Anti-Tau (phospho T217) antibody [EPR24654-110] (ab291080) is a rabbit monoclonal antibody detecting Tau in Western Blot, IP, ICC/IF, ELISA, Dot Blot. Suitable for Human, Mouse.
- Biophysical QC for unrivalled batch-batch consistency
Images
![Western blot - Anti-Tau (phospho T217) antibody [EPR24654-110] (AB291080), expandable thumbnail](https://content.abcam.com/products/images/tau-phospho-t217-antibody-epr24654-110-ab291080--western-blot-img401963.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Tau (phospho T217) antibody [EPR24654-110] (AB291080)")
![Western blot - Anti-Tau (phospho T217) antibody [EPR24654-110] (AB291080), expandable thumbnail](https://content.abcam.com/products/images/tau-phospho-t217-antibody-epr24654-110-ab291080--western-blot-img401962.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Tau (phospho T217) antibody [EPR24654-110] (AB291080)")
![Immunocytochemistry/ Immunofluorescence - Anti-Tau (phospho T217) antibody [EPR24654-110] (AB291080), expandable thumbnail](https://content.abcam.com/products/images/tau-phospho-t217-antibody-epr24654-110-ab291080--immunocytochemistry-immunofluorescence-img401098.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Tau (phospho T217) antibody [EPR24654-110] (AB291080)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| I-ELISA | Dot | IP | ICC/IF | WB | IHC-P | |
|---|---|---|---|---|---|---|
| Human | Predicted | Predicted | Predicted | Predicted | Predicted | Not recommended |
| Mouse | Expected | Expected | Tested | Tested | Tested | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Synthetic peptide | Not recommended | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
| Synthetic peptide - Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
I-ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide, Rat | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human, Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/30 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human, Synthetic peptide, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/2000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human, Synthetic peptide, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human, Synthetic peptide, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Synthetic peptide - Human, Synthetic peptide | Dilution info - | Notes - |
Target data
Function
The protein expressed by the MAPT gene promotes microtubule assembly and stability and might be involved in establishing and maintaining neuronal polarity. Its C-terminus binds axonal microtubules, and the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between the two. Axonal polarity is predetermined by MAPT localization within the neuronal cell's domain defined by the centrosome. The short isoforms allow cytoskeleton plasticity, whereas the longer isoforms may preferentially play a role in its stabilization. This supplementary information is collated from multiple sources and compiled automatically.
Additional Targets
MAPT phospho T217
Alternative names
MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau
Recommended products
Anti-Tau (phospho T217) antibody [EPR24654-110] (ab291080) is a rabbit monoclonal antibody detecting Tau in Western Blot, IP, ICC/IF, ELISA, Dot Blot. Suitable for Human, Mouse.
- Biophysical QC for unrivalled batch-batch consistency
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR24654-110
- Purification technique
- Affinity purification Protein A
- Specificity
The specificity of this antibody refers to P10636-8.
- Dissociation constant
- 5.59 x 10-11 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-Tau (phospho T217) antibody [EPR24654-110] (ab291080) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA, Dot Blot in Human, Mouse samples.
What is the molecular weight of Tau?
Anti-Tau (phospho T217) [EPR24654-110] (ab291080) specifically detects a band for Tau (UniProt: P10636) at a molecular weight of 46kDa.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.
Other related products
We have a range of other formats of antibody clone [EPR24654-110] also available for your convenience:
ab291080, Carrier free - Anti-Tau (phospho T217) antibody [EPR24654-110] (BSA and Azide free) ab291104
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.
- Biological function summary
Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.
- Pathways
Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3Β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.
- Associated diseases and disorders
Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-Tau (phospho T217) antibody [EPR24654-110] (ab291080), expandable thumbnail](https://content.abcam.com/products/images/tau-phospho-t217-antibody-epr24654-110-ab291080--western-blot-img401963.jpg "Western blot - Anti-Tau (phospho T217) antibody [EPR24654-110] (ab291080)")
Western blot - Anti-Tau (phospho T217) antibody [EPR24654-110] (ab291080)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
All lanes: Western blot - Anti-Tau (phospho T217) antibody [EPR24654-110] (ab291080) at 1/1000 dilution
Lane 1: Mouse P5 brain tissue lysate (Untreated membrane) at 20 µg
Lane 2: Mouse P5 brain tissue lysate (Phosphatase treated membrane) at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Developed using the ECL technique.
![Western blot - Anti-Tau (phospho T217) antibody [EPR24654-110] (ab291080), expandable thumbnail](https://content.abcam.com/products/images/tau-phospho-t217-antibody-epr24654-110-ab291080--western-blot-img401962.jpg "Western blot - Anti-Tau (phospho T217) antibody [EPR24654-110] (ab291080)")
Western blot - Anti-Tau (phospho T217) antibody [EPR24654-110] (ab291080)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
All lanes: Western blot - Anti-Tau (phospho T217) antibody [EPR24654-110] (ab291080) at 1/1000 dilution
Lane 1: Mouse P7 hippocampus tissue lysate (Untreated membrane) at 20 µg
Lane 2: Mouse P7 hippocampus tissue lysate (Phosphatase treated membrane) at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Developed using the ECL technique.
Exposure time: 3min
![Immunocytochemistry/ Immunofluorescence - Anti-Tau (phospho T217) antibody [EPR24654-110] (ab291080), expandable thumbnail](https://content.abcam.com/products/images/tau-phospho-t217-antibody-epr24654-110-ab291080--immunocytochemistry-immunofluorescence-img401098.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Tau (phospho T217) antibody [EPR24654-110] (ab291080)")
Immunocytochemistry/ Immunofluorescence - Anti-Tau (phospho T217) antibody [EPR24654-110] (ab291080)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling Tau (phospho T217) with ab291080 at 1/2000 (0.29 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/mL dilution (Green). Confocal image showing the positive staining in mouse primary neuron, then the signal decreased after phosphatase treatment at 37°C for 2h. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. is observed. Anti-MAP2 antibody [HM-2] - Neuronal Marker ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/mL dilution.
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