Mouse Monoclonal TAZ antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Recombinant Fragment Protein within Human WWTR1 aa 1-200.
IgG1
Mouse
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Liquid
Monoclonal
IHC-P | WB | ICC/IF | |
---|---|---|---|
Human | Tested | Tested | Tested |
Mouse | Predicted | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 2.00000-10.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes - |
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Transcriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis (PubMed:11118213, PubMed:18227151, PubMed:23911299). The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ (PubMed:18227151). WWTR1 enhances PAX8 and NKX2-1/TTF1-dependent gene activation (PubMed:19010321). In conjunction with YAP1, involved in the regulation of TGFB1-dependent SMAD2 and SMAD3 nuclear accumulation (PubMed:18568018). Plays a key role in coupling SMADs to the transcriptional machinery such as the mediator complex (PubMed:18568018). Regulates embryonic stem-cell self-renewal, promotes cell proliferation and epithelial-mesenchymal transition (PubMed:18227151, PubMed:18568018).
TAZ, WWTR1, WW domain-containing transcription regulator protein 1, Transcriptional coactivator with PDZ-binding motif
Mouse Monoclonal TAZ antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Recombinant Fragment Protein within Human WWTR1 aa 1-200.
IgG1
Mouse
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Liquid
Monoclonal
CL0371
Affinity purification Protein A
Purified from TCS.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
TAZ also known as WWTR1 (WW Domain-Containing Transcription Regulator 1) acts as a transcriptional co-activator in cells. TAZ has a molecular mass of approximately 45 kDa. This protein appears in various tissues including the lung kidney brain and heart. TAZ interacts with other transcription factors to influence gene expression playing a role in cellular signaling and development.
TAZ works as a part of the Hippo signaling pathway regulating cell growth proliferation and apoptosis. It changes cellular responses through interactions with other proteins such as TEAD transcription factors. TAZ can act within the cytoplasm or the nucleus changing its location in the cell based on physiological signals. It operates independently as well as in concert with other molecules assisting in maintaining tissue homeostasis and regeneration.
TAZ functions as an essential element of the Hippo pathway which controls organ size and suppresses cancer. It plays significant roles in the PI3K-AKT signaling pathway coordinating with YAP (Yes-associated protein) a well-known partner in these pathways. TAZ and YAP together influence transcriptional outcomes for numerous genes adjusting to the needs of various cellular contexts which affect cell behavior significantly.
TAZ associates with cancer and fibrosis. In many cancers TAZ exhibits high activity often linked with poor prognosis due to its role in promoting cell proliferation and survival. TAZ interacts with beta-catenin in cancer contexts influencing signaling that drives cancer progression. In fibrosis aberrant TAZ signaling leads to excessive tissue scarring working alongside CTGF (Connective Tissue Growth Factor) to support this pathological development.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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Formalin-fixed, paraffin-embedded human breast cancer tissue stained for TAZ with ab242313 at a 1:50 dilution in immunohistochemical analysis.
Immunocytochemistry/Immunofluorescence analysis of SK-MEL cells labeling TAZ using ab242313 at 4 μg/ml (green). Microtubules are visualized in red.
False colour image of Western blot: Anti-TAZ antibody [CL0371] staining at 1 ug/ml, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab242313 was shown to bind specifically to TAZ. A band was observed at 52 kDa in wild-type HeLa cell lysates with no signal observed at this size in WWTR1 knockout cell line Human WWTR1 knockout HeLa cell line ab281598 (knockout cell lysate ab282950). To generate this image, wild-type and WWTR1 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) at 1/20000 dilution.
All lanes: Western blot - Anti-TAZ antibody [CL0371] (ab242313) at 1 µg/mL
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: WWTR1 knockout HeLa cell lysate at 20 µg
Lane 3: A549 cell lysate at 20 µg
Lane 4: K562 cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 44 kDa
Observed band size: 52 kDa
Loading control: anti-PPIB.
All lanes: Western blot - Anti-TAZ antibody [CL0371] (ab242313) at 1 µg/mL
Lane 1: Control siRNA transfected EFO-21 cell lysate
Lane 2: siRNA#1 transfected EFO-21 cell lysate
Lane 3: siRNA#2 transfected EFO-21 cell lysate
Predicted band size: 44 kDa
All lanes: Western blot - Anti-TAZ antibody [CL0371] (ab242313) at 1 µg/mL
All lanes: U-251 MG (Human brain glioma cell line) cell lysate
Predicted band size: 44 kDa
Negative control:
Formalin-fixed, paraffin-embedded human lymph node tissue stained for TAZ with ab242313 at a 1:50 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human kidney tissue stained for TAZ with ab242313 at a 1:50 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human placenta tissue stained for TAZ with ab242313 at a 1:50 dilution in immunohistochemical analysis.
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