AB288167

Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free

RabMAb
Recombinant
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Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (ab288167) is a rabbit recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation detecting Tau in ELISA, Dot Blot. Suitable for Human.

- BSA, sodium azide, and glycerol-free for easy conjugation
- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau, MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

7 Images

Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (AB288167)

sELISA

Collaborator

Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (AB288167)

Standard Curve for human pTau (Analyte : human recombinant pTau protein at a concentration 0.5-1,000 pg/ml); using Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (ab288167) in a ligand binding assay at 125 ng/well paired with a total Tau antibody. The data was obtained on the Singulex platform.

The data was kindly provided by a collaborator.

sELISA

Collaborator

Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (AB288167)

The concentration of human pTau217 in human plasma samples from Alzheimer's disease patients (AD) and healthy controls (Ctrl) (n = 11) was quantified using the Singulex platform. Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (ab288167) was used in a ligand binding assay at 125 ng/well paired with a total Tau antibody. The effect size AD vs. Ctrl. is 4.5 for plasma samples.

The data was kindly provided by a collaborator.

sELISA

Collaborator

Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (AB288167)

The concentration of pTau217 in human soluble brain extracts from Alzheimer's disease patients (AD) and healthy controls (Ctrl) (n = 6) was quantified using the Singulex platform. Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (ab288167) was used in a ligand binding assay at 125 ng/well paired with a total Tau antibody. The effect size AD vs. Ctrl. is 3.2 for soluble brain extracts.

The data was kindly provided by a collaborator.

Indirect ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (AB288167)

I-ELISA

Lab

Indirect ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (AB288167)

This image data was created using the same antibody clone in a different buffer formulation (ab288160).

Indirect ELISA antibody dose-response curve using Human Tau non-phospho and Human Tau phospho T217 antigens. Antigen concentration was 1000 ng/mL. ab288160 was added at 0 - 1000 ng/mL. Samples were incubated with Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) secondary antibody at 1/2500 dilution.

sELISA

Collaborator

Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (AB288167)

The concentration of pTau217 in matched human cerebrospinal fluid (CSF) and plasma samples from Alzheimer's disease patients (AD) and healthy controls (Ctrl) (n = 6) was quantified using the Singulex platform. Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (ab288167) was used in a ligand binding assay at 125 ng/well paired with a total Tau antibody. The effect size AD vs. Ctrl. is 14.7 for CSF samples.

The data was kindly provided by a collaborator.

sELISA

Collaborator

Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (AB288167)

Recovery for fluidic matrices (human CSF and plasma) at indicated dilutions using Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (ab288167) in a ligand binding assay at 125 ng/well paired with a total Tau antibody. The data was obtained on the Singulex platform.

The data was kindly provided by a collaborator.

Lab

Dot Blot - Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (AB288167)

This image data was created using the same antibody clone in a different buffer formulation (ab288160).

Dot blot analysis using ab288160 at 1/1000 dilution, followed by ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) secondary antibody at 1/100000 dilution.

Lane 1 : Tau (phospho T217) peptide

Lene 2 : Tau (phospho T220) peptide

Lane 3 : Tau non-phosphoppotide

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

Carrier free

Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (Capture)
Unconjugated

Anti-Tau (phospho T217) antibody [EPR24654-24]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24654-24

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

I-ELISA, Dot, sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The specificity of this antibody refers to P10636-8.

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Reactivity data

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Product details

What is this antibody validated in?
Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (ab288167) is a rabbit recombinant monoclonal antibody and is validated for use in ELISA, Dot Blot in Human samples.

Other related products
We have a range of other formats of antibody clone [EPR24654-24] also available for your convenience: ab288160, Carrier free - ab288167, Carrier free - ab314558

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.

Biological function summary

Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.

Pathways

Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3Β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.

Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed : 21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed : 21985311, PubMed : 32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

See full target information MAPT phospho T217

Additional targets

MAPT phospho T217

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free

Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (AB288167)

Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (AB288167)

Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (AB288167)

Indirect ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (AB288167)

Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (AB288167)

Sandwich ELISA - Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (AB288167)

Dot Blot - Anti-Tau (phospho T217) antibody [EPR24654-24] - BSA and Azide free (AB288167)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Complementary Products

Assay Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Product promise