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AB324765

Anti-Tau (phospho T50) antibody [EPR29148-44] - BSA and Azide free

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Rabbit Recombinant Monoclonal TAU phospho T50 antibody. Carrier free. Suitable for Dot, WB, I-ELISA and reacts with Synthetic peptide, Human, Transfected cell lysate - Human samples.

View Alternative Names

MAPTL, MTBT1, TAU, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

5 Images
Western blot - Anti-Tau (phospho T50) antibody [EPR29148-44] - BSA and Azide free (AB324765)
  • WB

Supplier Data

Western blot - Anti-Tau (phospho T50) antibody [EPR29148-44] - BSA and Azide free (AB324765)

This data was developed using ab322143, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot Anti-Tau - C-terminal antibody [EPR28785-33] - (ab316121) staining at 1/1000 dilution.

Actual observed bands 35-70kDa.

All lanes:

Western blot - Anti-Tau (phospho T50) antibody [EPR29148-44] (<a href='/en-us/products/primary-antibodies/tau-phospho-t50-antibody-epr29148-44-ab322143'>ab322143</a>) at 1/1000 dilution

Lane 1:

Human brain tissue lysate (untreated membrane) at 20 µg

Lane 2:

Human brain tissue lysate (alkaline phosphatase treated membrane) at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 35-70 kDa,50-70 kDa

false

Exposure time: 92s

Western blot - Anti-Tau (phospho T50) antibody [EPR29148-44] - BSA and Azide free (AB324765)
  • WB

Supplier Data

Western blot - Anti-Tau (phospho T50) antibody [EPR29148-44] - BSA and Azide free (AB324765)

This data was developed using ab322143, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : kidney spleen (PMID : 24386422; PMID : 24309898).

In Western blot Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lane 1 : 180 seconds; Lanes 2-4 : 26 seconds.

Actual observed bands 35-70kDa.

All lanes:

Western blot - Anti-Tau (phospho T50) antibody [EPR29148-44] (<a href='/en-us/products/primary-antibodies/tau-phospho-t50-antibody-epr29148-44-ab322143'>ab322143</a>) at 1/1000 dilution

Lane 1:

Human brainstem tissue lysate at 20 µg

Lane 2:

Human brain tissue lysate at 20 µg

Lane 3:

Human kidney tissue lysate at 20 µg

Lane 4:

Human spleen tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 35-70 kDa,36 kDa

false

Indirect ELISA - Anti-Tau (phospho T50) antibody [EPR29148-44] - BSA and Azide free (AB324765)
  • I-ELISA

Supplier Data

Indirect ELISA - Anti-Tau (phospho T50) antibody [EPR29148-44] - BSA and Azide free (AB324765)

This data was developed using ab322143, the same antibody clone in a different buffer formulation.

Indirect ELISA analysis of ab322143 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1 : 2500 dilution.

Antigen : Tau (phospho T50) peptideTau non-phospho peptideTau (phospho T52) peptide.

Antigen concentration : 1000 ng/ml

Western blot - Anti-Tau (phospho T50) antibody [EPR29148-44] - BSA and Azide free (AB324765)
  • WB

Supplier Data

Western blot - Anti-Tau (phospho T50) antibody [EPR29148-44] - BSA and Azide free (AB324765)

This data was developed using ab322143, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The identity of the bands higher than 100 kDa are unknown.

In Western blot Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

In Western blot Anti-Tau - C-terminal antibody [EPR28785-33] - (ab316121) staining at 1/1000 dilution.

Actual observed bands 50-70kDa.

All lanes:

Western blot - Anti-Tau (phospho T50) antibody [EPR29148-44] (<a href='/en-us/products/primary-antibodies/tau-phospho-t50-antibody-epr29148-44-ab322143'>ab322143</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with a human Tau (T50A mutation) expression vector containing a myc-His-tag® treated with 100nM Calyculin A for 30min, whole cell lysate (untreated membrane) at 20 µg

Lane 2:

293T cells transfected with a human wild-type Tau expression vector containing a myc-His-tag® treated with 100nM Calyculin A for 30min, whole cell lysate (untreated membrane) at 20 µg

Lane 3:

293T cells transfected with a human wild-type Tau expression vector containing a myc-His-tag® treated with 100nM Calyculin A for 30min, whole cell lysate (alkaline phosphatase treated membrane) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 50-70 kDa

false

Exposure time: 8s

Dot Blot - Anti-Tau (phospho T50) antibody [EPR29148-44] - BSA and Azide free (AB324765)
  • Dot

Supplier Data

Dot Blot - Anti-Tau (phospho T50) antibody [EPR29148-44] - BSA and Azide free (AB324765)

This data was developed using ab322143, the same antibody clone in a different buffer formulation.

Dot blot analysis of Tau (phospho T50) using ab322143 at 1 : 1000 (0.499 ug/ml) followed by a Goat Anti-Rabbit IgG (H+L) Peroxidase conjugated (ab97051) at 1 : 100000 dilution.

Lane1 : Tau (phospho T50) peptide
Lane2 : Tau non-phospho peptide
Lane3 : Tau (phospho T52) peptide

Exposure time : 125 seconds.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Dot Blot - Anti-Tau (phospho T50) antibody [EPR29148-44] (<a href='/en-us/products/primary-antibodies/tau-phospho-t50-antibody-epr29148-44-ab322143'>ab322143</a>) at 1/1000 dilution

Lane 1:

Tau (phospho T50) peptide

Lane 2:

Tau non-phospho peptide

Lane 3:

Tau (phospho T52) peptide

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 125s

  • Unconjugated

    Anti-Tau (phospho T50) antibody [EPR29148-44]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR29148-44

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, I-ELISA, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody doesn't work in cell lines.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Synthetic peptide": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Transfected cell lysate - Human": { "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

ab324765 is the carrier-free version of ab322143.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed : 21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed : 21985311, PubMed : 32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
See full target information MAPT phospho T50
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com