Skip to main content

Rabbit Recombinant Multiclonal TAZ phospho S311 antibody. Suitable for ICC and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human WWTR1 phospho S311 aa 300-350.

Be the first to review this product! Submit a review

Images

Immunocytochemistry - Anti-TAZ (phospho S311) antibody [14HCLC] (AB277791), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: 99.91% PBS

Form
Liquid
Clonality
Multiclonal

Immunogen

  • Synthetic Peptide within Human WWTR1 phospho S311 aa 300-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q9GZV5

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC
Human
Tested

Tested
Tested

Species
Human
Dilution info
1/100
Notes

-

Target data

Function

Transcriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis (PubMed:11118213, PubMed:18227151, PubMed:23911299). The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ (PubMed:18227151). WWTR1 enhances PAX8 and NKX2-1/TTF1-dependent gene activation (PubMed:19010321). In conjunction with YAP1, involved in the regulation of TGFB1-dependent SMAD2 and SMAD3 nuclear accumulation (PubMed:18568018). Plays a key role in coupling SMADs to the transcriptional machinery such as the mediator complex (PubMed:18568018). Regulates embryonic stem-cell self-renewal, promotes cell proliferation and epithelial-mesenchymal transition (PubMed:18227151, PubMed:18568018).

Alternative names

TAZ, WWTR1, WW domain-containing transcription regulator protein 1, Transcriptional coactivator with PDZ-binding motif

Recommended products

Rabbit Recombinant Multiclonal TAZ phospho S311 antibody. Suitable for ICC and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human WWTR1 phospho S311 aa 300-350.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Multiclonal
Immunogen
  • Synthetic Peptide within Human WWTR1 phospho S311 aa 300-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q9GZV5
Clone number
14HCLC
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle

Notes

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

TAZ also known as WWTR1 (WW Domain-Containing Transcription Regulator 1) acts as a transcriptional co-activator in cells. TAZ has a molecular mass of approximately 45 kDa. This protein appears in various tissues including the lung kidney brain and heart. TAZ interacts with other transcription factors to influence gene expression playing a role in cellular signaling and development.

Biological function summary

TAZ works as a part of the Hippo signaling pathway regulating cell growth proliferation and apoptosis. It changes cellular responses through interactions with other proteins such as TEAD transcription factors. TAZ can act within the cytoplasm or the nucleus changing its location in the cell based on physiological signals. It operates independently as well as in concert with other molecules assisting in maintaining tissue homeostasis and regeneration.

Pathways

TAZ functions as an essential element of the Hippo pathway which controls organ size and suppresses cancer. It plays significant roles in the PI3K-AKT signaling pathway coordinating with YAP (Yes-associated protein) a well-known partner in these pathways. TAZ and YAP together influence transcriptional outcomes for numerous genes adjusting to the needs of various cellular contexts which affect cell behavior significantly.

Associated diseases and disorders

TAZ associates with cancer and fibrosis. In many cancers TAZ exhibits high activity often linked with poor prognosis due to its role in promoting cell proliferation and survival. TAZ interacts with beta-catenin in cancer contexts influencing signaling that drives cancer progression. In fibrosis aberrant TAZ signaling leads to excessive tissue scarring working alongside CTGF (Connective Tissue Growth Factor) to support this pathological development.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Immunocytochemistry - Anti-TAZ (phospho S311) antibody [14HCLC] (ab277791), expandable thumbnail

    Immunocytochemistry - Anti-TAZ (phospho S311) antibody [14HCLC] (ab277791)

    For immunofluorescence analysis, HeLa cells were fixed and permeabilized for detection of endogenous WWTR1 pS311 using Anti-Phospho-WWTR1 (Ser311) Recombinant Rabbit Multiclonal Antibody (ab277791, 1/100) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (1/2000). Nuclei (blue) were stained using ProLong Diamond Antifade Mountant with DAPI(Product # P36962) and cytoskeletal F-actin (red) staining using Rhodamine Phalloidin (1/300) Panel a-d) shows representative un-treated cells that were stained for detection and localization of Phospho-WWTR1 (Ser311) protein (green) with no signal compared to panel e-h) clearly demonstrating enhanced cytoplasmic localisation of WWTR1 pS311 in HeLa cells treated with Okadaic acid (1 M, 1h) and MG132 (40 M, 3h). The images were captured at 60X magnification.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com