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AB176701

Anti-TBC1D2 antibody - N-terminal

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(1 Publication)

Rabbit Polyclonal TBC1D2 antibody. N-terminal. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human TBC1D2 aa 1-50.

View Alternative Names

PARIS1, PP8997, TBC1D2A, TBC1D2, TBC1 domain family member 2A, Armus, Prostate antigen recognized and identified by SEREX 1, PARIS-1

2 Images
Immunoprecipitation - Anti-TBC1D2 antibody - N-terminal (AB176701)
  • IP

Supplier Data

Immunoprecipitation - Anti-TBC1D2 antibody - N-terminal (AB176701)

Detection of TBC1D2 by Western Blot of Immunprecipitate.

Western blot using ab176701 at 0.4 μg/ml staining TBC1D2 in HeLa whole cell lysate immunoprecipitated using ab176701 at 6 μg/mg lysate (1 mg/IP; 20% of IP loaded/lane).

Detection : Chemiluminescence with exposure times of 3 seconds

All lanes:

Immunoprecipitation - Anti-TBC1D2 antibody - N-terminal (ab176701)

Predicted band size: 105 kDa

false

Western blot - Anti-TBC1D2 antibody - N-terminal (AB176701)
  • WB

Supplier Data

Western blot - Anti-TBC1D2 antibody - N-terminal (AB176701)

All lanes:

Western blot - Anti-TBC1D2 antibody - N-terminal (ab176701) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

293T whole cell lysate at 50 µg

Lane 4:

Jurkat whole cell lysate at 50 µg

Predicted band size: 105 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human TBC1D2 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q9BYX2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: 99% Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TBC1D2 also known as Armus is a protein involved in tethering and trafficking of endosomes exhibiting a molecular mass of approximately 120 kDa. It interacts with Rab7 a small GTPase to regulate the elongation of endosomal structures. Expression of TBC1D2 is found in many tissues with significant levels in the brain and adipose tissue. The protein functions as a GAP (GTPase-activating protein) facilitating the conversion of Rab7-GTP to Rab7-GDP which is an important process in endosomal transport and maturation.
Biological function summary

TBC1D2 plays a role in the regulation of cellular autophagy and endocytosis. It is part of the endosomal complex involved in cargo sorting and trafficking affecting the lysosomal degradation pathway. The interaction with Rab7 suggests its involvement in the fusion of late endosomes with lysosomes. Additionally TBC1D2 regulates actin cytoskeleton dynamics by modulating signal transduction pathways impacting various cellular processes such as cell migration and morphogenesis.

Pathways

TBC1D2 participates in the endocytic and autophagic pathways influencing cellular homeostasis and response to stress. In the endocytic pathway it works closely with proteins like Rab7 to modulate endosome maturation. TBC1D2 also intersects with other autophagic-related proteins impacting cellular recycling processes important for maintaining cellular environment stability and responding to nutrient availability.

TBC1D2 is linked to neurodegenerative and metabolic conditions. Aberrant regulation of its activity is associated with Alzheimer’s disease affecting amyloid precursor protein processing and clearance. Moreover TBC1D2's involvement in adipose tissue function connects it to obesity-related disorders influencing body weight and metabolism. Its interactions with proteins like Rab7 in these disease contexts underline its critical role in cellular trafficking and metabolic regulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a GTPase-activating protein for RAB7A. Signal effector acting as a linker between RAC1 and RAB7A, leading to RAB7A inactivation and subsequent inhibition of cadherin degradation and reduced cell-cell adhesion.
See full target information TBC1D2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Archives of medical science : AMS 16:1394-1401 PubMed33224339

2019

MiRNA-610 acts as a tumour suppressor to depress the cisplatin resistance in hepatocellular carcinoma through targeted silencing of hepatoma-derived growth factor.

Applications

Unspecified application

Species

Unspecified reactive species

Yongqing Xu,Helin Wang,Weike Gao
View all publications
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Product promise

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