Name: Anti-Tau (phospho T231) antibody [EPR2488] - BSA and Azide free
SKU: ab156624
Rating: 5 (1 reviews)

Anti-Tau (phospho T231) antibody [EPR2488] - BSA and Azide free (ab156624) is a rabbit recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation detecting Tau in Western Blot, IP, IHC-P, IHC-Fr, ELISA. Suitable for Human, Mouse, Rat.

- BSA, sodium azide, and glycerol-free for easy conjugation

- Biophysical QC for unrivalled batch-batch consistency

Related conjugates and formulations

ab151559
Unconjugated
Anti-Tau (phospho T231) antibody [EPR2488]
ab312916
Conjugated
Alexa Fluor® 568 Anti-Tau (phospho T231) antibody [EPR2488]
ab321130
Conjugated
Alexa Fluor® 750 Anti-Tau (phospho T231) antibody [EPR2488]
ab303468
Conjugated
Alexa Fluor® 647 Anti-Tau (phospho T231) antibody [EPR2488]
ab306601
Conjugated
Alexa Fluor® 488 Anti-Tau (phospho T231) antibody [EPR2488]
ab313128
Conjugated
Alexa Fluor® 555 Anti-Tau (phospho T231) antibody [EPR2488]
ab311643
Conjugated
Alexa Fluor® 594 Anti-Tau (phospho T231) antibody [EPR2488]

Images

Immunoprecipitation - Anti-Tau (phospho T231) antibody [EPR2488] - BSA and Azide free (AB156624), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Constituents: PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	IP	WB	IHC-Fr	AP	I-ELISA
Human	Tested	Expected	Expected	Tested	Tested	Expected
Mouse	Tested	Expected	Expected	Tested	Expected	Expected
Rat	Tested	Tested	Expected	Expected	Expected	Expected
Synthetic peptide - Human	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended	Tested

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Synthetic peptide - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Rat	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Human	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Synthetic peptide - Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Synthetic peptide - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Species Human	Dilution info -	Notes Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Synthetic peptide - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Antibody concentration range - 4.17, 2.08, 1.04, 0.52, 0.26, 0 nM/mL

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Synthetic peptide - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Synthetic peptide - Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

Select an associated product type

11 products for Alternative Product

4 products for Alternative Version

Target data

See full target information MAPT phospho T231

Function

The protein expressed by the MAPT gene promotes microtubule assembly and stability and might be involved in establishing and maintaining neuronal polarity. Its C-terminus binds axonal microtubules, and the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between the two. Axonal polarity is predetermined by MAPT localization within the neuronal cell's domain defined by the centrosome. The short isoforms allow cytoskeleton plasticity, whereas the longer isoforms may preferentially play a role in its stabilization. This supplementary information is collated from multiple sources and compiled automatically.

Additional Targets

MAPT phospho T231

Alternative names

MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Constituents: PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR2488
Purification technique: Affinity purification Protein A
Specificity: The specificity of this antibody refers to P10636-8.
Dissociation constant: 1.26 x 10^-11 M
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: Do Not Freeze

Notes

What is this antibody validated in?

Anti-Tau (phospho T231) antibody [EPR2488] - BSA and Azide free (ab156624) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), ELISA in Human, Mouse, Rat samples.

What is the molecular weight of Tau?

Anti-Tau (phospho T231) [EPR2488] - BSA and Azide free (ab156624) specifically detects a band for Tau (UniProt: P10636) at a molecular weight of 46kDa.

Other related products

We have a range of other formats of antibody clone [EPR2488] also available for your convenience:
ab151559, Carrier free - ab156624, Alexa Fluor® 647 - ab303468, Alexa Fluor® 594 - ab311643, Alexa Fluor® 568 - ab312916, Alexa Fluor® 555 - ab313128, Carrier free - ab319089, Alexa Fluor® 750 - ab321130

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.

Biological function summary

Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.

Pathways

Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3Β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.

