Rabbit Polyclonal TAZ antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 13 publications.
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- Molecular reproduction and development 90:406-4162023Hsa_circ_0001740 mediates trophoblast cell function via regulating miR-188-3p/ARRDC3.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMei Long,Shan WangPubMed 37436094
- Nature communications 13:58052022Tubular cell polyploidy protects from lethal acute kidney injury but promotes consequent chronic kidney disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLetizia De Chiara,Carolina Conte,Roberto Semeraro,Paula Diaz-Bulnes,Maria Lucia Angelotti,Benedetta Mazzinghi,Alice Molli,Giulia Antonelli,Samuela Landini,Maria Elena Melica,Anna Julie Peired,Laura Maggi,Marta Donati,Gilda La Regina,Marco Allinovi,Fiammetta Ravaglia,Daniele Guasti,Daniele Bani,Luigi Cirillo,Francesca Becherucci,Francesco Guzzi,Alberto Magi,Francesco Annunziato,Laura Lasagni,Hans-Joachim Anders,Elena Lazzeri,Paola RomagnaniPubMed 36195583
- Evidence-based complementary and alternative medicine : eCAM 2022:62456472022FSCN1 Promotes Glycolysis and Epithelial-Mesenchymal Transition in Prostate Cancer through a YAP/TAZ Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMinghui Li,Zhiming Gao,Honglin Ding,Zhanhua Wang,Hada Mu,Lei Zhang,Jiufu Wei,Zhanshu MaPubMed 35815268
- American journal of physiology. Renal physiology 322:F419-F4282022TAZ is important for maintenance of the integrity of podocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianchun Chen,Xiaoyong Wang,Qian He,Raymond C HarrisPubMed 35157550
- Journal of the American Society of Nephrology : JA 31:996-10082020ARHGEF7 (-PIX) Is Required for the Maintenance of Podocyte Architecture and Glomerular Function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun Matsuda et. al.PubMed 32188698
- Molecular cancer therapeutics 19:247-2572019Modulating TAK1 Expression Inhibits YAP and TAZ Oncogenic Functions in Pancreatic Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRaffaela Santoro,Marco Zanotto,Francesca Simionato,Camilla Zecchetto,Valeria Merz,Chiara Cavallini,Geny Piro,Fabio Sabbadini,Federico Boschi,Aldo Scarpa,Davide MelisiPubMed 31562256
- Cell reports 27:1712-1725.e62019Linking YAP to Müller Glia Quiescence Exit in the Degenerative Retina.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnnaïg Hamon,Diana García-García,Divya Ail,Juliette Bitard,Albert Chesneau,Deniz Dalkara,Morgane Locker,Jérôme E Roger,Muriel PerronPubMed 31067458
- OncoTargets and therapy 12:3481-34892019miR-125a-5p inhibits colorectal cancer cell epithelial-mesenchymal transition, invasion and migration by targeting TAZ.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLei Tang et. al.PubMed 31190857
- Methods in molecular biology (Clifton, N.J.) 1893:107-1132018Immunofluorescence Microscopy to Study Endogenous TAZ in Mammalian Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNathan M Kingston,Andrew M Tilston-Lunel,Julia Hicks-Berthet,Xaralabos VarelasPubMed 30565129
- Molecular medicine reports 18:1674-16812018miR‑205 targets YAP1 and inhibits proliferation and invasion in thyroid cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDewei Li,Qiang Wang,Ning Li,Shuilong ZhangPubMed 29845281
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted |
| Chinese hamster | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Dog | Predicted | Predicted |
| Gorilla | Predicted | Predicted |
| Horse | Predicted | Predicted |
| Macaque monkey | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
| Pig | Predicted | Predicted |
| Rabbit | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Transcriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis (PubMed:11118213, PubMed:18227151). The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ (PubMed:18227151). WWTR1 enhances PAX8 and NKX2-1/TTF1-dependent gene activation (PubMed:19010321). In conjunction with YAP1, involved in the regulation of TGFB1-dependent SMAD2 and SMAD3 nuclear accumulation (PubMed:18568018). Plays a key role in coupling SMADs to the transcriptional machinery such as the mediator complex (PubMed:18568018). Regulates embryonic stem-cell self-renewal, promotes cell proliferation and epithelial-mesenchymal transition (PubMed:18227151, PubMed:18568018).
Alternative names
WW domain-containing transcription regulator protein 1, Transcriptional coactivator with PDZ-binding motif, WWTR1, TAZ
Recommended products
Rabbit Polyclonal TAZ antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 13 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAZ antibody (ab110239)
IHC image of TAZ staining in Human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab110239, 1μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Western blot - Anti-TAZ antibody (ab110239)
False colour image of Western blot: Anti-TAZ antibody staining at 1 ug/ml, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab110239 was shown to bind specifically to TAZ. A band was observed at 52 kDa in wild-type HeLa cell lysates with no signal observed at this size in WWTR1 knockout cell line Human WWTR1 knockout HeLa cell line ab281598 (knockout cell lysate ab282950). To generate this image, wild-type and WWTR1 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.
All lanes: Western blot - Anti-TAZ antibody (ab110239) at 1 µg/mL
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: WWTR1 knockout HeLa cell lysate at 20 µg
Lane 3: A549 cell lysate at 20 µg
Lane 4: K562 cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 44 kDa
Observed band size: 52 kDa
Western blot - Anti-TAZ antibody (ab110239)
All lanes: Western blot - Anti-TAZ antibody (ab110239) at 1 µg/mL
Lanes 1 and 3: A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate at 20 µg
Lanes 2 and 4: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 44 kDa
Observed band size: 36 kDa, 41 kDa, 53 kDa
Exposure time: 4min
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