Rabbit Recombinant Monoclonal TAU phospho S214 + T212 antibody. Carrier free. Suitable for IHC-P, I-ELISA, Dot and reacts with Transfected cell line - Human, Human, Synthetic peptide samples.
Images
![Dot Blot - Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] - BSA and Azide free (AB324285), expandable thumbnail](https://content.abcam.com/products/images/tau-phospho-t212-s214-antibody-epr27523-252-bsa-and-azide-free-ab324285--dot-blot-img445894.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] - BSA and Azide free (AB324285)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] - BSA and Azide free (AB324285), expandable thumbnail](https://content.abcam.com/products/images/tau-phospho-t212-s214-antibody-epr27523-252-bsa-and-azide-free-ab324285--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img445865.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] - BSA and Azide free (AB324285)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] - BSA and Azide free (AB324285), expandable thumbnail](https://content.abcam.com/products/images/tau-phospho-t212-s214-antibody-epr27523-252-bsa-and-azide-free-ab324285--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img445866.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] - BSA and Azide free (AB324285)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] - BSA and Azide free (AB324285), expandable thumbnail](https://content.abcam.com/products/images/tau-phospho-t212-s214-antibody-epr27523-252-bsa-and-azide-free-ab324285--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img445867.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] - BSA and Azide free (AB324285)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] - BSA and Azide free (AB324285), expandable thumbnail](https://content.abcam.com/products/images/tau-phospho-t212-s214-antibody-epr27523-252-bsa-and-azide-free-ab324285--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img445868.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] - BSA and Azide free (AB324285)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | I-ELISA | Dot | WB | |
|---|---|---|---|---|
| Human | Tested | Expected | Expected | Not recommended |
| Synthetic peptide | Not recommended | Tested | Tested | Not recommended |
| Transfected cell line - Human | Tested | Not recommended | Not recommended | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
I-ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Human | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Human | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Transfected cell line - Human, Synthetic peptide | Dilution info - | Notes - |
Target data
Function
The protein expressed by the MAPT gene promotes microtubule assembly and stability and might be involved in establishing and maintaining neuronal polarity. Its C-terminus binds axonal microtubules, and the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between the two. Axonal polarity is predetermined by MAPT localization within the neuronal cell's domain defined by the centrosome. The short isoforms allow cytoskeleton plasticity, whereas the longer isoforms may preferentially play a role in its stabilization. This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
MAPTL, MTBT1, TAU, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau
Rabbit Recombinant Monoclonal TAU phospho S214 + T212 antibody. Carrier free. Suitable for IHC-P, I-ELISA, Dot and reacts with Transfected cell line - Human, Human, Synthetic peptide samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogens
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR27523-252
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab324285 is the carrier-free version of Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] ab324015
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Dot Blot - Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] - BSA and Azide free (ab324285), expandable thumbnail](https://content.abcam.com/products/images/tau-phospho-t212-s214-antibody-epr27523-252-bsa-and-azide-free-ab324285--dot-blot-img445894.jpg "Dot Blot - Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] - BSA and Azide free (ab324285)")
Dot Blot - Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] - BSA and Azide free (ab324285)
Tau (phospho T212 + S214) Dot Blot staining using rabbit Anti-Tau (phospho T212 + S214) antibody
This data was developed using Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] ab324015, the same antibody clone in a different buffer formulation.
Dot blot analysis of Tau (phospho T212 + S214) using Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] ab324015 at 1:1000 (0.515 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.
Lane1: Tau (phospho pT212, pS214) peptide
Lane2: Tau (phospho pT212) peptide
Lane3: Tau (phospho pS214) peptide
Lane4: Tau non-phospho peptideExposure time: 180 seconds.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
All lanes: Dot Blot - Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] (Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] ab324015) at 1/1000 dilution
Lane 1: Tau (phospho pT212, pS214) peptide
Lane 2: Tau (phospho pT212) peptide
Lane 3: Tau (phospho pS214) peptide
Lane 4: Tau non-phospho peptide
SecondaryAll lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Exposure time: 180s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] - BSA and Azide free (ab324285), expandable thumbnail](https://content.abcam.com/products/images/tau-phospho-t212-s214-antibody-epr27523-252-bsa-and-azide-free-ab324285--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img445865.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] - BSA and Azide free (ab324285)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] - BSA and Azide free (ab324285)
Tau (phospho T212 + S214) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-Tau (phospho T212 + S214) antibody
This data was developed using Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] ab324015, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human Alzheimer's disease brain tissue labeling Tau (phospho T212 + S214) with Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] ab324015 at 1/1000 (0.515 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Positive staining on (A) Human Alzheimer's disease brain without Alkaline Phosphatase treatment, no staining on (B) Human Alzheimer's disease brain with Alkaline Phosphatase treatment (PMID: 31722749).
