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AB288160

Anti-Tau (phospho T217) antibody [EPR24654-24]

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(1 Publication)

Rabbit Recombinant Monoclonal TAU phospho T217 antibody. Suitable for Dot, sELISA, I-ELISA and reacts with Synthetic peptide - Human, Human, Recombinant full length protein - Human samples. Cited in 1 publication.

View Alternative Names

MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

7 Images
Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] (AB288160)
  • sELISA

Collaborator

Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] (AB288160)

This image data was created using the same antibody clone in a different buffer formulation (ab288167).

Standard Curve for human pTau (Analyte : human recombinant pTau protein at a concentration 0.5-1,000 pg/ml); using Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (ab288167) in a ligand binding assay at 125 ng/well paired with a total Tau antibody. The data was obtained on the Singulex platform.

The data was kindly provided by a collaborator.

Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] (AB288160)
  • sELISA

Collaborator

Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] (AB288160)

This image data was created using the same antibody clone in a different buffer formulation (ab288167).

The concentration of human pTau217 in human plasma samples from Alzheimer's disease patients (AD) and healthy controls (Ctrl) (n = 11) was quantified using the Singulex platform. Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (ab288167) was used in a ligand binding assay at 125 ng/well paired with a total Tau antibody. The effect size AD vs. Ctrl. is 4.5 for plasma samples.

The data was kindly provided by a collaborator.

Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] (AB288160)
  • sELISA

Collaborator

Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] (AB288160)

This image data was created using the same antibody clone in a different buffer formulation (ab288167).

The concentration of pTau217 in human soluble brain extracts from Alzheimer's disease patients (AD) and healthy controls (Ctrl) (n = 6) was quantified using the Singulex platform. Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (ab288167) was used in a ligand binding assay at 125 ng/well paired with a total Tau antibody. The effect size AD vs. Ctrl. is 3.2 for soluble brain extracts.

The data was kindly provided by a collaborator.

Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] (AB288160)
  • sELISA

Collaborator

Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] (AB288160)

This image data was created using the same antibody clone in a different buffer formulation (ab288167).

Recovery for fluidic matrices (human CSF and plasma) at indicated dilutions using Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (ab288167) in a ligand binding assay at 125 ng/well paired with a total Tau antibody. The data was obtained on the Singulex platform.

The data was kindly provided by a collaborator.

Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] (AB288160)
  • sELISA

Collaborator

Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] (AB288160)

This image data was created using the same antibody clone in a different buffer formulation (ab288167).

The concentration of pTau217 in matched human cerebrospinal fluid (CSF) and plasma samples from Alzheimer's disease patients (AD) and healthy controls (Ctrl) (n = 6) was quantified using the Singulex platform. Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (ab288167) was used in a ligand binding assay at 125 ng/well paired with a total Tau antibody. The effect size AD vs. Ctrl. is 14.7 for CSF samples.

The data was kindly provided by a collaborator.

Indirect ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] (AB288160)
  • I-ELISA

Lab

Indirect ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] (AB288160)

Indirect ELISA antibody dose-response curve using Human Tau non-phospho and Human Tau phospho T217 antigens. Antigen concentration was 1000 ng/mL. ab288160 was added at 0 - 1000 ng/mL. Samples were incubated with Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) secondary antibody at 1/2500 dilution.

Dot Blot - Anti-Tau (phospho T217) antibody [EPR24654-24] (AB288160)
  • Dot

Lab

Dot Blot - Anti-Tau (phospho T217) antibody [EPR24654-24] (AB288160)

Dot blot analysis using ab288160 at 1/1000 dilution, followed by ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) secondary antibody at 1/100000 dilution.

Lane 1 : Tau (phospho T217) peptide

Lene 2 : Tau (phospho T220) peptide

Lane 3 : Tau non-phosphoppotide

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

  • Carrier free

    Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24654-24

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Dot, sELISA, I-ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The specificity of this antibody refers to P10636-8.

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Reactivity data

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AB318936

Human Tau (phospho T217) ELISA Kit - Extracellular

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We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.
Biological function summary

Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.

Pathways

Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.

Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The protein expressed by the MAPT gene promotes microtubule assembly and stability and might be involved in establishing and maintaining neuronal polarity. Its C-terminus binds axonal microtubules, and the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between the two. Axonal polarity is predetermined by MAPT localization within the neuronal cell's domain defined by the centrosome. The short isoforms allow cytoskeleton plasticity, whereas the longer isoforms may preferentially play a role in its stabilization. This supplementary information is collated from multiple sources and compiled automatically.
See full target information MAPT phospho T217

Additional targets

MAPT phospho T217,MAPT phospho T217
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

ACS nano 18:2117-2130 PubMed38117205

2023

High-Performance Plasma Biomarker Panel for Alzheimer's Disease Screening Using a Femtomolar-Level Label-Free Biosensing System.

Applications

Species

Human

Shicai Wang,Ruijun Deng,Zhiya Chen,Lili Huang,Yang Song,Dan Yuan,Yu Li,Haonan Liu,Fan Yang,Beiyuan Fan,Yun Xu,Zijian Zhao,Yanzhao Li,Yan Zhang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

Select an associated product type
Alternative Product
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Anti-Tau (phospho T217) antibody

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