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Rabbit Recombinant Monoclonal TAU phospho T231 antibody. Suitable for IHC-P, IP, WB, IHC-Fr, AP and reacts with Mouse, Rat, Human samples. Cited in 53 publications.


Images

Immunoprecipitation - Anti-Tau (phospho T231) antibody [EPR2488] (AB151559), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T231) antibody [EPR2488] (AB151559), expandable thumbnail
  • Western blot - Anti-Tau (phospho T231) antibody [EPR2488] (AB151559), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T231) antibody [EPR2488] (AB151559), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Tau (phospho T231) antibody [EPR2488] (AB151559), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBIHC-FrAP
Human
Tested
Expected
Tested
Tested
Tested
Mouse
Tested
Expected
Tested
Tested
Expected
Rat
Tested
Tested
Tested
Expected
Expected

Tested
Tested

Species

Mouse

Dilution info
1/500
Notes

For unpurified use at 1/200 dilution.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species

Rat

Dilution info
1/500
Notes

For unpurified use at 1/200 dilution.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species

Human

Dilution info
1/500
Notes

For unpurified use at 1/200 dilution.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species

Rat

Dilution info
1/20
Notes

For unpurified use at 1/50 dilution.

Expected
Expected

Species

Mouse, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info
1/1000 - 1/10000
Notes

-

Species

Rat

Dilution info
1/1000 - 1/10000
Notes

-

Species

Human

Dilution info
1/1000 - 1/10000
Notes

-

Tested
Tested

Species

Mouse

Dilution info
1/50
Notes

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

Species

Human

Dilution info
1/50
Notes

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info
-
Notes

Antibody concentration range - 4.17, 2.08, 1.04, 0.52, 0.26, 0 nM/mL

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

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Target data

Function

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed:21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed:21985311, PubMed:32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

Targets

MAPT phospho T231

Alternative names

Recommended products

Rabbit Recombinant Monoclonal TAU phospho T231 antibody. Suitable for IHC-P, IP, WB, IHC-Fr, AP and reacts with Mouse, Rat, Human samples. Cited in 53 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR2488

Purification technique

Affinity purification Protein A

Specificity

The specificity of this antibody refers to P10636-8.

Dissociation constant
1.26 x 10-11 M
Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This is the corresponding antibody for Anti-Tau (phospho T231) antibody [EPR2488] Blocking Peptide ab242015

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.

Biological function summary

Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.

Pathways

Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.

Associated diseases and disorders

Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

14 product images

  • Immunoprecipitation - Anti-Tau (phospho T231) antibody [EPR2488] (ab151559), expandable thumbnail

    Immunoprecipitation - Anti-Tau (phospho T231) antibody [EPR2488] (ab151559)

    ab151559 (purified) at 1:20 dilution (0.6μg) immunoprecipitating Tau in Rat cerebral cortex lysate. Lane 1 (input): Rat cerebral cortex lysate 10μg Lane 2 (+): ab151559 & Rat cerebral cortex lysateLane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab151559 inRat cerebral cortex lysateFor western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used for detection at 1:1000 dilution.Blocking and diluting buffer: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-Tau (phospho T231) antibody [EPR2488] (ab151559)

    Predicted band size: 78 kDa

    Observed band size: 50-70 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T231) antibody [EPR2488] (ab151559), expandable thumbnail
    Image courtesy of Carl Hobbs, Kings College London.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T231) antibody [EPR2488] (ab151559)

    IHC image of Tau (phospho T231) staining in human Alzheimer hippocampus formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with citrate buffer. The section was then incubated with unpurified ab151559 at 1/2000 dilution for 2 hours at 21°C. A biotin conjugated goat-anti-rabbit antibody was used as a secondary at 1/250. The section shows clear neurofibrillary tangles in a subset of neurons.

  • Western blot - Anti-Tau (phospho T231) antibody [EPR2488] (ab151559), expandable thumbnail

    Western blot - Anti-Tau (phospho T231) antibody [EPR2488] (ab151559)

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Tau (phospho T231) antibody [EPR2488] (ab151559) at 1/1000 dilution

    Lane 1: Human hippocampus tissue lysate at 15 µg

    Lane 2: Human hippocampus tissue lysate incubated with phosphatase at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 78 kDa

    Observed band size: 50-70 kDa

    Exposure time: 1s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T231) antibody [EPR2488] (ab151559), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T231) antibody [EPR2488] (ab151559)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat cerebrum tissue sections labeling Tau with Purified ab151559 at 1:500 dilution (0.24 μg/ml). Heat mediated antigen retrieval was performed using citrate (pH 6.0)

  • Immunohistochemistry (Frozen sections) - Anti-Tau (phospho T231) antibody [EPR2488] (ab151559), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Tau (phospho T231) antibody [EPR2488] (ab151559)

    Immunohistochemistry (Frozen sections) analysis of mouse cerebrum tissue sections labeling Tau with Purified ab151559 at 1/50 (1.5 μg/ml).Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T231) antibody [EPR2488] (ab151559), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T231) antibody [EPR2488] (ab151559)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse cerebrum tissue sections labeling Tau or Primary ab team with Purified ab151559 at 1:500 dilution (0.24 μg/ml). Heat mediated antigen retrieval was performed using citrate (pH 6.0)

  • Immunohistochemistry (Frozen sections) - Anti-Tau (phospho T231) antibody [EPR2488] (ab151559), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Tau (phospho T231) antibody [EPR2488] (ab151559)

    IHC image of Tau (phospho T231) staining in a section of frozen normal human Azheimer brain performed on a Leica BONDTM system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab151559, 1/1000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Western blot - Anti-Tau (phospho T231) antibody [EPR2488] (ab151559), expandable thumbnail

    Western blot - Anti-Tau (phospho T231) antibody [EPR2488] (ab151559)

    All lanes: Western blot - Anti-Tau (phospho T231) antibody [EPR2488] (ab151559) at 1/5000 dilution

    Lane 1: Rat cerebral cortex lysates at 15 µg

    Lane 2: Rat cerebral cortex lysates. Then the membrane was incubated with phosphatase. at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 78 kDa

    Observed band size: 50-70 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T231) antibody [EPR2488] (ab151559), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T231) antibody [EPR2488] (ab151559)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cerebrum tissue sections labeling Tau with Purified ab151559 at 1:500 dilution (0.24 μg/ml). Heat mediated antigen retrieval was performed using citrate (pH 6.0)

  • Western blot - Anti-Tau (phospho T231) antibody [EPR2488] (ab151559), expandable thumbnail

    Western blot - Anti-Tau (phospho T231) antibody [EPR2488] (ab151559)

    All lanes: Western blot - Anti-Tau (phospho T231) antibody [EPR2488] (ab151559) at 1/1000 dilution

    Lane 1: SH-SY5Y (human neuroblastoma cell line from bone marrow) cell lysate, untreated at 10 µg

    Lane 2: SH-SY5Y cell lysate, treated with sorbitol at 10 µg

    Predicted band size: 78 kDa

  • Western blot - Anti-Tau (phospho T231) antibody [EPR2488] (ab151559), expandable thumbnail

    Western blot - Anti-Tau (phospho T231) antibody [EPR2488] (ab151559)

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Anti-cardiac Troponin I antibody (ab1000) at 1/1000 dilution

    Lane 1: C57 (cerebral cortex) whole cell lysate at 15 µg

    Lane 2: C57 (cerebral cortex) whole cell lysate incubated with phosphatase at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 78 kDa

    Observed band size: 50-70 kDa

    Exposure time: 1s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T231) antibody [EPR2488] (ab151559), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T231) antibody [EPR2488] (ab151559)

    IHC image of Tau (phospho T231) staining in human Alzheimer hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6, epitope retrieval solution 1) for 20 minutes. The section was then incubated with unpurified ab151559, 1/200 dilution, for 15 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Western blot - Anti-Tau (phospho T231) antibody [EPR2488] (ab151559), expandable thumbnail

    Western blot - Anti-Tau (phospho T231) antibody [EPR2488] (ab151559)

    Blocking and dilution buffer: 5% NFDM/TBST.

    Western blot for Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] ab205606 is positioned on the right-hand side and serves as a control for the detection of the flag tag.

    All lanes: Western blot - Anti-Tau (phospho T231) antibody [EPR2488] (ab151559) at 1/1000 dilution

    Lane 1: 293T cells transfected with an empty vector containing a flag tag whole cell lysate at 20 µg

    Lane 2: 293T cells transfected with a human Tau expression vector containing a flag whole cell lysate at 20 µg

    Lane 3: 293T cells transfected with a human MAP2 expression vector containing a flag whole cell lysate at 20 µg

    Lane 4: 293T cells transfected with a human MAP4 expression vector containing a flag whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 50-100 kDa

    Exposure time: 1s

  • Affinity Purification - Anti-Tau (phospho T231) antibody [EPR2488] (ab151559), expandable thumbnail

    Affinity Purification - Anti-Tau (phospho T231) antibody [EPR2488] (ab151559)

    Biotinylated Human Tau (pT231) [0.3125 μg/ml] was loaded to SA biosensor on Fortebio RED96e Machine, then associate with recombinant Anti-Tau (phospho T231) antibody [EPR2488] in serial concentration points [4.17, 2.08, 1.04, 0.52, 0.26 nM/mL] by 2-fold dilution, next to dissociate in blank testing buffer [0.1% BSA in PBST (0.05%Tween-20)]. Calculated signals had already subtracted blank control, curve fitting using 1:1 binding model. Non-phospho Tau protein's association and dissociation were also showed in graph. KD(M) value of Anti-Tau (phospho T231) antibody [EPR2488] is 1.26E-11

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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