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] - BSA and Azide free (ab324285), expandable thumbnail](https://content.abcam.com/products/images/tau-phospho-t212-s214-antibody-epr27523-252-bsa-and-azide-free-ab324285--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img445866.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] - BSA and Azide free (ab324285)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] - BSA and Azide free (ab324285)
Tau (phospho T212 + S214) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human cerebrum using rabbit Anti-Tau (phospho T212 + S214) antibody
This data was developed using Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] ab324015, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Tau (phospho T212 + S214) with Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] ab324015 at 1/1000 (0.515 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Low expression tissue: No staining on human cerebrum.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] - BSA and Azide free (ab324285), expandable thumbnail](https://content.abcam.com/products/images/tau-phospho-t212-s214-antibody-epr27523-252-bsa-and-azide-free-ab324285--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img445867.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] - BSA and Azide free (ab324285)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] - BSA and Azide free (ab324285)
Tau (phospho T212 + S214) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human colon using rabbit Anti-Tau (phospho T212 + S214) antibody
This data was developed using Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] ab324015, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Tau (phospho T212 + S214) with Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] ab324015 at 1/1000 (0.515 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Negative control: No staining on human colon.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] - BSA and Azide free (ab324285), expandable thumbnail](https://content.abcam.com/products/images/tau-phospho-t212-s214-antibody-epr27523-252-bsa-and-azide-free-ab324285--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img445868.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] - BSA and Azide free (ab324285)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] - BSA and Azide free (ab324285)
Tau (phospho T212 + S214) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-Tau (phospho T212 + S214) antibody
This data was developed using Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] ab324015, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded (A) HEK-293T(human embryonic kidney epithelial cell) transfected with a Tau 2N4R expression vector containing a Myc-his tag cell pellet treated with Okadaic Acid(1uM 60min) and Calyculin A(200nM 60min) without Alkaline Phosphatase treatment. (B) HEK-293T(human embryonic kidney epithelial cell) transfected with a Tau 2N4R expression vector containing a Myc-his tag cell pellet treated with Okadaic Acid(1uM 60min) and Calyculin A(200nM 60min) with Alkaline Phosphatase treatment. (C) HEK-293T transfected with a Tau 2N4R (mutant T212A) expression vector containing a Myc-his tag cell pellet treated with Okadaic Acid(1uM 60min) and Calyculin A(200nM 60min). (D) HEK-293T transfected with a Tau 2N4R (mutant T212A, S214A) expression vector containing a Myc-his tag cell pellet treated with Okadaic Acid(1uM 60min) and Calyculin A(200nM 60min). tissue labeling Tau (phospho T212 + S214) with Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] ab324015 at 1/1000 (0.515 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Positive staining on (A) HEK-293T transfected with a Tau 2N4R expression vector containing a Myc-his tag cell pellet treated with Okadaic Acid(1uM 60min) and Calyculin A(200nM 60min) without Alkaline Phosphatase treatment. No staining on (B) HEK-293T transfected with a Tau 2N4R expression vector containing a Myc-his tag cell pellet treated with Okadaic Acid(1uM 60min) and Calyculin A(200nM 60min) with Alkaline Phosphatase treatment, (C) HEK-293T transfected with a Tau 2N4R (mutant T212A) expression vector containing a Myc-his tag cell pellet treated with Okadaic Acid(1uM 60min) and Calyculin A(200nM 60min) and (D) HEK-293T transfected with a Tau 2N4R (mutant T212A, S214A) expression vector containing a Myc-his tag cell pellet treated with Okadaic Acid(1uM 60min) and Calyculin A(200nM 60min).
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Indirect ELISA - Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] - BSA and Azide free (ab324285), expandable thumbnail](https://content.abcam.com/products/images/tau-phospho-t212-s214-antibody-epr27523-252-bsa-and-azide-free-ab324285--indirect-elisa-img445898.jpg "Indirect ELISA - Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] - BSA and Azide free (ab324285)")
Indirect ELISA - Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] - BSA and Azide free (ab324285)
This data was developed using Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] ab324015, the same antibody clone in a different buffer formulation.
Indirect ELISA analysis of Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] ab324015 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution dilution.
Antigen: Tau (phospho pT212, pS214) peptide, Tau (phospho pT212) peptide, Tau (phospho pS214) peptide, Tau non-phospho peptide.
Antigen concentration: 1000 ng/ml
![Indirect ELISA - Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] - BSA and Azide free (ab324285), expandable thumbnail](https://content.abcam.com/products/images/tau-phospho-t212-s214-antibody-epr27523-252-bsa-and-azide-free-ab324285--indirect-elisa-img445898.jpg/_jcr_content/renditions/webp-475.webp "Indirect ELISA - Anti-Tau (phospho T212 + S214) antibody [EPR27523-252] - BSA and Azide free (ab324285)")
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