AB291080

Anti-Tau (phospho T217) antibody [EPR24654-110]

20ul selling size
RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(1 Publication)

Anti-Tau (phospho T217) antibody [EPR24654-110] (ab291080) is a rabbit monoclonal antibody detecting Tau in Western Blot, IP, ICC/IF, ELISA, Dot Blot, and IHC-p. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau, MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T217) antibody [EPR24654-110] (AB291080)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T217) antibody [EPR24654-110] (AB291080)

Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling Tau (phospho T217) with ab291080 at 1/25000 dilution (0.02 μg/ml).

Negative control : no staining on human stomach.

The section was incubated with ab291080 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument using LeicaDS9800 (Bond™ Polymer Refine Detection) secondary antibody, ready to use.

Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T217) antibody [EPR24654-110] (AB291080)

Immunohistochemical analysis of paraffin-embedded Human normal brain tissue labeling Tau (phospho T217) with ab291080 at 1/25000 dilution (0.02 μg/ml).

Negative control : no staining on human normal brain.

The section was incubated with ab291080 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument using LeicaDS9800 (Bond™ Polymer Refine Detection) secondary antibody, ready to use.

Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T217) antibody [EPR24654-110] (AB291080)

Immunohistochemical analysis of paraffin-embedded Human Alzheimer's disease brain tissue labeling Tau (phospho T217) with ab291080 at 1/25000 dilution (0.02 μg/ml).

Positive staining on human Alzheimer's disease brain.

The section was incubated with primary antibody ab291080 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

The secondary antibody used was LeicaDS9800 (Bond™ Polymer Refine Detection), ready to use.

Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed with Sodium-Citrate buffer (pH 6.0, Epitope Retrieval Solution1) for 20 mins.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T217) antibody [EPR24654-110] (AB291080)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling Tau (phospho T217) with ab291080 at 1/25000 dilution (0.02 μg/ml).

Negative control : no staining on human skeletal muscle.

The section was incubated with ab291080 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument using LeicaDS9800 (Bond™ Polymer Refine Detection) secondary antibody, ready to use.

Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

Indirect ELISA - Anti-Tau (phospho T217) antibody [EPR24654-110] (AB291080)

I-ELISA

Lab

Indirect ELISA - Anti-Tau (phospho T217) antibody [EPR24654-110] (AB291080)

Antigen human Tau (phospho T217) peptide and a control human Tau non-phospho peptide were at 1000 ng/ml. ab291080 was used at 1000-0 ng/ml. Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1/2500 dilution.

Lab

Western blot - Anti-Tau (phospho T217) antibody [EPR24654-110] (AB291080)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200,000 dilution.

In Western blot, Anti-Tau antibody [EP2456Y] (ab76128) (1/1000) (55KDa)

This blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid femtogram range.

All lanes:

Western blot - Anti-Tau (phospho T217) antibody [EPR24654-110] (ab291080) at 1/1000 dilution

Lane 1:

Rat P0 brain (Untreated membrane) at 20 µg

Lane 2:

Rat P0 brain (Phosphatase treated membrane) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 50-80 kDa,36 kDa,55 kDa

true

Exposure time: 180s

Lab

Dot Blot - Anti-Tau (phospho T217) antibody [EPR24654-110] (AB291080)

Blocking/diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Dot Blot - Anti-Tau (phospho T217) antibody [EPR24654-110] (ab291080) at 1/1000 dilution

Lane 1:

Tau (phospho T217) peptide

Lane 2:

Tau (phospho T220) peptide

Lane 3:

Tau non-phospho T217 peptide

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 3min

Carrier free

Anti-Tau (phospho T217) antibody [EPR24654-110] (BSA and Azide free)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24654-110

Isotype

IgG

Carrier free

Reacts with

Human, Rat

Applications

WB, Dot, I-ELISA, ICC/IF, IHC-P, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The specificity of this antibody refers to P10636-8.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/2000", "ICCIF-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/25000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Mouse": { "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Synthetic peptide - Human": { "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

Product details

What is this antibody validated in?
Anti-Tau (phospho T217) antibody [EPR24654-110] (ab291080) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA, Dot Blot in Human samples.

What is the molecular weight of Tau?
Anti-Tau (phospho T217) [EPR24654-110] (ab291080) specifically detects a band for Tau (UniProt: P10636) at a molecular weight of 46kDa.

Other related products
We have a range of other formats of antibody clone [EPR24654-110] also available for your convenience: ab291080, Carrier free - ab291104

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 20µl. Discover our selection of trial-size antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.

Biological function summary

Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.

Pathways

Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3Β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.

Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed : 21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed : 21985311, PubMed : 32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

See full target information MAPT phospho T217

Additional targets

MAPT phospho T217

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Alzheimer's research & therapy 16:267 PubMed39702316

2024

Structural and functional alterations of neurons derived from sporadic Alzheimer's disease hiPSCs are associated with downregulation of the LIMK1-cofilin axis.

Applications

Unspecified application

Species

Unspecified reactive species

Raimondo Sollazzo,Domenica Donatella Li Puma,Giuseppe Aceto,Fabiola Paciello,Claudia Colussi,Maria Gabriella Vita,Guido Maria Giuffrè,Francesco Pastore,Alessia Casamassa,Jessica Rosati,Agnese Novelli,Sabrina Maietta,Francesco Danilo Tiziano,Camillo Marra,Cristian Ripoli,Claudio Grassi

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Tau (phospho T217) antibody [EPR24654-110]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T217) antibody [EPR24654-110] (AB291080)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T217) antibody [EPR24654-110] (AB291080)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T217) antibody [EPR24654-110] (AB291080)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T217) antibody [EPR24654-110] (AB291080)

Indirect ELISA - Anti-Tau (phospho T217) antibody [EPR24654-110] (AB291080)

Western blot - Anti-Tau (phospho T217) antibody [EPR24654-110] (AB291080)

Dot Blot - Anti-Tau (phospho T217) antibody [EPR24654-110] (AB291080)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (1)

Product promise