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Mouse Monoclonal TAZ antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Recombinant Fragment Protein within Human WWTR1 aa 1-200.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAZ antibody [CL0371] (AB242313), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-TAZ antibody [CL0371] (AB242313), expandable thumbnail
  • Western blot - Anti-TAZ antibody [CL0371] (AB242313), expandable thumbnail
  • Western blot - Anti-TAZ antibody [CL0371] (AB242313), expandable thumbnail
  • Western blot - Anti-TAZ antibody [CL0371] (AB242313), expandable thumbnail

Publications

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human WWTR1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q9GZV5

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Tested
Tested
Tested
Mouse
Predicted
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1/50.00000 - 1/200.00000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species

Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1 µg/mL

Notes

-

Predicted
Predicted

Species

Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

2.00000-10.00000 µg/mL

Notes

Fixation/Permeabilization: PFA/Triton X-100.

Predicted
Predicted

Species

Mouse

Dilution info

-

Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Transcriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis (PubMed:11118213, PubMed:18227151, PubMed:23911299). The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ (PubMed:18227151). WWTR1 enhances PAX8 and NKX2-1/TTF1-dependent gene activation (PubMed:19010321). In conjunction with YAP1, involved in the regulation of TGFB1-dependent SMAD2 and SMAD3 nuclear accumulation (PubMed:18568018). Plays a key role in coupling SMADs to the transcriptional machinery such as the mediator complex (PubMed:18568018). Regulates embryonic stem-cell self-renewal, promotes cell proliferation and epithelial-mesenchymal transition (PubMed:18227151, PubMed:18568018).

Alternative names

Recommended products

Mouse Monoclonal TAZ antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Recombinant Fragment Protein within Human WWTR1 aa 1-200.

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Recombinant Fragment Protein within Human WWTR1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q9GZV5
Clone number

CL0371

Purification technique

Affinity purification Protein A

Concentration
Loading...
Purification notes

Purified from TCS.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

TAZ also known as WWTR1 (WW Domain-Containing Transcription Regulator 1) acts as a transcriptional co-activator in cells. TAZ has a molecular mass of approximately 45 kDa. This protein appears in various tissues including the lung kidney brain and heart. TAZ interacts with other transcription factors to influence gene expression playing a role in cellular signaling and development.

Biological function summary

TAZ works as a part of the Hippo signaling pathway regulating cell growth proliferation and apoptosis. It changes cellular responses through interactions with other proteins such as TEAD transcription factors. TAZ can act within the cytoplasm or the nucleus changing its location in the cell based on physiological signals. It operates independently as well as in concert with other molecules assisting in maintaining tissue homeostasis and regeneration.

Pathways

TAZ functions as an essential element of the Hippo pathway which controls organ size and suppresses cancer. It plays significant roles in the PI3K-AKT signaling pathway coordinating with YAP (Yes-associated protein) a well-known partner in these pathways. TAZ and YAP together influence transcriptional outcomes for numerous genes adjusting to the needs of various cellular contexts which affect cell behavior significantly.

Associated diseases and disorders

TAZ associates with cancer and fibrosis. In many cancers TAZ exhibits high activity often linked with poor prognosis due to its role in promoting cell proliferation and survival. TAZ interacts with beta-catenin in cancer contexts influencing signaling that drives cancer progression. In fibrosis aberrant TAZ signaling leads to excessive tissue scarring working alongside CTGF (Connective Tissue Growth Factor) to support this pathological development.

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8 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAZ antibody [CL0371] (ab242313), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAZ antibody [CL0371] (ab242313)

    Formalin-fixed, paraffin-embedded human breast cancer tissue stained for TAZ with ab242313 at a 1:50 dilution in immunohistochemical analysis.

  • Immunocytochemistry/ Immunofluorescence - Anti-TAZ antibody [CL0371] (ab242313), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-TAZ antibody [CL0371] (ab242313)

    Immunocytochemistry/Immunofluorescence analysis of SK-MEL cells labeling TAZ using ab242313 at 4 μg/ml (green). Microtubules are visualized in red.

  • Western blot - Anti-TAZ antibody [CL0371] (ab242313), expandable thumbnail

    Western blot - Anti-TAZ antibody [CL0371] (ab242313)

    False colour image of Western blot: Anti-TAZ antibody [CL0371] staining at 1 ug/ml, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab242313 was shown to bind specifically to TAZ. A band was observed at 52 kDa in wild-type HeLa cell lysates with no signal observed at this size in WWTR1 knockout cell line Human WWTR1 knockout HeLa cell line ab281598 (knockout cell lysate ab282950). To generate this image, wild-type and WWTR1 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) at 1/20000 dilution.

    All lanes: Western blot - Anti-TAZ antibody [CL0371] (ab242313) at 1 µg/mL

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: WWTR1 knockout HeLa cell lysate at 20 µg

    Lane 3: A549 cell lysate at 20 µg

    Lane 4: K562 cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 44 kDa

    Observed band size: 52 kDa

  • Western blot - Anti-TAZ antibody [CL0371] (ab242313), expandable thumbnail

    Western blot - Anti-TAZ antibody [CL0371] (ab242313)

    Loading control: anti-PPIB.

    All lanes: Western blot - Anti-TAZ antibody [CL0371] (ab242313) at 1 µg/mL

    Lane 1: Control siRNA transfected EFO-21 cell lysate

    Lane 2: siRNA#1 transfected EFO-21 cell lysate

    Lane 3: siRNA#2 transfected EFO-21 cell lysate

    Predicted band size: 44 kDa

  • Western blot - Anti-TAZ antibody [CL0371] (ab242313), expandable thumbnail

    Western blot - Anti-TAZ antibody [CL0371] (ab242313)

    All lanes: Western blot - Anti-TAZ antibody [CL0371] (ab242313) at 1 µg/mL

    All lanes: U-251 MG (Human brain glioma cell line) cell lysate

    Predicted band size: 44 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAZ antibody [CL0371] (ab242313), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAZ antibody [CL0371] (ab242313)

    Negative control:

    Formalin-fixed, paraffin-embedded human lymph node tissue stained for TAZ with ab242313 at a 1:50 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAZ antibody [CL0371] (ab242313), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAZ antibody [CL0371] (ab242313)

    Formalin-fixed, paraffin-embedded human kidney tissue stained for TAZ with ab242313 at a 1:50 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAZ antibody [CL0371] (ab242313), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAZ antibody [CL0371] (ab242313)

    Formalin-fixed, paraffin-embedded human placenta tissue stained for TAZ with ab242313 at a 1:50 dilution in immunohistochemical analysis.

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