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AB324285

Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] - BSA and Azide free

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Rabbit Recombinant Monoclonal TAU phospho S214 + T212 antibody. Carrier free. Suitable for IHC-P, I-ELISA, Dot and reacts with Transfected cell line - Human, Human, Synthetic peptide samples.

View Alternative Names

MAPTL, MTBT1, TAU, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] - BSA and Azide free (AB324285)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] - BSA and Azide free (AB324285)

This data was developed using ab324015, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Tau (phospho T212 + S214) with ab324015 at 1/1000 (0.515 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Low expression tissue : No staining on human cerebrum.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] - BSA and Azide free (AB324285)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] - BSA and Azide free (AB324285)

This data was developed using ab324015, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Tau (phospho T212 + S214) with ab324015 at 1/1000 (0.515 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Negative control : No staining on human colon.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] - BSA and Azide free (AB324285)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] - BSA and Azide free (AB324285)

This data was developed using ab324015, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded (A) HEK-293T(human embryonic kidney epithelial cell) transfected with a Tau 2N4R expression vector containing a Myc-his tag cell pellet treated with Okadaic Acid(1uM 60min) and Calyculin A(200nM 60min) without Alkaline Phosphatase treatment. (B) HEK-293T(human embryonic kidney epithelial cell) transfected with a Tau 2N4R expression vector containing a Myc-his tag cell pellet treated with Okadaic Acid(1uM 60min) and Calyculin A(200nM 60min) with Alkaline Phosphatase treatment. (C) HEK-293T transfected with a Tau 2N4R (mutant T212A) expression vector containing a Myc-his tag cell pellet treated with Okadaic Acid(1uM 60min) and Calyculin A(200nM 60min). (D) HEK-293T transfected with a Tau 2N4R (mutant T212A, S214A) expression vector containing a Myc-his tag cell pellet treated with Okadaic Acid(1uM 60min) and Calyculin A(200nM 60min). tissue labeling Tau (phospho T212 + S214) with ab324015 at 1/1000 (0.515 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Positive staining on (A) HEK-293T transfected with a Tau 2N4R expression vector containing a Myc-his tag cell pellet treated with Okadaic Acid(1uM 60min) and Calyculin A(200nM 60min) without Alkaline Phosphatase treatment. No staining on (B) HEK-293T transfected with a Tau 2N4R expression vector containing a Myc-his tag cell pellet treated with Okadaic Acid(1uM 60min) and Calyculin A(200nM 60min) with Alkaline Phosphatase treatment, (C) HEK-293T transfected with a Tau 2N4R (mutant T212A) expression vector containing a Myc-his tag cell pellet treated with Okadaic Acid(1uM 60min) and Calyculin A(200nM 60min) and (D) HEK-293T transfected with a Tau 2N4R (mutant T212A, S214A) expression vector containing a Myc-his tag cell pellet treated with Okadaic Acid(1uM 60min) and Calyculin A(200nM 60min).
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] - BSA and Azide free (AB324285)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] - BSA and Azide free (AB324285)

This data was developed using ab324015, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human Alzheimer's disease brain tissue labeling Tau (phospho T212 + S214) with ab324015 at 1/1000 (0.515 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Positive staining on (A) Human Alzheimer's disease brain without Alkaline Phosphatase treatment, no staining on (B) Human Alzheimer's disease brain with Alkaline Phosphatase treatment (PMID : 31722749).
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Indirect ELISA - Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] - BSA and Azide free (AB324285)
  • I-ELISA

Supplier Data

Indirect ELISA - Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] - BSA and Azide free (AB324285)

This data was developed using ab324015, the same antibody clone in a different buffer formulation.

Indirect ELISA analysis of ab324015 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1 : 2500 dilution dilution.

Antigen : Tau (phospho pT212, pS214) peptide, Tau (phospho pT212) peptide, Tau (phospho pS214) peptide, Tau non-phospho peptide.

Antigen concentration : 1000 ng/ml

Dot Blot - Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] - BSA and Azide free (AB324285)
  • Dot

Supplier Data

Dot Blot - Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] - BSA and Azide free (AB324285)

This data was developed using ab324015, the same antibody clone in a different buffer formulation.

Dot blot analysis of Tau (phospho T212 + S214) using ab324015 at 1 : 1000 (0.515 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Lane1 : Tau (phospho pT212, pS214) peptide
Lane2 : Tau (phospho pT212) peptide
Lane3 : Tau (phospho pS214) peptide
Lane4 : Tau non-phospho peptide

Exposure time : 180 seconds.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Dot Blot - Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] (<a href='/en-us/products/primary-antibodies/tau-phospho-t212-s214-antibody-epr27523-252-ab324015'>ab324015</a>) at 1/1000 dilution

Lane 1:

Tau (phospho pT212, pS214) peptide

Lane 2:

Tau (phospho pT212) peptide

Lane 3:

Tau (phospho pS214) peptide

Lane 4:

Tau non-phospho peptide

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s

  • Unconjugated

    Anti-Tau (phospho T212 + S214) antibody [EPR27523-252]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR27523-252

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Dot, I-ELISA, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Synthetic peptide": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p></p>", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell line - Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

ab324285 is the carrier-free version of ab324015

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed : 21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed : 21985311, PubMed : 32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
See full target information MAPT phospho S214 + T212
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com