Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (ab288167) is a rabbit recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation detecting Tau in ELISA, Dot Blot. Suitable for Human.
- BSA, sodium azide, and glycerol-free for easy conjugation
- Biophysical QC for unrivalled batch-batch consistency
Images
![Indirect ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (AB288167), expandable thumbnail](https://content.abcam.com/products/images/tau-phospho-t217-antibody-epr24654-24-bsa-and-azide-free-ab288167--indirect-elisa-img170476.jpg/_jcr_content/renditions/webp-475.webp "Indirect ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (AB288167)")
![Dot Blot - Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (AB288167), expandable thumbnail](https://content.abcam.com/products/images/tau-phospho-t217-antibody-epr24654-24-bsa-and-azide-free-ab288167--dot-blot-img168239.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (AB288167)")
![Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (AB288167), expandable thumbnail](https://content.abcam.com/products/images/tau-phospho-t217-antibody-epr24654-24-bsa-and-azide-free-ab288167--sandwich-elisa-img170943.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (AB288167)")
![Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (AB288167), expandable thumbnail](https://content.abcam.com/products/images/tau-phospho-t217-antibody-epr24654-24-bsa-and-azide-free-ab288167--sandwich-elisa-img168838.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (AB288167)")
![Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (AB288167), expandable thumbnail](https://content.abcam.com/products/images/tau-phospho-t217-antibody-epr24654-24-bsa-and-azide-free-ab288167--sandwich-elisa-img167940.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (AB288167)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | Dot | sELISA | I-ELISA | |
|---|---|---|---|---|
| Human | Not recommended | Expected | Tested | Expected |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended |
| Recombinant full length protein - Human | Not recommended | Not recommended | Tested | Not recommended |
| Synthetic peptide - Human | Not recommended | Tested | Not recommended | Tested |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat, Mouse, Synthetic peptide - Human, Recombinant full length protein - Human | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Recombinant full length protein - Human | Dilution info - | Notes - |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human, Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Synthetic peptide - Human | Dilution info - | Notes - |
I-ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Recombinant full length protein - Human | Dilution info - | Notes - |
Target data
Function
The protein expressed by the MAPT gene promotes microtubule assembly and stability and might be involved in establishing and maintaining neuronal polarity. Its C-terminus binds axonal microtubules, and the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between the two. Axonal polarity is predetermined by MAPT localization within the neuronal cell's domain defined by the centrosome. The short isoforms allow cytoskeleton plasticity, whereas the longer isoforms may preferentially play a role in its stabilization. This supplementary information is collated from multiple sources and compiled automatically.
Additional Targets
MAPT phospho T217, MAPT phospho T217
Alternative names
MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau
Recommended products
Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (ab288167) is a rabbit recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation detecting Tau in ELISA, Dot Blot. Suitable for Human.
- BSA, sodium azide, and glycerol-free for easy conjugation
- Biophysical QC for unrivalled batch-batch consistency
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR24654-24
- Purification technique
- Affinity purification Protein A
- Specificity
The specificity of this antibody refers to P10636-8.
- Dissociation constant
- 3.12 x 10-11 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (ab288167) is a rabbit recombinant monoclonal antibody and is validated for use in ELISA, Dot Blot in Human samples.
Other related products
We have a range of other formats of antibody clone [EPR24654-24] also available for your convenience:
Anti-Tau (phospho T217) antibody [EPR24654-24] ab288160, Carrier free - ab288167, Carrier free - Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (Capture) ab314558
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.
- Biological function summary
Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.
- Pathways
Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3Β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.
- Associated diseases and disorders
Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Indirect ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (ab288167), expandable thumbnail](https://content.abcam.com/products/images/tau-phospho-t217-antibody-epr24654-24-bsa-and-azide-free-ab288167--indirect-elisa-img170476.jpg "Indirect ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (ab288167)")
Indirect ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (ab288167)
This image data was created using the same antibody clone in a different buffer formulation (Anti-Tau (phospho T217) antibody [EPR24654-24] ab288160).
Indirect ELISA antibody dose-response curve using Human Tau non-phospho and Human Tau phospho T217 antigens. Antigen concentration was 1000 ng/mL. Anti-Tau (phospho T217) antibody [EPR24654-24] ab288160 was added at 0 - 1000 ng/mL. Samples were incubated with Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) secondary antibody at 1/2500 dilution.
