Rabbit Recombinant Monoclonal TAU phospho T69 antibody. Suitable for WB, IHC-P, I-ELISA, Dot and reacts with Human, Mouse, Synthetic peptide, Transfected cell lysate - Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
WB | IHC-P | I-ELISA | Dot | |
---|---|---|---|---|
Human | Tested | Tested | Expected | Expected |
Mouse | Tested | Not recommended | Expected | Expected |
Synthetic peptide | Not recommended | Not recommended | Tested | Not recommended |
Transfected cell lysate - Human | Not recommended | Not recommended | Not recommended | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Species Mouse | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Synthetic peptide, Transfected cell lysate - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/2000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Synthetic peptide | Dilution info - | Notes - |
Species Transfected cell lysate - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Synthetic peptide | Dilution info 1000 ng/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Transfected cell lysate - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Transfected cell lysate - Human | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed:21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed:21985311, PubMed:32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
Mapt
MAPTL, MTBT1, TAU, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau
Rabbit Recombinant Monoclonal TAU phospho T69 antibody. Suitable for WB, IHC-P, I-ELISA, Dot and reacts with Human, Mouse, Synthetic peptide, Transfected cell lysate - Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EPR29149-92
Affinity purification Protein A
This product do not work in cell lines, and is unsuitable for mouse IHC.
This antibody recognized mouse Tau (phospho T58), corresponding to human Tau (phospho T69).
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Dot blot analysis of Tau (phospho T69) using ab322410 at 1:1000 (0.516 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.
Lane1: 293T cells transfected with a human Tau (phospho T69) expression vector containing a myc-His-tag®, whole cell lysate
Lane2: 293T cells transfected with a empty vector containing a myc-His-tag®, whole cell lysate
Lane3: 293T cells transfected with a human mutant Tau (T69A) expression vector containing a myc-His-tag®, whole cell lysate
Exposure time: 180 seconds.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This antibody specifically recognizes human Tau (phospho T69).
#In Dot Blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
All lanes: Dot Blot - Anti-Tau (phospho T69) antibody [EPR29149-92] (ab322410) at 1/1000 dilution
Lane 1: 293T cells transfected with a human Tau (phospho T69) expression vector containing a myc-His-tag®, whole cell lysate
Lane 2: 293T cells transfected with a empty vector containing a myc-His-tag®, whole cell lysate
Lane 3: 293T cells transfected with a human mutant Tau (T69A) expression vector containing a myc-His-tag®, whole cell lysate
All lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Exposure time: 180s
Indirect ELISA analysis of ab322410 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution.
Antigen: Tau (phospho T69) peptide, Tau non-phospho, Tau (phospho T71).
Antigen concentration: 1000 ng/ml
This antibody is recommended in mouse ELISA as Human Tau (phospho T69) peptide has 100% homology with mouse Tau (phospho T58).
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: Mouse liver.
This antibody recognized mouse Tau (phospho T58), corresponding to human Tau (phospho T69).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-Tau (phospho T69) antibody [EPR29149-92] (ab322410) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate (untreated membrane) at 20 µg
Lane 2: Mouse hippocampus tissue lysate (untreated membrane) at 20 µg
Lane 3: Mouse liver tissue lysate (untreated membrane) at 20 µg
Lane 4: Mouse brain tissue lysate (alkaline phosphatase treated membrane) at 20 µg
Lane 5: Mouse hippocampus tissue lysate (alkaline phosphatase treated membrane) at 20 µg
Lane 6: Mouse liver tissue lysate (alkaline phosphatase treated membrane) at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 50-70 kDa, 36 kDa
Exposure time: 180s
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: Human liver.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-Tau (phospho T69) antibody [EPR29149-92] (ab322410) at 1/1000 dilution
Lane 1: Human hippocampus tissue lysate (untreated membrane) at 20 µg
Lane 2: Human liver tissue lysate (untreated membrane) at 20 µg
Lane 3: Human hippocampus tissue lysate (alkaline phosphatase treated membrane) at 20 µg
Lane 4: Human liver tissue lysate (alkaline phosphatase treated membrane) at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 50-70 kDa, 36 kDa
Exposure time: 37s
Immunohistochemical analysis of paraffin-embedded Human Alzheimer's disease brain tissue labeling Tau (phospho T69) with ab322410 at 1/2000 (0.258 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on plaque of human Alzheimer's disease brain without alkaline phosphatase treatment (image A). No signal was detected when tissues were treated with alkaline phosphatase (image B).
The section was incubated with ab322410 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Tau (phospho T69) with ab322410 at 1/2000 (0.258 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on plaque of human cerebrum without alkaline phosphatase treatment (image A). No signal was detected when tissues were treated with alkaline phosphatase (image B).
The section was incubated with ab322410 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Tau (phospho T69) with ab322410 at 1/2000 (0.258 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: No staining in human liver.
The section was incubated with ab322410 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com