AB224239

Anti-TAZ antibody

Name: Anti-TAZ antibody (ab224239) | Abcam
Brand: Abcam Limited
SKU: AB224239
Price: 535 USD
Availability: InStock
Rating: 1 (1 reviews)

(1 Review)

(29 Publications)

Rabbit Polyclonal TAZ antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 29 publications. Immunogen corresponding to Recombinant Fragment Protein within Human WWTR1 aa 1-200.

View Alternative Names

TAZ, WWTR1, WW domain-containing transcription regulator protein 1, Transcriptional coactivator with PDZ-binding motif

8 Images

Immunocytochemistry/ Immunofluorescence - Anti-TAZ antibody (AB224239)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TAZ antibody (AB224239)

PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for TAZ (green) using ab224239 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAZ antibody (AB224239)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAZ antibody (AB224239)

Paraffin-embedded human placenta tissue stained for TAZ using ab224239 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAZ antibody (AB224239)

Paraffin-embedded human colon tissue stained for TAZ using ab224239 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAZ antibody (AB224239)

Paraffin-embedded human kidney tissue stained for TAZ using ab224239 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAZ antibody (AB224239)

Paraffin-embedded human renal cancer tissue stained for TAZ using ab224239 at 1/50 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-TAZ antibody (AB224239)

All lanes:

Western blot - Anti-TAZ antibody (ab224239) at 1/250 dilution

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Human kidney tissue lysate

Predicted band size: 44 kDa

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Supplier Data

Western blot - Anti-TAZ antibody (AB224239)

All lanes:

Western blot - Anti-TAZ antibody (ab224239)

Lane 1:

EFO-21 cells transfected with control siRNA

Lane 2:

EFO-21 cells transfected with target specific siRNA probe 1

Lane 3:

EFO-21 cells transfected with target specific siRNA probe 2

Predicted band size: 44 kDa

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Supplier Data

Western blot - Anti-TAZ antibody (AB224239)

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Western blot - Anti-TAZ antibody (ab224239) at 1/250 dilution

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NIH/3T3 (mouse embryonic fibroblast cell line) cell lysate

Predicted band size: 44 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human WWTR1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q9GZV5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TAZ also known as WWTR1 (WW Domain-Containing Transcription Regulator 1) acts as a transcriptional co-activator in cells. TAZ has a molecular mass of approximately 45 kDa. This protein appears in various tissues including the lung kidney brain and heart. TAZ interacts with other transcription factors to influence gene expression playing a role in cellular signaling and development.

Biological function summary

TAZ works as a part of the Hippo signaling pathway regulating cell growth proliferation and apoptosis. It changes cellular responses through interactions with other proteins such as TEAD transcription factors. TAZ can act within the cytoplasm or the nucleus changing its location in the cell based on physiological signals. It operates independently as well as in concert with other molecules assisting in maintaining tissue homeostasis and regeneration.

Pathways

TAZ functions as an essential element of the Hippo pathway which controls organ size and suppresses cancer. It plays significant roles in the PI3K-AKT signaling pathway coordinating with YAP (Yes-associated protein) a well-known partner in these pathways. TAZ and YAP together influence transcriptional outcomes for numerous genes adjusting to the needs of various cellular contexts which affect cell behavior significantly.

TAZ associates with cancer and fibrosis. In many cancers TAZ exhibits high activity often linked with poor prognosis due to its role in promoting cell proliferation and survival. TAZ interacts with beta-catenin in cancer contexts influencing signaling that drives cancer progression. In fibrosis aberrant TAZ signaling leads to excessive tissue scarring working alongside CTGF (Connective Tissue Growth Factor) to support this pathological development.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis (PubMed : 11118213, PubMed : 18227151, PubMed : 23911299). The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ (PubMed : 18227151). WWTR1 enhances PAX8 and NKX2-1/TTF1-dependent gene activation (PubMed : 19010321). In conjunction with YAP1, involved in the regulation of TGFB1-dependent SMAD2 and SMAD3 nuclear accumulation (PubMed : 18568018). Plays a key role in coupling SMADs to the transcriptional machinery such as the mediator complex (PubMed : 18568018). Regulates embryonic stem-cell self-renewal, promotes cell proliferation and epithelial-mesenchymal transition (PubMed : 18227151, PubMed : 18568018).

