Rabbit Polyclonal TBC1D15 antibody. Suitable for IHC-P, ICC/IF, WB and reacts with Human, Mouse, Rat samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TBC1D15 aa 150-300.
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- Acta pharmaceutica Sinica. B 13:4823-48392023TBC1D15 deficiency protects against doxorubicin cardiotoxicity inhibiting DNA-PKcs cytosolic retention and DNA damage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenjun Yu,Haixia Xu,Zhe Sun,Yuxin Du,Shiqun Sun,Miyesaier Abudureyimu,Mengjiao Zhang,Jun Tao,Junbo Ge,Jun Ren,Yingmei ZhangPubMed 38045047
- The EMBO journal 39:e1023012020SKIP-HOPS recruits TBC1D15 for a Rab7-to-Arl8b identity switch to control late endosome transport.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarlieke Lm Jongsma et. al.PubMed 32080880
- Nature communications 10:20592019STX17 dynamically regulated by Fis1 induces mitophagy via hierarchical macroautophagic mechanism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongxu Xian et. al.PubMed 31053718
- Gene 683:210-2152018TBC1D15 affects glucose uptake by regulating GLUT4 translocation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJia Wu,Dandan Cheng,Li Liu,Zhengbing Lv,Kuancheng LiuPubMed 30316925
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human TBC1D15 aa 150-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q8TC07
Reactivity data
Select an application| IHC-P | ICC/IF | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Expected | Tested |
| Rat | Expected | Expected | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20.00000 - 1/50.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Species Rat | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Associated Products
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Target data
Function
Acts as a GTPase activating protein for RAB7A. Does not act on RAB4, RAB5 or RAB6 (By similarity).
Alternative names
TBC1 domain family member 15, GTPase-activating protein RAB7, GAP for RAB7, Rab7-GAP, TBC1D15
Recommended products
Rabbit Polyclonal TBC1D15 antibody. Suitable for IHC-P, ICC/IF, WB and reacts with Human, Mouse, Rat samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TBC1D15 aa 150-300.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human TBC1D15 aa 150-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q8TC07
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TBC1D15 also known as TBC1 Domain Family Member 15 is a Rab GTPase-activating protein (Rab-GAP) with a molecular mass of approximately 72 kDa. This protein predominantly functions to regulate intracellular membrane trafficking by inactivating Rab GTPases such as Rab7 and Rab36. These Rab GTPases play essential roles in various cellular processes including endocytosis and vesicular trafficking. TBC1D15 is widely expressed in various tissues but demonstrated higher levels in the brain and skeletal muscle indicating tissue-specific roles or regulation.
- Biological function summary
TBC1D15 contributes to mitochondrial dynamics and autophagy processes. It functions as part of a complex with the fission protein Fis1 on the mitochondrial outer membrane. By binding to Fis1 TBC1D15 plays an important role in mediating mitochondrial fission. This activity is interconnected with cellular energy homeostasis and quality control reflecting its influence on cellular health and survival. In the context of autophagy TBC1D15 regulation appears to ensure proper cargo selection and vesicle trafficking efficiency essential steps in cellular waste management.
- Pathways
TBC1D15 participates in the mitophagy pathway and endocytic recycling pathway. The mitophagy pathway involves the removal of damaged mitochondrian important for cellular homeostasis and metabolism where TBC1D15 interacts with proteins such as Fis1 and Drp1 to facilitate mitochondrial division. In the endocytic recycling pathway TBC1D15 associates with Rab7 an important player in lysosome function and autophagy affecting cargo sorting and membrane turnover which are necessary for maintaining cellular balance and adapting to stress.
- Associated diseases and disorders
Changes in TBC1D15 activity have been linked to neurodegenerative conditions including Parkinson's disease and Alzheimer's disease. These diseases often feature mitochondrial dysfunction and defective autophagy processes in which TBC1D15 plays a significant role. Aberrations in its interaction with proteins like Rab7 and Fis1 may contribute to the pathological environment seen in these diseases emphasizing its potential as a therapeutic target or biomarker for mitochondrial-related pathologies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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8 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBC1D15 antibody (ab121396)
Immunohistochemical analysis of paraffin embedded human kidney tissue labeling TBC1D15 with ab121396 antibody at a 1/20 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-TBC1D15 antibody (ab121396)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoplasm and mitochondria. Recommended concentration of ab121396 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Western blot - Anti-TBC1D15 antibody (ab121396)
All lanes: Western blot - Anti-TBC1D15 antibody (ab121396) at 0.4 µg/mL
Lane 1: NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
Lane 2: NBT-II (rat urinary bladder tumor cell line) cell lysate
Predicted band size: 79 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBC1D15 antibody (ab121396)
Immunohistochemical analysis of paraffin embedded human testis tissue labeling TBC1D15 with ab121396 antibody at a 1/20 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBC1D15 antibody (ab121396)
Immunohistochemical analysis of paraffin embedded human placenta tissue labeling TBC1D15 with ab121396 antibody at a 1/20 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBC1D15 antibody (ab121396)
Immunohistochemical analysis of paraffin embedded human skin tissue labeling TBC1D15 with ab121396 antibody at a 1/20 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-TBC1D15 antibody (ab121396)
All lanes: Western blot - Anti-TBC1D15 antibody (ab121396) at 0.4 µg/mL
All lanes: NTERA-2 (human malignant pluripotent embryonic carcinoma cell line) cell lysate
Predicted band size: 79 kDa
Western blot - Anti-TBC1D15 antibody (ab121396)
Western blot: Rabbit Polyclonal to TBC1D15 ab121396 staining at 0.1 µg/mL, shown in green; Mouse anti GAPDH (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 75 kDa in Wild-type A549 ab288558 cell lysates with no signal observed at this size in TBC1D15 knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.
All lanes: Western blot - Anti-TBC1D15 antibody (ab121396) at 0.1 µg/mL
Lane 1: Wild-type A549 ab288558 at 20 µg
Lane 2: TBC1D15 knockout A549 at 20 µg
Lane 3: HeLa at 20 µg
Lane 4: A549 Nuclear at 20 µg
SecondaryAll lanes: Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 79 kDa
Observed band size: 75 kDa
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