![Dot Blot - Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (ab288167), expandable thumbnail](https://content.abcam.com/products/images/tau-phospho-t217-antibody-epr24654-24-bsa-and-azide-free-ab288167--dot-blot-img168239.jpg "Dot Blot - Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (ab288167)")
Dot Blot - Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (ab288167)
This image data was created using the same antibody clone in a different buffer formulation (Anti-Tau (phospho T217) antibody [EPR24654-24] ab288160).
Dot blot analysis using Anti-Tau (phospho T217) antibody [EPR24654-24] ab288160 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) secondary antibody at 1/100000 dilution.
Lane 1: Tau (phospho T217) peptide
Lene 2: Tau (phospho T220) peptide
Lane 3: Tau non-phosphoppotide
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
![Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (ab288167), expandable thumbnail](https://content.abcam.com/products/images/tau-phospho-t217-antibody-epr24654-24-bsa-and-azide-free-ab288167--sandwich-elisa-img170943.jpg "Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (ab288167)")
Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (ab288167)
Standard Curve for human pTau (Analyte: human recombinant pTau protein at a concentration 0.5-1,000 pg/ml); using Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (ab288167) in a ligand binding assay at 125 ng/well paired with a total Tau antibody. The data was obtained on the Singulex platform.
The data was kindly provided by a collaborator.
![Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (ab288167), expandable thumbnail](https://content.abcam.com/products/images/tau-phospho-t217-antibody-epr24654-24-bsa-and-azide-free-ab288167--sandwich-elisa-img168838.jpg "Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (ab288167)")
Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (ab288167)
Recovery for fluidic matrices (human CSF and plasma) at indicated dilutions using Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (ab288167) in a ligand binding assay at 125 ng/well paired with a total Tau antibody. The data was obtained on the Singulex platform.
The data was kindly provided by a collaborator.
![Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (ab288167), expandable thumbnail](https://content.abcam.com/products/images/tau-phospho-t217-antibody-epr24654-24-bsa-and-azide-free-ab288167--sandwich-elisa-img167940.jpg "Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (ab288167)")
Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (ab288167)
The concentration of pTau217 in human soluble brain extracts from Alzheimer's disease patients (AD) and healthy controls (Ctrl) (n = 6) was quantified using the Singulex platform. Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (ab288167) was used in a ligand binding assay at 125 ng/well paired with a total Tau antibody. The effect size AD vs. Ctrl. is 3.2 for soluble brain extracts.
The data was kindly provided by a collaborator.
![Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (ab288167), expandable thumbnail](https://content.abcam.com/products/images/tau-phospho-t217-antibody-epr24654-24-bsa-and-azide-free-ab288167--sandwich-elisa-img179286.jpg "Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (ab288167)")
Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (ab288167)
The concentration of human pTau217 in human plasma samples from Alzheimer's disease patients (AD) and healthy controls (Ctrl) (n = 11) was quantified using the Singulex platform. Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (ab288167) was used in a ligand binding assay at 125 ng/well paired with a total Tau antibody. The effect size AD vs. Ctrl. is 4.5 for plasma samples.
The data was kindly provided by a collaborator.
![Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (ab288167), expandable thumbnail](https://content.abcam.com/products/images/tau-phospho-t217-antibody-epr24654-24-bsa-and-azide-free-ab288167--sandwich-elisa-img169640.jpg "Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (ab288167)")
Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (ab288167)
The concentration of pTau217 in matched human cerebrospinal fluid (CSF) and plasma samples from Alzheimer's disease patients (AD) and healthy controls (Ctrl) (n = 6) was quantified using the Singulex platform. Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (ab288167) was used in a ligand binding assay at 125 ng/well paired with a total Tau antibody. The effect size AD vs. Ctrl. is 14.7 for CSF samples.
The data was kindly provided by a collaborator.
![Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (ab288167), expandable thumbnail](https://content.abcam.com/products/images/tau-phospho-t217-antibody-epr24654-24-bsa-and-azide-free-ab288167--sandwich-elisa-img179286.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (ab288167)")
![Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (ab288167), expandable thumbnail](https://content.abcam.com/products/images/tau-phospho-t217-antibody-epr24654-24-bsa-and-azide-free-ab288167--sandwich-elisa-img169640.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (ab288167)")
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