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AB277791

Anti-TAZ (phospho S311) antibody [14HCLC]

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(3 Publications)

Rabbit Recombinant Multiclonal TAZ phospho S311 antibody. Suitable for ICC and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human WWTR1 phospho S311 aa 300-350.

View Alternative Names

TAZ, WWTR1, WW domain-containing transcription regulator protein 1, Transcriptional coactivator with PDZ-binding motif

1 Images
Immunocytochemistry - Anti-TAZ (phospho S311) antibody [14HCLC] (AB277791)
  • ICC

Supplier Data

Immunocytochemistry - Anti-TAZ (phospho S311) antibody [14HCLC] (AB277791)

For immunofluorescence analysis, HeLa cells were fixed and permeabilized for detection of endogenous WWTR1 pS311 using Anti-Phospho-WWTR1 (Ser311) Recombinant Rabbit Multiclonal Antibody (ab277791, 1/100) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (1/2000). Nuclei (blue) were stained using ProLong Diamond Antifade Mountant with DAPI(Product # P36962) and cytoskeletal F-actin (red) staining using Rhodamine Phalloidin (1/300) Panel a-d) shows representative un-treated cells that were stained for detection and localization of Phospho-WWTR1 (Ser311) protein (green) with no signal compared to panel e-h) clearly demonstrating enhanced cytoplasmic localisation of WWTR1 pS311 in HeLa cells treated with Okadaic acid (1 M, 1h) and MG132 (40 M, 3h). The images were captured at 60X magnification.

Key facts

Host species

Rabbit

Clonality

Multiclonal

Clone number

14HCLC

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC

applications

Immunogen

Synthetic Peptide within Human WWTR1 phospho S311 aa 300-350. The exact immunogen used to generate this antibody is proprietary information.

Q9GZV5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/100", "ICC-species-notes": "<p></p>" } } }
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Product details

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TAZ also known as WWTR1 (WW Domain-Containing Transcription Regulator 1) acts as a transcriptional co-activator in cells. TAZ has a molecular mass of approximately 45 kDa. This protein appears in various tissues including the lung kidney brain and heart. TAZ interacts with other transcription factors to influence gene expression playing a role in cellular signaling and development.
Biological function summary

TAZ works as a part of the Hippo signaling pathway regulating cell growth proliferation and apoptosis. It changes cellular responses through interactions with other proteins such as TEAD transcription factors. TAZ can act within the cytoplasm or the nucleus changing its location in the cell based on physiological signals. It operates independently as well as in concert with other molecules assisting in maintaining tissue homeostasis and regeneration.

Pathways

TAZ functions as an essential element of the Hippo pathway which controls organ size and suppresses cancer. It plays significant roles in the PI3K-AKT signaling pathway coordinating with YAP (Yes-associated protein) a well-known partner in these pathways. TAZ and YAP together influence transcriptional outcomes for numerous genes adjusting to the needs of various cellular contexts which affect cell behavior significantly.

TAZ associates with cancer and fibrosis. In many cancers TAZ exhibits high activity often linked with poor prognosis due to its role in promoting cell proliferation and survival. TAZ interacts with beta-catenin in cancer contexts influencing signaling that drives cancer progression. In fibrosis aberrant TAZ signaling leads to excessive tissue scarring working alongside CTGF (Connective Tissue Growth Factor) to support this pathological development.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis (PubMed : 11118213, PubMed : 18227151, PubMed : 23911299). The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ (PubMed : 18227151). WWTR1 enhances PAX8 and NKX2-1/TTF1-dependent gene activation (PubMed : 19010321). In conjunction with YAP1, involved in the regulation of TGFB1-dependent SMAD2 and SMAD3 nuclear accumulation (PubMed : 18568018). Plays a key role in coupling SMADs to the transcriptional machinery such as the mediator complex (PubMed : 18568018). Regulates embryonic stem-cell self-renewal, promotes cell proliferation and epithelial-mesenchymal transition (PubMed : 18227151, PubMed : 18568018).
See full target information WWTR1 phospho S311
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Discover oncology 15:73 PubMed38478152

2024

GALNT2 targeted by miR-139-5p promotes proliferation of clear cell renal cell carcinoma via inhibition of LATS2 activation.

Applications

Unspecified application

Species

Unspecified reactive species

Haisheng Yi,Lingyun Liu,Jingshun Zhang,Kaimin Guo,Yin Cao,Penghao Sun,Hongliang Wang

Advanced healthcare materials 12:e2301316 PubMed37531238

2023

An H S-BMP6 Dual-Loading System with Regulating Yap/Taz and Jun Pathway for Synergistic Critical Limb Ischemia Salvaging Therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Jiateng Hu,Zhijue Xu,Donghui Liao,Yihong Jiang,Hongji Pu,Zhaoyu Wu,Xintong Xu,Zhen Zhao,Jianqiang Liu,Xinwu Lu,Xiaobing Liu,Bo Li

Evidence-based complementary and alternative medicine : eCAM 2022:3945446 PubMed35280504

2022

FAM117A Is a New Prognostic Marker of Lung Adenocarcinoma and Predicts Sensitivity to PD0332991.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Wu,Jiajin Zhang,Kuan Wang,Mengjiao Fan,Yi Hu
View all publications
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Product promise

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