See full target information WWTR1

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Publications (29)

Recent publications for all applications. Explore the full list and refine your search

Journal of experimental & clinical cancer research : CR 44:247 PubMed40842027

2025

Proteomic profiling identifies a stromal TGF-β1/podoplanin axis as a driver of colorectal cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Silvia Di Agostino,Davide La Padula,Vittoria Rago,Caterina Gabriele,Francesco Conforti,Elio Aprigliano,Lidia Urlandini,Elvira Parrotta,Danilo Lofaro,Francesca Vescio,Andrea Sacconi,Valeria Cernaro,Giuseppe Currò,Angela Alibrandi,Girolamo Ranieri,Valeria Zuccalà,Antonio Ieni,Marco Gaspari,Giovanni Cuda,Michele Ammendola,Vittorio Abbonante

Respiratory research 26:51 PubMed39939959

2025

YAP as a potential therapeutic target for myofibroblast formation in asthma.

Applications

Unspecified application

Species

Unspecified reactive species

Yanrong Guo,Yuran Zhou,Rui Wang,Yujing Lin,Huimin Lan,Yang Li,De-Yun Wang,Jinrui Dong,Kefeng Li,Yan Yan,Yongkang Qiao

Cell biology and toxicology 41:9 PubMed39707043

2024

M6A modification-mediated LIMA1 promotes the progression of hepatocellular carcinoma through the wnt-βcatenin/Hippo pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Zhang,Xiaoxiao Wang,Huangqin Song,Junlong Yuan,Xiaomin Zhang,Yiran Yuan,Zhuangqiang Wang,Zhang Lei,Jiefeng He

Nature communications 15:7885 PubMed39251588

2024

Defective N-glycosylation of IL6 induces metastasis and tyrosine kinase inhibitor resistance in lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Chun-Hua Hung,Shang-Yin Wu,Cheng-I Daniel Yao,Hsuan-Heng Yeh,Chien-Chung Lin,Chang-Yao Chu,Tzu-Yu Huang,Meng-Ru Shen,Chun-Hung Lin,Wu-Chou Su

PloS one 18:e0291192 PubMed37682882

2023

Selenium inhibits ferroptosis in hyperglycemic cerebral ischemia/reperfusion injury by stimulating the Hippo pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Li,Meng Wang,Yan-Mei Ma,Lan Yang,Deng-Hai Zhang,Feng-Ying Guo,Li Jing,Jian-Zhong Zhang

Immunity, inflammation and disease 11:e907 PubMed37382248

2023

YES-associated protein-regulated Smad7 worsen epithelial barrier injury of chronic sinusitis with nasal polyps.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaocong Jiang,Longlan Shu,Yijun Liu,Yang Shen,Xia Ke,Jie Liu,Yucheng Yang

iScience 26:107025 PubMed37389179

2023

Resistin targets TAZ to promote osteogenic differentiation through PI3K/AKT/mTOR pathway.

Applications

Unspecified application

Species

Unspecified reactive species

JingJing Shang,Zhentang Yu,Chengwei Xiong,Junjie Zhang,Jinhong Gong,Changlin Yu,Yong Huang,Xindie Zhou

iScience 26:106862 PubMed37275516

2023

macrophages promote hepatocellular carcinoma tumor stemness and progression through /β-catenin/ axis.

Applications

Unspecified application

Species

Unspecified reactive species

Hai-Chao Zhao,Chang-Zhou Chen,Yan-Zhang Tian,Huang-Qin Song,Xiao-Xiao Wang,Yan-Jun Li,Jie-Feng He,Hao-Liang Zhao

Cell death & disease 14:264 PubMed37041150

2023

USP1 modulates hepatocellular carcinoma progression via the Hippo/TAZ axis.

Applications

Unspecified application

Species

Unspecified reactive species

Dongyi Liu,Quanhui Li,Yifeng Zang,Xin Li,Zhongbo Li,Peng Zhang,Chang Feng,Penghe Yang,Jiayao Cui,Yanan Sun,Tian Wei,Peng Su,Xin Zhao,Huijie Yang,Yinlu Ding

The Journal of clinical investigation 132: PubMed36326820

2022

SCF-SKP2 E3 ubiquitin ligase links mTORC1/ER stress/ISR with YAP activation in murine renal cystogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Dibyendu K Panda,Xiuying Bai,Yan Zhang,Nicholas A Stylianesis,Antonis E Koromilas,Mark L Lipman,Andrew C Karaplis

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TAZ antibody

Immunocytochemistry/ Immunofluorescence - Anti-TAZ antibody (AB224239)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAZ antibody (AB224239)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAZ antibody (AB224239)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAZ antibody (AB224239)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAZ antibody (AB224239)

Western blot - Anti-TAZ antibody (AB224239)

Western blot - Anti-TAZ antibody (AB224239)

Western blot - Anti-TAZ antibody (AB224239)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (29)

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