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AB237685

Anti-TBC1D20 antibody

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(1 Publication)

Rabbit Polyclonal TBC1D20 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human TBC1D20 aa 1-250.

View Alternative Names

C20orf140, TBC1D20, TBC1 domain family member 20

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBC1D20 antibody (AB237685)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBC1D20 antibody (AB237685)

Paraffin-embedded human testis tissue stained for TBC1D20 using ab237685 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-TBC1D20 antibody (AB237685)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TBC1D20 antibody (AB237685)

MCF7 (human breast adenocarcinoma cell line) cells stained for TBC1D20 (green) using ab237685 at 1/100 dilution in ICC/IF. Secondary antibody is Alexa Fluor® 488-congugated Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBC1D20 antibody (AB237685)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBC1D20 antibody (AB237685)

Paraffin-embedded human adrenal gland tissue stained for TBC1D20 using ab237685 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-TBC1D20 antibody (AB237685)
  • WB

Characteriser

Western blot - Anti-TBC1D20 antibody (AB237685)

All lanes:

Western blot - Anti-TBC1D20 antibody (ab237685) at 1/500 dilution

Lane 1:

Mouse brain lysate

Lane 2:

Mouse stomach lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 46 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human TBC1D20 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

Q96BZ9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity greater than 95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TBC1D20 also known as TBC domain family member 20 is a Rab GTPase-activating protein with an approximate mass of 45 kDa. This protein is primarily expressed in the endoplasmic reticulum and is involved in the regulation of Rab GTPases which are critical for intracellular membrane trafficking. TBC1D20 interacts specifically with Rab1 and Rab2 playing a role in the control of vesicle formation trafficking and fusion processes.
Biological function summary

TBC1D20 influences cellular functions by acting as a regulator of autophagy and endocytosis processes. It participates in the modulation of endoplasmic reticulum morphology influencing its shape and function. TBC1D20 does not typically form part of any larger complex but acts through its interactions with Rab proteins and other associated proteins involved in vesicle transport. Its activity is vital for maintaining proper cellular homeostasis by ensuring efficient cargo delivery.

Pathways

TBC1D20 plays a part in the endocytic pathway and autophagic processes important for cellular nutrient regulation and waste management. It interacts with proteins such as LC3 which are instrumental in autophagosome formation. TBC1D20 ensures the timely deactivation of Rab proteins coordinating with other GTPase-activating proteins to enforce proper vesicular trafficking along these pathways.

TBC1D20's dysfunction is linked to conditions like Charcot-Marie-Tooth disease and some neurodegenerative disorders. The protein's role in membrane trafficking and autophagy ties it to the etiology of these diseases with disruptions in these pathways leading to pathological manifestations. TBC1D20 is also connected to proteins such as MFN2 which is involved in mitochondrial dynamics highlighting its broader impact on cellular function through pathway disruption.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

GTPase-activating protein specific for Rab1 and Rab2 small GTPase families for which it can accelerate the intrinsic GTP hydrolysis rate by more than five orders of magnitude (PubMed : 23236136). Involved in maintaining endoplasmic reticulum structure (PubMed : 24891604).
See full target information TBC1D20
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer medicine 12:4968-4980 PubMed36056909

2022

Risk stratification and prognosis prediction based on inflammation-related gene signature in lung squamous carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wenyu Zhai,Si Chen,Fangfang Duan,Junye Wang,Zerui Zhao,Yaobin Lin,Bingyu Rao,Yizhi Wang,Lie Zheng,Hao Long
View all publications
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Product promise

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