Rabbit Polyclonal TAZ antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 22 publications. Immunogen corresponding to Recombinant Fragment Protein within Human WWTR1 aa 1-200.
Images
Publications
Filter by
- PloS one 18:e02911922023Selenium inhibits ferroptosis in hyperglycemic cerebral ischemia/reperfusion injury by stimulating the Hippo pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLu Li,Meng Wang,Yan-Mei Ma,Lan Yang,Deng-Hai Zhang,Feng-Ying Guo,Li Jing,Jian-Zhong ZhangPubMed 37682882
- Immunity, inflammation and disease 11:e9072023YES-associated protein-regulated Smad7 worsen epithelial barrier injury of chronic sinusitis with nasal polyps.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaocong Jiang,Longlan Shu,Yijun Liu,Yang Shen,Xia Ke,Jie Liu,Yucheng YangPubMed 37382248
- iScience 26:1070252023Resistin targets TAZ to promote osteogenic differentiation through PI3K/AKT/mTOR pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJingJing Shang,Zhentang Yu,Chengwei Xiong,Junjie Zhang,Jinhong Gong,Changlin Yu,Yong Huang,Xindie ZhouPubMed 37389179
- iScience 26:1068622023macrophages promote hepatocellular carcinoma tumor stemness and progression through /β-catenin/ axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHai-Chao Zhao,Chang-Zhou Chen,Yan-Zhang Tian,Huang-Qin Song,Xiao-Xiao Wang,Yan-Jun Li,Jie-Feng He,Hao-Liang ZhaoPubMed 37275516
- Cell death & disease 14:2642023USP1 modulates hepatocellular carcinoma progression via the Hippo/TAZ axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDongyi Liu,Quanhui Li,Yifeng Zang,Xin Li,Zhongbo Li,Peng Zhang,Chang Feng,Penghe Yang,Jiayao Cui,Yanan Sun,Tian Wei,Peng Su,Xin Zhao,Huijie Yang,Yinlu DingPubMed 37041150
- The Journal of clinical investigation 132:2022SCF-SKP2 E3 ubiquitin ligase links mTORC1/ER stress/ISR with YAP activation in murine renal cystogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDibyendu K Panda,Xiuying Bai,Yan Zhang,Nicholas A Stylianesis,Antonis E Koromilas,Mark L Lipman,Andrew C KaraplisPubMed 36326820
- Frontiers in pharmacology 13:7978732022Cinobufacini Injection Inhibits the Proliferation of Triple-Negative Breast Cancer Through the Pin1-TAZ Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLu Kong,Xu Liu,Bing Yu,Ye Yuan,Qianru Zhao,Yuru Chen,Bin Qu,Xue Du,Xiaoxuan Tian,Rui Shao,Yu WangPubMed 35450041
- Frontiers in oncology 12:8396032022α-Hederin Inhibits the Proliferation of Hepatocellular Carcinoma Cells Hippo-Yes-Associated Protein Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTongqing Chen,Dongdong Sun,Qijuan Wang,Tingting Zhou,Jiani Tan,Changliang Xu,Haibo Cheng,Weixing ShenPubMed 35311132
- Stem cells international 2021:66443662021CGF Membrane Promotes Periodontal Tissue Regeneration Mediated by hUCMSCs through Upregulating TAZ and Osteogenic Differentiation Genes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenjing Li,Feifei Wang,Fusheng Dong,Zhiyong Zhang,Peng Song,Huizhen Chen,Jie WangPubMed 34394357
- Discover. Oncology 12:122021HOXC10 promotes growth and migration of melanoma by regulating Slug to activate the YAP/TAZ signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuanxin Miao,Weina Zhang,Su Liu,Xiangfeng Leng,Chunnan Hu,Hao SunPubMed 35201457
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human WWTR1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q9GZV5
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Transcriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis (PubMed:11118213, PubMed:18227151, PubMed:23911299). The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ (PubMed:18227151). WWTR1 enhances PAX8 and NKX2-1/TTF1-dependent gene activation (PubMed:19010321). In conjunction with YAP1, involved in the regulation of TGFB1-dependent SMAD2 and SMAD3 nuclear accumulation (PubMed:18568018). Plays a key role in coupling SMADs to the transcriptional machinery such as the mediator complex (PubMed:18568018). Regulates embryonic stem-cell self-renewal, promotes cell proliferation and epithelial-mesenchymal transition (PubMed:18227151, PubMed:18568018).
