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AB193499

Anti-TB Ag85A antibody

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(2 Publications)

Rabbit Polyclonal TB Ag85A antibody. Suitable for WB, ELISA and reacts with Recombinant fragment - Mycobacterium tuberculosis samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Mycobacterium tuberculosis CDC1551 fbpA aa 50 to C-terminus.

View Alternative Names

mpt44, MT3911, fbpA, Diacylglycerol acyltransferase/mycolyltransferase Ag85A, DGAT, Acyl-CoA:diacylglycerol acyltransferase, Antigen 85 complex A, Fibronectin-binding protein A, 85A, Ag85A, Fbps A

1 Images
Western blot - Anti-TB Ag85A antibody (AB193499)
  • WB

Supplier Data

Western blot - Anti-TB Ag85A antibody (AB193499)

All lanes:

Western blot - Anti-TB Ag85A antibody (ab193499) at 2 µg/mL

Lane 1:

Recombinant Mycobacterium tuberculosis TB Ag85A protein at 1 µg

Lane 2:

Recombinant Mycobacterium tuberculosis TB Ag85A protein at 10 µg

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/10000 dilution

Predicted band size: 36 kDa

Observed band size: 36 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mycobacterium tuberculosis

Applications

ELISA, WB

applications

Immunogen

Recombinant Fragment Protein within Mycobacterium tuberculosis CDC1551 fbpA aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P9WQP2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mycobacterium tuberculosis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "" }, "Recombinant fragment - Mycobacterium tuberculosis": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
>95%,Protein G purified
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TB Ag85A also known as Antigen 85A is an important protein in Mycobacterium tuberculosis. It has a molecular mass of approximately 30-32 kDa. TB Ag85A is part of the antigen 85 complex which consists of proteins Ag85A Ag85B and Ag85C. This protein actively participates in mycobacterial cell wall synthesis. It catalyzes the transfer of mycolic acids to the cell wall arabinogalactan contributing to the bacteria's protective barrier. Expression occurs heavily in the cell envelope of Mycobacterium tuberculosis enabling vital interactions with the host's immune cells.
Biological function summary

TB Ag85A plays a significant role in the survival and virulence of Mycobacterium tuberculosis within host macrophages. It is part of the antigen 85 complex an important element in the synthesis of the mycobacterial cell envelope. This complex facilitates the interconversion of trehalose monomycolate and trehalose dimycolate critical for granuloma formation. The antigen 85 complex interferes with the host's immune response aiding the persistence of the bacterium. The synthesis and secretion of TB Ag85A by Mycobacterium tuberculosis allow it to adjust and survive hostile intra-host environments.

Pathways

TB Ag85A participates in the lipid biosynthesis pathway integral to cell envelope formation. This pathway plays a fundamental role in maintaining mycobacterial cell wall integrity. TB Ag85A is linked with proteins like Ag85B and Ag85C which are components of the same antigen 85 complex. These proteins work together within the pathway contributing to the robust defense system of Mycobacterium tuberculosis which complicates treatment and eradication efforts.

TB Ag85A is closely connected to tuberculosis a severe infectious disease. The protein's role in cell wall synthesis and immune evasion links it to the pathogen's ability to persist in the host. TB Ag85A's interactions with host immune defenses allow Mycobacterium tuberculosis to survive and proliferate in the lungs. Additionally studies propose TB Ag85A as a potential target for developing vaccines against tuberculosis given its role in infection and immune evasion. Researchers are exploring its connections to Ag85B and Ag85C for novel therapeutic interventions in tuberculosis management.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria for fibronectin, a large adhesive glycoprotein, which facilitates the attachment of M.tuberculosis to murine alveolar macrophages (AMs). They also help to maintain the integrity of the cell wall by catalyzing the transfer of mycolic acids to cell wall arabinogalactan, and through the synthesis of alpha,alpha-trehalose dimycolate (TDM, cord factor). They catalyze the transfer of a mycoloyl residue from one molecule of alpha,alpha-trehalose monomycolate (TMM) to another TMM, leading to the formation of TDM. FbpA mediates triacylglycerol (TAG) formation with long-chain acyl-CoA as the acyl donor and 1,2-dipalmitoyl-sn-glycerol (1,2-dipalmitin) as the acyl acceptor (By similarity).
See full target information fbpA

Additional targets

TB Ag85A
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

BMC infectious diseases 25:65 PubMed39815174

2025

Identifying plasma exosome antigens as a potential diagnostic biomarker for tuberculosis disease.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Jiang,Li Yan,Baoru Zhou,Jianming Hong,Huixing Tan,Xiaonan Li,Yong Wang

Biotechnology letters 39:1159-1166 PubMed28470626

2017

Novel adjuvant for immunization against tuberculosis: DNA vaccine expressing Mycobacterium tuberculosis antigen 85A and interleukin-15 fusion product elicits strong immune responses in mice.

Applications

WB

Species

Mouse

Li Sun,Quan Yuan,Tianhua Xu,Li Yao,Jiangmin Feng,Jianfei Ma,Lining Wang,Changlong Lv,Danan Wang
View all publications
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