Associated diseases and disorders

Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

Immunoprecipitation - Anti-Tau (phospho T231) antibody [EPR2488] - BSA and Azide free (ab156624)
Anti-Tau (phospho T231) antibody [EPR2488] ab151559 (purified) at 1:20 dilution (0.6μg) immunoprecipitating Tau in Rat cerebral cortex lysate.
Lane 1 (input): Rat cerebral cortex lysate 10μg
Lane 2 (+): Anti-Tau (phospho T231) antibody [EPR2488] ab151559 & Rat cerebral cortex lysate
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Tau (phospho T231) antibody [EPR2488] ab151559 inRat cerebral cortex lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Tau (phospho T231) antibody [EPR2488] ab151559).
All lanes: Immunoprecipitation - Anti-Tau (phospho T231) antibody [EPR2488] (Anti-Tau (phospho T231) antibody [EPR2488] ab151559)
Predicted band size: 78 kDa
Observed band size: 50-70 kDa
Image courtesy of Carl Hobbs, Kings College London.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T231) antibody [EPR2488] - BSA and Azide free (ab156624)
IHC image of Tau (phospho T231) staining in human Alzheimer hippocampus formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with citrate buffer. The section was then incubated with unpurified Anti-Tau (phospho T231) antibody [EPR2488] ab151559 at 1/2000 dilution for 2 hours at 21ºC. A biotin conjugated goat-anti-rabbit antibody was used as a secondary at 1/250. The section shows clear neurofibrillary tangles in a subset of neurons.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Tau (phospho T231) antibody [EPR2488] ab151559).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T231) antibody [EPR2488] - BSA and Azide free (ab156624)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat cerebrum tissue sections labeling Tau with Purified Anti-Tau (phospho T231) antibody [EPR2488] ab151559 at 1:500 dilution (0.24 µg/ml). Heat mediated antigen retrieval was performed using citrate (pH 6.0)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Tau (phospho T231) antibody [EPR2488] ab151559).
Immunohistochemistry (Frozen sections) - Anti-Tau (phospho T231) antibody [EPR2488] - BSA and Azide free (ab156624)
Immunohistochemistry (Frozen sections) analysis of mouse cerebrum tissue sections labeling Tau with Purified Anti-Tau (phospho T231) antibody [EPR2488] ab151559 at 1/50 (1.5 μg/ml).Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Tau (phospho T231) antibody [EPR2488] ab151559).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T231) antibody [EPR2488] - BSA and Azide free (ab156624)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse cerebrum tissue sections labeling Tau or Primary ab team with Purified Anti-Tau (phospho T231) antibody [EPR2488] ab151559 at 1:500 dilution (0.24 µg/ml). Heat mediated antigen retrieval was performed using citrate (pH 6.0)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Tau (phospho T231) antibody [EPR2488] ab151559).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T231) antibody [EPR2488] - BSA and Azide free (ab156624)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cerebrum tissue sections labeling Tau with Purified Anti-Tau (phospho T231) antibody [EPR2488] ab151559 at 1:500 dilution (0.24 µg/ml). Heat mediated antigen retrieval was performed using citrate (pH 6.0)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Tau (phospho T231) antibody [EPR2488] ab151559).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T231) antibody [EPR2488] - BSA and Azide free (ab156624)
IHC image of Tau (phospho T231) staining in human Alzheimer hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6, epitope retrieval solution 1) for 20 minutes. The section was then incubated with unpurified Anti-Tau (phospho T231) antibody [EPR2488] ab151559, 1/200 dilution, for 15 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Tau (phospho T231) antibody [EPR2488] ab151559).
Affinity Purification - Anti-Tau (phospho T231) antibody [EPR2488] - BSA and Azide free (ab156624)
Biotinylated Human Tau (pT231) [0.3125 μg/ml] was loaded to SA biosensor on Fortebio RED96e Machine, then associate with recombinant Anti-Tau (phospho T231) antibody [EPR2488] in serial concentration points [4.17, 2.08, 1.04, 0.52, 0.26 nM/mL] by 2-fold dilution, next to dissociate in blank testing buffer [0.1% BSA in PBST (0.05%Tween-20)]. Calculated signals had already subtracted blank control, curve fitting using 1:1 binding model. Non-phospho Tau protein's association and dissociation were also showed in graph. KD(M) value of Anti-Tau (phospho T231) antibody [EPR2488] is 1.26E-11
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Tau (phospho T231) antibody [EPR2488] ab151559).
Western blot - Anti-Tau (phospho T231) antibody [EPR2488] - BSA and Azide free (ab156624)
Blocking and dilution buffer: 5% NFDM/TBST.
Western blot for Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] ab205606 is positioned on the right-hand side and serves as a control for the detection of the flag tag.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Tau (phospho T231) antibody [EPR2488] ab151559).
All lanes: Western blot - Anti-Tau (phospho T231) antibody [EPR2488] (Anti-Tau (phospho T231) antibody [EPR2488] ab151559) at 1/1000 dilution
Lane 1: 293T cells transfected with an empty vector containing a flag tag whole cell lysate at 20 µg
Lane 2: 293T cells transfected with a human Tau expression vector containing a flag whole cell lysate at 20 µg
Lane 3: 293T cells transfected with a human MAP2 expression vector containing a flag whole cell lysate at 20 µg
Lane 4: 293T cells transfected with a human MAP4 expression vector containing a flag whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 50-100 kDa
Exposure time: 1s
Indirect ELISA - Anti-Tau (phospho T231) antibody [EPR2488] - BSA and Azide free (ab156624)
This data was developed using Anti-Tau (phospho T231) antibody [EPR2488] ab151559, the same antibody clone in a different buffer formulation.
Indirect ELISA analysis of Anti-Tau (phospho T231) antibody [EPR2488] ab151559 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution.
Antigen: Human Tau (phospho T231) peptide, Human Tau non-phospho peptide
Antigen concentration: 1000 ng/ml.