Alternative names
TAZ, WWTR1, WW domain-containing transcription regulator protein 1, Transcriptional coactivator with PDZ-binding motif
Recommended products
Rabbit Polyclonal TAZ antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 22 publications. Immunogen corresponding to Recombinant Fragment Protein within Human WWTR1 aa 1-200.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human WWTR1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q9GZV5
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TAZ also known as WWTR1 (WW Domain-Containing Transcription Regulator 1) acts as a transcriptional co-activator in cells. TAZ has a molecular mass of approximately 45 kDa. This protein appears in various tissues including the lung kidney brain and heart. TAZ interacts with other transcription factors to influence gene expression playing a role in cellular signaling and development.
- Biological function summary
TAZ works as a part of the Hippo signaling pathway regulating cell growth proliferation and apoptosis. It changes cellular responses through interactions with other proteins such as TEAD transcription factors. TAZ can act within the cytoplasm or the nucleus changing its location in the cell based on physiological signals. It operates independently as well as in concert with other molecules assisting in maintaining tissue homeostasis and regeneration.
- Pathways
TAZ functions as an essential element of the Hippo pathway which controls organ size and suppresses cancer. It plays significant roles in the PI3K-AKT signaling pathway coordinating with YAP (Yes-associated protein) a well-known partner in these pathways. TAZ and YAP together influence transcriptional outcomes for numerous genes adjusting to the needs of various cellular contexts which affect cell behavior significantly.
- Associated diseases and disorders
TAZ associates with cancer and fibrosis. In many cancers TAZ exhibits high activity often linked with poor prognosis due to its role in promoting cell proliferation and survival. TAZ interacts with beta-catenin in cancer contexts influencing signaling that drives cancer progression. In fibrosis aberrant TAZ signaling leads to excessive tissue scarring working alongside CTGF (Connective Tissue Growth Factor) to support this pathological development.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
Western blot - Anti-TAZ antibody (ab224239)
All lanes: Western blot - Anti-TAZ antibody (ab224239) at 1/250 dilution
All lanes: Human kidney tissue lysate
Developed using the ECL technique.
Predicted band size: 44 kDa
Immunocytochemistry/ Immunofluorescence - Anti-TAZ antibody (ab224239)
PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for TAZ (green) using ab224239 at 4 μg/ml in ICC/IF.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAZ antibody (ab224239)
Paraffin-embedded human placenta tissue stained for TAZ using ab224239 at 1/50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAZ antibody (ab224239)
Paraffin-embedded human kidney tissue stained for TAZ using ab224239 at 1/50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAZ antibody (ab224239)
Paraffin-embedded human renal cancer tissue stained for TAZ using ab224239 at 1/50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAZ antibody (ab224239)
Paraffin-embedded human colon tissue stained for TAZ using ab224239 at 1/50 dilution in immunohistochemical analysis.
Western blot - Anti-TAZ antibody (ab224239)
All lanes: Western blot - Anti-TAZ antibody (ab224239)
Lane 1: EFO-21 cells transfected with control siRNA
Lane 2: EFO-21 cells transfected with target specific siRNA probe 1
Lane 3: EFO-21 cells transfected with target specific siRNA probe 2
Predicted band size: 44 kDa
Western blot - Anti-TAZ antibody (ab224239)
All lanes: Western blot - Anti-TAZ antibody (ab224239) at 1/250 dilution
All lanes: NIH/3T3 (mouse embryonic fibroblast cell line) cell lysate
Predicted band size: 44 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com