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AB185102

Anti-TBC1D2B antibody

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(2 Publications)

Rabbit Polyclonal TBC1D2B antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TBC1D2B aa 250-400.

View Alternative Names

KIAA1055, TBC1D2B, TBC1 domain family member 2B

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBC1D2B antibody (AB185102)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBC1D2B antibody (AB185102)

Immunohistochemical analysis of paraffin-embedded Human hippocampus tissue, labeling TBC1D2B using ab185102 at a 1/50 dilution.

Western blot - Anti-TBC1D2B antibody (AB185102)
  • WB

Supplier Data

Western blot - Anti-TBC1D2B antibody (AB185102)

All lanes:

Western blot - Anti-TBC1D2B antibody (ab185102) at 1/100 dilution

Lane 1:

RT-4 cell lysate

Lane 2:

U-251 MG sp cell lysate

Predicted band size: 110 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human TBC1D2B aa 250-400. The exact immunogen used to generate this antibody is proprietary information.

Q9UPU7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p>Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TBC1D2B also known as TBC domain family member 2B is a Rab GTPase-activating protein (GAP) that plays an important role in regulating intracellular trafficking. The protein specifically accelerates the GTPase activity of Rab proteins which are key regulators of vesicle transport. TBC1D2B has a molecular mass of approximately 79 kDa and is widely expressed in various tissues including the brain liver and kidney.
Biological function summary

The TBC1D2B protein modulates vesicular traffic by acting as a GAP for Rab proteins specifically interacting with Rab7 a member of the Ras superfamily involved in endo-lysosomal trafficking. TBC1D2B acts as a negative regulator ensuring that the correct timing and specificity of vesicle fusion events are maintained. It functions as part of a complex with other proteins that together modulate endosome dynamics.

Pathways

TBC1D2B plays a significant role in the endocytosis pathway and the lysosome pathway. It interacts closely with the Rab7 protein where it controls vesicle movement and fusion between endosomes and lysosomes. Its regulatory activity on Rab7 and consequently on lysosomal function impacts the efficiency of the degradation pathway ensuring cellular homeostasis is maintained.

Mutations or dysregulation of TBC1D2B have been linked to neurodegenerative diseases and cancer. Defects in Rab7 regulation can disrupt endo-lysosomal pathways contributing to the development of pathologies in conditions such as Charcot-Marie-Tooth disease and potentially various cancers where cellular recycling processes are altered. Understanding TBC1D2B's interactions with Rab7 offers insight into the mechanisms underlying these diseases and provides a potential target for therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

GTPase-activating protein that plays a role in the early steps of endocytosis (PubMed : 32623794).
See full target information TBC1D2B
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of extracellular biology 3:e141 PubMed38939899

2024

RAB31 drives extracellular vesicle fusion and cancer-associated fibroblast formation leading to oxaliplatin resistance in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Chan Chang,Yi-Fang Yang,Chien-Hsiu Li,Ming-Hsien Chan,Meng-Lun Lu,Ming-Huang Chen,Chi-Long Chen,Michael Hsiao

International journal of molecular sciences 24: PubMed37895057

2023

Ubiquitin Proteasome System Role in Diabetes-Induced Cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Ortal Nahum-Ankonina,Efrat Kurtzwald-Josefson,Aaron Ciechanover,Maayan Waldman,Orna Shwartz-Rohaker,Edith Hochhauser,Sam J Meyer,Dan Aravot,Moshe Phillip,Yaron D Barac
View all publications
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Product promise

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