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Rabbit Recombinant Monoclonal TAU phospho S214 + T212 antibody. Suitable for IHC-P, I-ELISA, Dot and reacts with Transfected cell line - Human, Human, Synthetic peptide samples.

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Images

Indirect ELISA - Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] (AB324015), expandable thumbnail
  • Dot Blot - Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] (AB324015), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] (AB324015), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] (AB324015), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] (AB324015), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PI-ELISADotWB
Human
Tested
Expected
Expected
Not recommended
Synthetic peptide
Not recommended
Tested
Tested
Not recommended
Transfected cell line - Human
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Transfected cell line - Human
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Synthetic peptide
Dilution info
62.5 ng/mL
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Transfected cell line - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Synthetic peptide
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Transfected cell line - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Transfected cell line - Human, Synthetic peptide
Dilution info
-
Notes

-

Target data

Function

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed:21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed:21985311, PubMed:32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

Alternative names

MAPTL, MTBT1, TAU, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

Rabbit Recombinant Monoclonal TAU phospho S214 + T212 antibody. Suitable for IHC-P, I-ELISA, Dot and reacts with Transfected cell line - Human, Human, Synthetic peptide samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogens
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR27523-252
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Indirect ELISA - Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] (ab324015), expandable thumbnail

    Indirect ELISA - Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] (ab324015)

    Indirect ELISA analysis of ab324015 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution dilution.

    Antigen: Tau (phospho pT212, pS214) peptide, Tau (phospho pT212) peptide, Tau (phospho pS214) peptide, Tau non-phospho peptide.

    Antigen concentration: 1000 ng/ml

  • Dot Blot - Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] (ab324015), expandable thumbnail

    Dot Blot - Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] (ab324015)

    Tau (phospho T212 + S214) Dot Blot staining using rabbit Anti-Tau (phospho T212 + S214) antibody

    Dot blot analysis of Tau (phospho T212 + S214) using ab324015 at 1:1000 (0.515 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.

    Lane1: Tau (phospho pT212, pS214) peptide

    Lane2: Tau (phospho pT212) peptide

    Lane3: Tau (phospho pS214) peptide

    Lane4: Tau non-phospho peptide

    Exposure time: 180 seconds.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    All lanes: Dot Blot - Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] (ab324015) at 1/1000 dilution

    Lane 1: Tau (phospho pT212, pS214) peptide

    Lane 2: Tau (phospho pT212) peptide

    Lane 3: Tau (phospho pS214) peptide

    Lane 4: Tau non-phospho peptide

    Secondary

    All lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Exposure time: 180s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] (ab324015), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] (ab324015)

    Tau (phospho T212 + S214) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-Tau (phospho T212 + S214) antibody

    Immunohistochemical analysis of paraffin-embedded (A) HEK-293T(human embryonic kidney epithelial cell) transfected with a Tau 2N4R expression vector containing a Myc-his tag cell pellet treated with Okadaic Acid(1uM 60min) and Calyculin A(200nM 60min) without Alkaline Phosphatase treatment. (B) HEK-293T(human embryonic kidney epithelial cell) transfected with a Tau 2N4R expression vector containing a Myc-his tag cell pellet treated with Okadaic Acid(1uM 60min) and Calyculin A(200nM 60min) with Alkaline Phosphatase treatment. (C) HEK-293T transfected with a Tau 2N4R (mutant T212A) expression vector containing a Myc-his tag cell pellet treated with Okadaic Acid(1uM 60min) and Calyculin A(200nM 60min). (D) HEK-293T transfected with a Tau 2N4R (mutant T212A, S214A) expression vector containing a Myc-his tag cell pellet treated with Okadaic Acid(1uM 60min) and Calyculin A(200nM 60min). tissue labeling Tau (phospho T212 + S214) with ab324015 at 1/1000 (0.515 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Positive staining on (A) HEK-293T transfected with a Tau 2N4R expression vector containing a Myc-his tag cell pellet treated with Okadaic Acid(1uM 60min) and Calyculin A(200nM 60min) without Alkaline Phosphatase treatment. No staining on (B) HEK-293T transfected with a Tau 2N4R expression vector containing a Myc-his tag cell pellet treated with Okadaic Acid(1uM 60min) and Calyculin A(200nM 60min) with Alkaline Phosphatase treatment, (C) HEK-293T transfected with a Tau 2N4R (mutant T212A) expression vector containing a Myc-his tag cell pellet treated with Okadaic Acid(1uM 60min) and Calyculin A(200nM 60min) and (D) HEK-293T transfected with a Tau 2N4R (mutant T212A, S214A) expression vector containing a Myc-his tag cell pellet treated with Okadaic Acid(1uM 60min) and Calyculin A(200nM 60min).

    The primary antibody was incubated for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] (ab324015), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] (ab324015)

    Tau (phospho T212 + S214) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human colon using rabbit Anti-Tau (phospho T212 + S214) antibody

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Tau (phospho T212 + S214) with ab324015 at 1/1000 (0.515 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Negative control: No staining on human colon.

    The primary antibody was incubated for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] (ab324015), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] (ab324015)

    Tau (phospho T212 + S214) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human cerebrum using rabbit Anti-Tau (phospho T212 + S214) antibody

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Tau (phospho T212 + S214) with ab324015 at 1/1000 (0.515 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Low expression tissue: No staining on human cerebrum.

    The primary antibody was incubated for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] (ab324015), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] (ab324015)

    Tau (phospho T212 + S214) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-Tau (phospho T212 + S214) antibody

    Immunohistochemical analysis of paraffin-embedded Human Alzheimer's disease brain tissue labeling Tau (phospho T212 + S214) with ab324015 at 1/1000 (0.515 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Positive staining on (A) Human Alzheimer's disease brain without Alkaline Phosphatase treatment, no staining on (B) Human Alzheimer's disease brain with Alkaline Phosphatase treatment (PMID: 31722749).

    The primary antibody was incubated for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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