Anti-TBC1D15 antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBC1D15 antibody (AB121396)

Immunohistochemical analysis of paraffin embedded human skin tissue labeling TBC1D15 with ab121396 antibody at a 1/20 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBC1D15 antibody (AB121396)

Immunohistochemical analysis of paraffin embedded human placenta tissue labeling TBC1D15 with ab121396 antibody at a 1/20 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBC1D15 antibody (AB121396)

Immunohistochemical analysis of paraffin embedded human kidney tissue labeling TBC1D15 with ab121396 antibody at a 1/20 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TBC1D15 antibody (AB121396)

Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoplasm and mitochondria. Recommended concentration of ab121396 1-4 µg/ml. Cells treated with PFA/Triton X-100.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBC1D15 antibody (AB121396)

Immunohistochemical analysis of paraffin embedded human testis tissue labeling TBC1D15 with ab121396 antibody at a 1/20 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• WB

Lab

Western blot - Anti-TBC1D15 antibody (AB121396)

Western blot : Rabbit Polyclonal to TBC1D15 ab121396 staining at 0.1 µg/mL, shown in green; Mouse anti GAPDH (ab8245) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 75 kDa in Wild-type A549 ab288558 cell lysates with no signal observed at this size in TBC1D15 knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-TBC1D15 antibody (ab121396) at 0.1 µg/mL

Lane 1:

Wild-type A549 ab288558 at 20 µg

Lane 2:

TBC1D15 knockout A549 at 20 µg

Lane 3:

HeLa at 20 µg

Lane 4:

A549 Nuclear at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 79 kDa

Observed band size: 75 kDa

false

• WB

Supplier Data

Western blot - Anti-TBC1D15 antibody (AB121396)

All lanes:

Western blot - Anti-TBC1D15 antibody (ab121396) at 0.4 µg/mL

All lanes:

NTERA-2 (human malignant pluripotent embryonic carcinoma cell line) cell lysate

Predicted band size: 79 kDa

false

• WB

Supplier Data

Western blot - Anti-TBC1D15 antibody (AB121396)

All lanes:

Western blot - Anti-TBC1D15 antibody (ab121396) at 0.4 µg/mL

Lane 1:

NIH/3T3 (mouse embryo fibroblast cell line) cell lysate

Lane 2:

NBT-II (rat urinary bladder tumor cell line) cell lysate

Predicted band size: 79 kDa

false