AB194953

Anti-TBC1D15 antibody - N-terminal

Name: Anti-TBC1D15 antibody - N-terminal (ab194953) | Abcam
Brand: Abcam Limited
SKU: AB194953
Price: 525 USD
Availability: InStock

Be the first to review this product! Submit a review

(2 Publications)

Rabbit Polyclonal TBC1D15 antibody. N-terminal. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human TBC1D15 aa 1-50.

View Alternative Names

TBC1 domain family member 15, GTPase-activating protein RAB7, GAP for RAB7, Rab7-GAP, TBC1D15

3 Images

Supplier Data

Immunoprecipitation - Anti-TBC1D15 antibody - N-terminal (AB194953)

Detection of TBC1D15 in immunoprecipitates of 293T whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) using ab194953 at 6 μg/mg lysate for IP and at 0.4 μg/ml for subsequent Western blot detection.
Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-TBC1D15 antibody - N-terminal (ab194953)

Predicted band size: 79 kDa

false

Supplier Data

Western blot - Anti-TBC1D15 antibody - N-terminal (AB194953)

Whole cell lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-TBC1D15 antibody - N-terminal (ab194953) at 0.4 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Lane 4:

TCMK-1 whole cell lysate at 50 µg

Lane 5:

NIH/3T3 whole cell lysate at 50 µg

Predicted band size: 79 kDa

true

Exposure time: 30s

Lab

Western blot - Anti-TBC1D15 antibody - N-terminal (AB194953)

Western blot : Rabbit Polyclonal to TBC1D15 ab194953 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH (ab8245) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 75 kDa in Wild-type A549 ab288558 cell lysates with no signal observed at this size in TBC1D15 knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-TBC1D15 antibody - N-terminal (ab194953) at 1/1000 dilution

Lane 1:

Wild-type A549 ab288558 at 20 µg

Lane 2:

TBC1D15 knockout A549 at 20 µg

Lane 3:

HeLa at 20 µg

Lane 4:

A549 Nuclear at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 79 kDa

Observed band size: 75 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human TBC1D15 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q8TC07

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "" }, "Cat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Common marmoset": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab194953 was affinity purified using an epitope specific to TBC1D15 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TBC1D15 also known as TBC1 Domain Family Member 15 is a Rab GTPase-activating protein (Rab-GAP) with a molecular mass of approximately 72 kDa. This protein predominantly functions to regulate intracellular membrane trafficking by inactivating Rab GTPases such as Rab7 and Rab36. These Rab GTPases play essential roles in various cellular processes including endocytosis and vesicular trafficking. TBC1D15 is widely expressed in various tissues but demonstrated higher levels in the brain and skeletal muscle indicating tissue-specific roles or regulation.

Biological function summary

TBC1D15 contributes to mitochondrial dynamics and autophagy processes. It functions as part of a complex with the fission protein Fis1 on the mitochondrial outer membrane. By binding to Fis1 TBC1D15 plays an important role in mediating mitochondrial fission. This activity is interconnected with cellular energy homeostasis and quality control reflecting its influence on cellular health and survival. In the context of autophagy TBC1D15 regulation appears to ensure proper cargo selection and vesicle trafficking efficiency essential steps in cellular waste management.

Pathways

TBC1D15 participates in the mitophagy pathway and endocytic recycling pathway. The mitophagy pathway involves the removal of damaged mitochondrian important for cellular homeostasis and metabolism where TBC1D15 interacts with proteins such as Fis1 and Drp1 to facilitate mitochondrial division. In the endocytic recycling pathway TBC1D15 associates with Rab7 an important player in lysosome function and autophagy affecting cargo sorting and membrane turnover which are necessary for maintaining cellular balance and adapting to stress.

Changes in TBC1D15 activity have been linked to neurodegenerative conditions including Parkinson's disease and Alzheimer's disease. These diseases often feature mitochondrial dysfunction and defective autophagy processes in which TBC1D15 plays a significant role. Aberrations in its interaction with proteins like Rab7 and Fis1 may contribute to the pathological environment seen in these diseases emphasizing its potential as a therapeutic target or biomarker for mitochondrial-related pathologies.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Acts as a GTPase activating protein for RAB7A. Does not act on RAB4, RAB5 or RAB6 (By similarity).

See full target information TBC1D15

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 8:593659 PubMed33330477

2020

Echinacoside Suppresses Amyloidogenesis and Modulates F-actin Remodeling by Targeting the ER Stress Sensor PERK in a Mouse Model of Alzheimer's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Dai,Guanghui Han,Shijun Xu,Yongna Yuan,Chunyan Zhao,Tao Ma

Cell death and differentiation 28:1333-1346 PubMed33168956

2020

Targeting TOPK sensitises tumour cells to radiation-induced damage by enhancing replication stress.

Applications

Unspecified application

Species

Unspecified reactive species

Katharine J Herbert,Rathi Puliyadi,Remko Prevo,Gonzalo Rodriguez-Berriguete,Anderson Ryan,Kristijan Ramadan,Geoff S Higgins

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TBC1D15 antibody - N-terminal

Immunoprecipitation - Anti-TBC1D15 antibody - N-terminal (AB194953)

Western blot - Anti-TBC1D15 antibody - N-terminal (AB194953)

Western blot - Anti-TBC1D15 antibody - N-terminal (AB194953)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise