NFDM/TBST.

  • Carrier free

    Anti-Tau (phospho T205) antibody [EPR2403(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2403(2)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The specificity of this antibody refers to P10636-8.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Synthetic peptide": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.
Biological function summary

Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.

Pathways

Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.

Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The protein expressed by the MAPT gene promotes microtubule assembly and stability and might be involved in establishing and maintaining neuronal polarity. Its C-terminus binds axonal microtubules, and the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between the two. Axonal polarity is predetermined by MAPT localization within the neuronal cell's domain defined by the centrosome. The short isoforms allow cytoskeleton plasticity, whereas the longer isoforms may preferentially play a role in its stabilization. This supplementary information is collated from multiple sources and compiled automatically.
See full target information MAPT phospho T205
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Brain, behavior, & immunity - health 28:100568 PubMed36704658

2023

Systemic Inflammation Causes Microglial Dysfunction With a Vascular AD phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Praveen Bathini,Isabel Dupanloup,Elena Zenaro,Eleonora Terrabuio,Amrei Fischer,Edona Ballabani,Marie-Agnes Doucey,Lavinia Alberi

Science advances 8:eadd2577 PubMed36459557

2022

Treatment of epilepsy using a targeted p38γ kinase gene therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Nicolle Morey,Magdalena Przybyla,Julia van der Hoven,Yazi D Ke,Fabien Delerue,Janet van Eersel,Lars M Ittner

Neuro-degenerative diseases 21:117-125 PubMed35272285

2022

Neuroprotective and Therapeutic Effects of Tocovid and Twendee-X on Aβ Oligomer-Induced Damage in the SH-SY5Y Cell Line.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Hu,Toru Yamashita,Haibo Yu,Zhihong Bian,Xinran Hu,Tian Feng,Koh Tadokoro,Ryuta Morihara,Koji Abe

Acta neuropathologica communications 7:192 PubMed31796124

2019

A post-translational modification signature defines changes in soluble tau correlating with oligomerization in early stage Alzheimer's disease brain.

Applications

Unspecified application

Species

Unspecified reactive species

Ebru Ercan-Herbst,Jens Ehrig,David C Schöndorf,Annika Behrendt,Bernd Klaus,Borja Gomez Ramos,Nuria Prat Oriol,Christian Weber,Dagmar E Ehrnhoefer

Molecular neurodegeneration 12:87 PubMed29157277

2017

A validated antibody panel for the characterization of tau post-translational modifications.

Applications

ICC/IF

Species

Unspecified reactive species

Ebru Ercan,Sameh Eid,Christian Weber,Alexandra Kowalski,Maria Bichmann,Annika Behrendt,Frank Matthes,Sybille Krauss,Peter Reinhardt,Simone Fulle,Dagmar E Ehrnhoefer
View all publications
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Product promise

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