Anti-TAZ antibody [CL0371]

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TAZ antibody [CL0371] (AB242313)

Immunocytochemistry/Immunofluorescence analysis of SK-MEL cells labeling TAZ using ab242313 at 4 μg/ml (green). Microtubules are visualized in red.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAZ antibody [CL0371] (AB242313)

Formalin-fixed, paraffin-embedded human breast cancer tissue stained for TAZ with ab242313 at a 1 : 50 dilution in immunohistochemical analysis.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAZ antibody [CL0371] (AB242313)

Formalin-fixed, paraffin-embedded human placenta tissue stained for TAZ with ab242313 at a 1 : 50 dilution in immunohistochemical analysis.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAZ antibody [CL0371] (AB242313)

Formalin-fixed, paraffin-embedded human kidney tissue stained for TAZ with ab242313 at a 1 : 50 dilution in immunohistochemical analysis.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAZ antibody [CL0371] (AB242313)

Negative control :

Formalin-fixed, paraffin-embedded human lymph node tissue stained for TAZ with ab242313 at a 1 : 50 dilution in immunohistochemical analysis.

• WB

Lab

Western blot - Anti-TAZ antibody [CL0371] (AB242313)

False colour image of Western blot : Anti-TAZ antibody [CL0371] staining at 1 ug/ml, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (ab181602) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab242313 was shown to bind specifically to TAZ. A band was observed at 52 kDa in wild-type HeLa cell lysates with no signal observed at this size in WWTR1 knockout cell line ab281598 (knockout cell lysate ab282950). To generate this image, wild-type and WWTR1 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye^® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye^® 680RD) preabsorbed (ab216777) at 1/20000 dilution.

All lanes:

Western blot - Anti-TAZ antibody [CL0371] (ab242313) at 1 µg/mL

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

WWTR1 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human WWTR1 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-wwtr1-knockout-hela-cell-line-ab281598'>ab281598</a>)

Lane 3:

A549 cell lysate at 20 µg

Lane 4:

K562 cell lysate at 20 µg

Predicted band size: 44 kDa

Observed band size: 52 kDa

false

• WB

Supplier Data

Western blot - Anti-TAZ antibody [CL0371] (AB242313)

Loading control : anti-PPIB.

All lanes:

Western blot - Anti-TAZ antibody [CL0371] (ab242313) at 1 µg/mL

Lane 1:

Control siRNA transfected EFO-21 cell lysate

Lane 2:

siRNA#1 transfected EFO-21 cell lysate

Lane 3:

siRNA#2 transfected EFO-21 cell lysate

Predicted band size: 44 kDa

false

• WB

Supplier Data

Western blot - Anti-TAZ antibody [CL0371] (AB242313)

All lanes:

Western blot - Anti-TAZ antibody [CL0371] (ab242313) at 1 µg/mL

All lanes:

U-251 MG (Human brain glioma cell line) cell lysate

Predicted band size: 44 kDa

false