AB288160

Anti-Tau (phospho T217) antibody [EPR24654-24]

20ul selling size
RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal TAU phospho T217 antibody. Suitable for Dot, sELISA, I-ELISA, WB and reacts with Synthetic peptide - Human, Human, Rat, Recombinant full length protein - Human samples. Cited in 1 publication.

8 Images

Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] (AB288160)

sELISA

Collaborator

Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] (AB288160)

This image data was created using the same antibody clone in a different buffer formulation (ab288167).

The concentration of pTau217 in matched human cerebrospinal fluid (CSF) and plasma samples from Alzheimer's disease patients (AD) and healthy controls (Ctrl) (n = 6) was quantified using the Singulex platform. Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (ab288167) was used in a ligand binding assay at 125 ng/well paired with a total Tau antibody. The effect size AD vs. Ctrl. is 14.7 for CSF samples.

The data was kindly provided by a collaborator.

Indirect ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] (AB288160)

I-ELISA

Lab

Indirect ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] (AB288160)

Indirect ELISA antibody dose-response curve using Human Tau non-phospho and Human Tau phospho T217 antigens. Antigen concentration was 1000 ng/mL. ab288160 was added at 0 - 1000 ng/mL. Samples were incubated with Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) secondary antibody at 1/2500 dilution.

sELISA

Collaborator

Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] (AB288160)

This image data was created using the same antibody clone in a different buffer formulation (ab288167).

The concentration of human pTau217 in human plasma samples from Alzheimer's disease patients (AD) and healthy controls (Ctrl) (n = 11) was quantified using the Singulex platform. Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (ab288167) was used in a ligand binding assay at 125 ng/well paired with a total Tau antibody. The effect size AD vs. Ctrl. is 4.5 for plasma samples.

The data was kindly provided by a collaborator.

sELISA

Collaborator

Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] (AB288160)

This image data was created using the same antibody clone in a different buffer formulation (ab288167).

The concentration of pTau217 in human soluble brain extracts from Alzheimer's disease patients (AD) and healthy controls (Ctrl) (n = 6) was quantified using the Singulex platform. Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (ab288167) was used in a ligand binding assay at 125 ng/well paired with a total Tau antibody. The effect size AD vs. Ctrl. is 3.2 for soluble brain extracts.

The data was kindly provided by a collaborator.

sELISA

Collaborator

Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] (AB288160)

This image data was created using the same antibody clone in a different buffer formulation (ab288167).

Standard Curve for human pTau (Analyte : human recombinant pTau protein at a concentration 0.5-1,000 pg/ml); using Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (ab288167) in a ligand binding assay at 125 ng/well paired with a total Tau antibody. The data was obtained on the Singulex platform.

The data was kindly provided by a collaborator.

sELISA

Collaborator

Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] (AB288160)

This image data was created using the same antibody clone in a different buffer formulation (ab288167).

Recovery for fluidic matrices (human CSF and plasma) at indicated dilutions using Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (ab288167) in a ligand binding assay at 125 ng/well paired with a total Tau antibody. The data was obtained on the Singulex platform.

The data was kindly provided by a collaborator.

Lab

Western blot - Anti-Tau (phospho T217) antibody [EPR24654-24] (AB288160)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/1000,000 dilution.

In Western blot, Anti-Tau antibody [EP2456Y] - Total protein control (ab76128)(1/1000)(55KDa).

All lanes:

Western blot - Anti-Tau (phospho T217) antibody [EPR24654-24] (ab288160) at 1/1000 dilution

Lane 1:

Rat P14 brain (Untreated membrane) at 20 µg

Lane 2:

Rat P5 brain (Untreated membrane) at 20 µg

Lane 3:

Rat P5 hippocampus (Untreated membrane) at 20 µg

Lane 4:

Rat P14 brain (Phosphatase treated membrane) at 20 µg

Lane 5:

Rat P5 brain (Phosphatase treated membrane) at 20 µg

Lane 6:

Rat P5 hippocampus (Phosphatase treated membrane) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 78 kDa

Observed band size: 50-80 kDa,36 kDa,55 kDa

false

Exposure time: 26s

Lab

Dot Blot - Anti-Tau (phospho T217) antibody [EPR24654-24] (AB288160)

Dot blot analysis using ab288160 at 1/1000 dilution, followed by ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) secondary antibody at 1/100000 dilution.

Lane 1 : Tau (phospho T217) peptide

Lene 2 : Tau (phospho T220) peptide

Lane 3 : Tau non-phosphoppotide

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

Carrier free

Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free
Carrier free

Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (Capture)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24654-24

Isotype

IgG

Carrier free

Reacts with

Human, Rat

Applications

I-ELISA, WB, Dot, sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The specificity of this antibody refers to P10636-8.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "Use at 31.25 ng/ml.", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "sELISA-species-checked": "notRecommended", "sELISA-species-dilution-info": "", "sELISA-species-notes": "", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Recombinant full length protein - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "Use at 31.25 ng/ml.", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Synthetic peptide - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "", "sELISA-species-checked": "notRecommended", "sELISA-species-dilution-info": "", "sELISA-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "Use at 31.25 ng/ml.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.

Biological function summary

Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.

Pathways

Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.

Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed : 21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed : 21985311, PubMed : 32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

See full target information MAPT pT217

Additional targets

MAPT pT217

Alternative Names

MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau, ptau, p tau, phospho tau, ptau 217, MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau, ptau, p tau, phospho tau, ptau 217

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Publications (1)

Recent publications for all applications. Explore the full listand refine your search

ACS nano 18:2117-2130 PubMed38117205

2023

High-Performance Plasma Biomarker Panel for Alzheimer's Disease Screening Using a Femtomolar-Level Label-Free Biosensing System.

Applications

Species

Human

Shicai Wang,Ruijun Deng,Zhiya Chen,Lili Huang,Yang Song,Dan Yuan,Yu Li,Haonan Liu,Fan Yang,Beiyuan Fan,Yun Xu,Zijian Zhao,Yanzhao Li,Yan Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Tau (phospho T217) antibody [EPR24654-24]

Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] (AB288160)

Indirect ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] (AB288160)

Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] (AB288160)

Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] (AB288160)

Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] (AB288160)

Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] (AB288160)

Western blot - Anti-Tau (phospho T217) antibody [EPR24654-24] (AB288160)

Dot Blot - Anti-Tau (phospho T217) antibody [EPR24654-24] (AB288160)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

Primary Antibodies

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Alternative Names

Publications (1)

Product promise