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Rabbit Recombinant Monoclonal TAU phospho T69 antibody. Suitable for WB, IHC-P, I-ELISA, Dot and reacts with Human, Mouse, Synthetic peptide, Transfected cell lysate - Human samples.

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Images

Dot Blot - Anti-Tau (phospho T69) antibody [EPR29149-92] (AB322410), expandable thumbnail
  • Indirect ELISA - Anti-Tau (phospho T69) antibody [EPR29149-92] (AB322410), expandable thumbnail
  • Western blot - Anti-Tau (phospho T69) antibody [EPR29149-92] (AB322410), expandable thumbnail
  • Western blot - Anti-Tau (phospho T69) antibody [EPR29149-92] (AB322410), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T69) antibody [EPR29149-92] (AB322410), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PI-ELISADot
Human
Tested
Tested
Expected
Expected
Mouse
Tested
Not recommended
Expected
Expected
Synthetic peptide
Not recommended
Not recommended
Tested
Not recommended
Transfected cell lysate - Human
Not recommended
Not recommended
Not recommended
Tested

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Species

Mouse

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide, Transfected cell lysate - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/2000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Synthetic peptide

Dilution info

-

Notes

-

Species

Transfected cell lysate - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Synthetic peptide

Dilution info

1000 ng/mL

Notes

-

Expected
Expected

Species

Human, Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Transfected cell lysate - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Transfected cell lysate - Human

Dilution info

1/1000

Notes

-

Expected
Expected

Species

Human, Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Target data

Function

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed:21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed:21985311, PubMed:32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

Targets

Mapt

Alternative names

Rabbit Recombinant Monoclonal TAU phospho T69 antibody. Suitable for WB, IHC-P, I-ELISA, Dot and reacts with Human, Mouse, Synthetic peptide, Transfected cell lysate - Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogens
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR29149-92

Purification technique

Affinity purification Protein A

Specificity

This product do not work in cell lines, and is unsuitable for mouse IHC.
This antibody recognized mouse Tau (phospho T58), corresponding to human Tau (phospho T69).

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Dot Blot - Anti-Tau (phospho T69) antibody [EPR29149-92] (ab322410), expandable thumbnail

    Dot Blot - Anti-Tau (phospho T69) antibody [EPR29149-92] (ab322410)

    Dot blot analysis of Tau (phospho T69) using ab322410 at 1:1000 (0.516 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.

    Lane1: 293T cells transfected with a human Tau (phospho T69) expression vector containing a myc-His-tag®, whole cell lysate

    Lane2: 293T cells transfected with a empty vector containing a myc-His-tag®, whole cell lysate

    Lane3: 293T cells transfected with a human mutant Tau (T69A) expression vector containing a myc-His-tag®, whole cell lysate

    Exposure time: 180 seconds.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This antibody specifically recognizes human Tau (phospho T69).

    #In Dot Blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

    All lanes: Dot Blot - Anti-Tau (phospho T69) antibody [EPR29149-92] (ab322410) at 1/1000 dilution

    Lane 1: 293T cells transfected with a human Tau (phospho T69) expression vector containing a myc-His-tag®, whole cell lysate

    Lane 2: 293T cells transfected with a empty vector containing a myc-His-tag®, whole cell lysate

    Lane 3: 293T cells transfected with a human mutant Tau (T69A) expression vector containing a myc-His-tag®, whole cell lysate

    Secondary

    All lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Exposure time: 180s

  • Indirect ELISA - Anti-Tau (phospho T69) antibody [EPR29149-92] (ab322410), expandable thumbnail

    Indirect ELISA - Anti-Tau (phospho T69) antibody [EPR29149-92] (ab322410)

    Indirect ELISA analysis of ab322410 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution.

    Antigen: Tau (phospho T69) peptide, Tau non-phospho, Tau (phospho T71).

    Antigen concentration: 1000 ng/ml

    This antibody is recommended in mouse ELISA as Human Tau (phospho T69) peptide has 100% homology with mouse Tau (phospho T58).

  • Western blot - Anti-Tau (phospho T69) antibody [EPR29149-92] (ab322410), expandable thumbnail

    Western blot - Anti-Tau (phospho T69) antibody [EPR29149-92] (ab322410)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: Mouse liver.

    This antibody recognized mouse Tau (phospho T58), corresponding to human Tau (phospho T69).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-Tau (phospho T69) antibody [EPR29149-92] (ab322410) at 1/1000 dilution

    Lane 1: Mouse brain tissue lysate (untreated membrane) at 20 µg

    Lane 2: Mouse hippocampus tissue lysate (untreated membrane) at 20 µg

    Lane 3: Mouse liver tissue lysate (untreated membrane) at 20 µg

    Lane 4: Mouse brain tissue lysate (alkaline phosphatase treated membrane) at 20 µg

    Lane 5: Mouse hippocampus tissue lysate (alkaline phosphatase treated membrane) at 20 µg

    Lane 6: Mouse liver tissue lysate (alkaline phosphatase treated membrane) at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 50-70 kDa, 36 kDa

    Exposure time: 180s

  • Western blot - Anti-Tau (phospho T69) antibody [EPR29149-92] (ab322410), expandable thumbnail

    Western blot - Anti-Tau (phospho T69) antibody [EPR29149-92] (ab322410)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: Human liver.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-Tau (phospho T69) antibody [EPR29149-92] (ab322410) at 1/1000 dilution

    Lane 1: Human hippocampus tissue lysate (untreated membrane) at 20 µg

    Lane 2: Human liver tissue lysate (untreated membrane) at 20 µg

    Lane 3: Human hippocampus tissue lysate (alkaline phosphatase treated membrane) at 20 µg

    Lane 4: Human liver tissue lysate (alkaline phosphatase treated membrane) at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 50-70 kDa, 36 kDa

    Exposure time: 37s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T69) antibody [EPR29149-92] (ab322410), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T69) antibody [EPR29149-92] (ab322410)

    Immunohistochemical analysis of paraffin-embedded Human Alzheimer's disease brain tissue labeling Tau (phospho T69) with ab322410 at 1/2000 (0.258 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on plaque of human Alzheimer's disease brain without alkaline phosphatase treatment (image A). No signal was detected when tissues were treated with alkaline phosphatase (image B).

    The section was incubated with ab322410 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T69) antibody [EPR29149-92] (ab322410), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T69) antibody [EPR29149-92] (ab322410)

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Tau (phospho T69) with ab322410 at 1/2000 (0.258 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on plaque of human cerebrum without alkaline phosphatase treatment (image A). No signal was detected when tissues were treated with alkaline phosphatase (image B).

    The section was incubated with ab322410 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T69) antibody [EPR29149-92] (ab322410), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T69) antibody [EPR29149-92] (ab322410)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Tau (phospho T69) with ab322410 at 1/2000 (0.258 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: No staining in human liver.

    The section was incubated with ab322410 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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