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AB324840

Anti-Tau (phospho T69) antibody [EPR29149-92] - BSA and Azide free

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Rabbit Recombinant Monoclonal TAU phospho T69 antibody. Carrier free. Suitable for IHC-P, I-ELISA, Dot, WB and reacts with Human, Synthetic peptide, Mouse, Transfected cell lysate - Human samples.

View Alternative Names

MAPTL, MTBT1, TAU, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau, Mtapt, Tau, Mapt, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau, Big tau

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T69) antibody [EPR29149-92] - BSA and Azide free (AB324840)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T69) antibody [EPR29149-92] - BSA and Azide free (AB324840)

This data was developed using ab322410, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human Alzheimer's disease brain tissue labeling Tau (phospho T69) with ab322410 at 1/2000 (0.258 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on plaque of human Alzheimer's disease brain without alkaline phosphatase treatment (image A). No signal was detected when tissues were treated with alkaline phosphatase (image B).
The section was incubated with ab322410 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T69) antibody [EPR29149-92] - BSA and Azide free (AB324840)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T69) antibody [EPR29149-92] - BSA and Azide free (AB324840)

This data was developed using ab322410, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Tau (phospho T69) with ab322410 at 1/2000 (0.258 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining in human liver.
The section was incubated with ab322410 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T69) antibody [EPR29149-92] - BSA and Azide free (AB324840)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T69) antibody [EPR29149-92] - BSA and Azide free (AB324840)

This data was developed using ab322410, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Tau (phospho T69) with ab322410 at 1/2000 (0.258 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on plaque of human cerebrum without alkaline phosphatase treatment (image A). No signal was detected when tissues were treated with alkaline phosphatase (image B).
The section was incubated with ab322410 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Western blot - Anti-Tau (phospho T69) antibody [EPR29149-92] - BSA and Azide free (AB324840)
  • WB

Supplier Data

Western blot - Anti-Tau (phospho T69) antibody [EPR29149-92] - BSA and Azide free (AB324840)

This data was developed using ab322410, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : Human liver.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Tau (phospho T69) antibody [EPR29149-92] (<a href='/en-us/products/primary-antibodies/tau-phospho-t69-antibody-epr29149-92-ab322410'>ab322410</a>) at 1/1000 dilution

Lane 1:

Human hippocampus tissue lysate (untreated membrane) at 20 µg

Lane 2:

Human liver tissue lysate (untreated membrane) at 20 µg

Lane 3:

Human hippocampus tissue lysate (alkaline phosphatase treated membrane) at 20 µg

Lane 4:

Human liver tissue lysate (alkaline phosphatase treated membrane) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 50-70 kDa,36 kDa

false

Exposure time: 37s

Dot Blot - Anti-Tau (phospho T69) antibody [EPR29149-92] - BSA and Azide free (AB324840)
  • Dot

Supplier Data

Dot Blot - Anti-Tau (phospho T69) antibody [EPR29149-92] - BSA and Azide free (AB324840)

This data was developed using ab322410, the same antibody clone in a different buffer formulation.

Dot blot analysis of Tau (phospho T69) using ab322410 at 1 : 1000 (0.516 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Lane1 : 293T cells transfected with a human Tau (phospho T69) expression vector containing a myc-His-tag®, whole cell lysate
Lane2 : 293T cells transfected with a empty vector containing a myc-His-tag®, whole cell lysate
Lane3 : 293T cells transfected with a human mutant Tau (T69A) expression vector containing a myc-His-tag®, whole cell lysate

Exposure time : 180 seconds.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody specifically recognizes human Tau (phospho T69).

In Dot Blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Dot Blot - Anti-Tau (phospho T69) antibody [EPR29149-92] (<a href='/en-us/products/primary-antibodies/tau-phospho-t69-antibody-epr29149-92-ab322410'>ab322410</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with a human Tau (phospho T69) expression vector containing a myc-His-tag®, whole cell lysate

Lane 2:

293T cells transfected with a empty vector containing a myc-His-tag®, whole cell lysate

Lane 3:

293T cells transfected with a human mutant Tau (T69A) expression vector containing a myc-His-tag®, whole cell lysate

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s

Western blot - Anti-Tau (phospho T69) antibody [EPR29149-92] - BSA and Azide free (AB324840)
  • WB

Supplier Data

Western blot - Anti-Tau (phospho T69) antibody [EPR29149-92] - BSA and Azide free (AB324840)

This data was developed using ab322410, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : Mouse liver.

This antibody recognized mouse Tau (phospho T58), corresponding to human Tau (phospho T69).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Tau (phospho T69) antibody [EPR29149-92] (<a href='/en-us/products/primary-antibodies/tau-phospho-t69-antibody-epr29149-92-ab322410'>ab322410</a>) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate (untreated membrane) at 20 µg

Lane 2:

Mouse hippocampus tissue lysate (untreated membrane) at 20 µg

Lane 3:

Mouse liver tissue lysate (untreated membrane) at 20 µg

Lane 4:

Mouse brain tissue lysate (alkaline phosphatase treated membrane) at 20 µg

Lane 5:

Mouse hippocampus tissue lysate (alkaline phosphatase treated membrane) at 20 µg

Lane 6:

Mouse liver tissue lysate (alkaline phosphatase treated membrane) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 50-70 kDa,36 kDa

false

Exposure time: 180s

  • Unconjugated

    Anti-Tau (phospho T69) antibody [EPR29149-92]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR29149-92

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse

Applications

IHC-P, I-ELISA, WB, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This product do not work in cell lines, and is unsuitable for mouse IHC.
This antibody recognized mouse Tau (phospho T58), corresponding to human Tau (phospho T69).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Synthetic peptide": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p></p>", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell lysate - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

ab324840 is the carrier-free version of ab322410.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed : 21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed : 21985311, PubMed : 32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
See full target information MAPT pT69

Additional targets

Mapt
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com