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Rabbit Recombinant Monoclonal TAU phospho T217 antibody. Suitable for Dot, sELISA, I-ELISA and reacts with Synthetic peptide - Human, Human, Recombinant full length protein - Human samples. Cited in 1 publication.

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Images

Indirect ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] (AB288160), expandable thumbnail
  • Dot Blot - Anti-Tau (phospho T217) antibody [EPR24654-24] (AB288160), expandable thumbnail
  • Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] (AB288160), expandable thumbnail
  • Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] (AB288160), expandable thumbnail
  • Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] (AB288160), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PDotsELISAI-ELISA
Human
Not recommended
Expected
Tested
Expected
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Recombinant full length protein - Human
Not recommended
Not recommended
Tested
Not recommended
Synthetic peptide - Human
Not recommended
Tested
Not recommended
Tested

Not recommended
Not recommended

Species

Human, Mouse, Rat, Synthetic peptide - Human, Recombinant full length protein - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Synthetic peptide - Human

Dilution info
1/1000
Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Recombinant full length protein - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Recombinant full length protein - Human

Dilution info
-
Notes

Use at 31.25 ng/ml.

Species

Human

Dilution info
-
Notes

Use at 31.25 ng/ml.

Not recommended
Not recommended

Species

Mouse, Rat, Synthetic peptide - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Synthetic peptide - Human

Dilution info
-
Notes

Use at 31.25 ng/ml.

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Recombinant full length protein - Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed:21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed:21985311, PubMed:32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

Targets

MAPT phospho T217, MAPT phospho T217

Alternative names

Recommended products

Rabbit Recombinant Monoclonal TAU phospho T217 antibody. Suitable for Dot, sELISA, I-ELISA and reacts with Synthetic peptide - Human, Human, Recombinant full length protein - Human samples. Cited in 1 publication.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR24654-24

Purification technique

Affinity purification Protein A

Specificity

The specificity of this antibody refers to P10636-8.

Dissociation constant
3.12 x 10-11 M
Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.

Biological function summary

Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.

Pathways

Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.

Associated diseases and disorders

Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Indirect ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] (ab288160), expandable thumbnail

    Indirect ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] (ab288160)

    Indirect ELISA antibody dose-response curve using Human Tau non-phospho and Human Tau phospho T217 antigens. Antigen concentration was 1000 ng/mL. ab288160 was added at 0 - 1000 ng/mL. Samples were incubated with Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) secondary antibody at 1/2500 dilution.

  • Dot Blot - Anti-Tau (phospho T217) antibody [EPR24654-24] (ab288160), expandable thumbnail

    Dot Blot - Anti-Tau (phospho T217) antibody [EPR24654-24] (ab288160)

    Dot blot analysis using ab288160 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) secondary antibody at 1/100000 dilution.

    Lane 1: Tau (phospho T217) peptide

    Lene 2: Tau (phospho T220) peptide

    Lane 3: Tau non-phosphoppotide

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] (ab288160), expandable thumbnail

    Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] (ab288160)

    This image data was created using the same antibody clone in a different buffer formulation (ab288167).

    Standard Curve for human pTau (Analyte: human recombinant pTau protein at a concentration 0.5-1,000 pg/ml); using Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free ab288167) in a ligand binding assay at 125 ng/well paired with a total Tau antibody. The data was obtained on the Singulex platform.

    The data was kindly provided by a collaborator.

  • Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] (ab288160), expandable thumbnail

    Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] (ab288160)

    This image data was created using the same antibody clone in a different buffer formulation (ab288167).

    Recovery for fluidic matrices (human CSF and plasma) at indicated dilutions using Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free ab288167) in a ligand binding assay at 125 ng/well paired with a total Tau antibody. The data was obtained on the Singulex platform.

    The data was kindly provided by a collaborator.

  • Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] (ab288160), expandable thumbnail

    Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] (ab288160)

    This image data was created using the same antibody clone in a different buffer formulation (ab288167).

    The concentration of pTau217 in human soluble brain extracts from Alzheimer's disease patients (AD) and healthy controls (Ctrl) (n = 6) was quantified using the Singulex platform. Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free ab288167) was used in a ligand binding assay at 125 ng/well paired with a total Tau antibody. The effect size AD vs. Ctrl. is 3.2 for soluble brain extracts.

    The data was kindly provided by a collaborator.

  • Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] (ab288160), expandable thumbnail

    Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] (ab288160)

    This image data was created using the same antibody clone in a different buffer formulation (ab288167).

    The concentration of human pTau217 in human plasma samples from Alzheimer's disease patients (AD) and healthy controls (Ctrl) (n = 11) was quantified using the Singulex platform. Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free ab288167) was used in a ligand binding assay at 125 ng/well paired with a total Tau antibody. The effect size AD vs. Ctrl. is 4.5 for plasma samples.

    The data was kindly provided by a collaborator.

  • Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] (ab288160), expandable thumbnail

    Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] (ab288160)

    This image data was created using the same antibody clone in a different buffer formulation (ab288167).

    The concentration of pTau217 in matched human cerebrospinal fluid (CSF) and plasma samples from Alzheimer's disease patients (AD) and healthy controls (Ctrl) (n = 6) was quantified using the Singulex platform. Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free ab288167) was used in a ligand binding assay at 125 ng/well paired with a total Tau antibody. The effect size AD vs. Ctrl. is 14.7 for CSF samples.

    The data was kindly provided by a collaborator.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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