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AB203896

Anti-TBC1D5 antibody

5

(3 Reviews)

|

(3 Publications)

Rabbit Polyclonal TBC1D5 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TBC1D5 aa 500-650.

View Alternative Names

KIAA0210, TBC1D5, TBC1 domain family member 5

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBC1D5 antibody (AB203896)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBC1D5 antibody (AB203896)

Immunohistochemical analysis of human fallopian tube tissue labeling TBC1D5 beta with ab203896 at 1/1000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBC1D5 antibody (AB203896)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBC1D5 antibody (AB203896)

Immunohistochemical analysis of human testis tissue labeling TBC1D5 beta with ab203896 at 1/1000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBC1D5 antibody (AB203896)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBC1D5 antibody (AB203896)

Immunohistochemical analysis of human lymph node tissue labeling TBC1D5 beta with ab203896 at 1/1000 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-TBC1D5 antibody (AB203896)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TBC1D5 antibody (AB203896)

Immunocytochemical analysis of A431 cells labeling TBC1D5 beta with ab203896 at 1 μg/mL.

Western blot - Anti-TBC1D5 antibody (AB203896)
  • WB

Supplier Data

Western blot - Anti-TBC1D5 antibody (AB203896)

All lanes:

Western blot - Anti-TBC1D5 antibody (ab203896)

Lane 1:

RT-4 cell lysate at 0.4 µg/mL

Lane 2:

U-251 cell lysate at 0.4 µg/mL

Predicted band size: 89 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human TBC1D5 aa 500-650. The exact immunogen used to generate this antibody is proprietary information.

Q92609

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The TBC1D5 protein also known as TBC1 domain family member 5 is involved in intracellular trafficking. It acts as a GTPase-activating protein (GAP) that regulates Rab GTPases. The molecular weight of TBC1D5 is approximately 76 kDa. This protein is widely expressed in various tissues with higher levels in neural tissues. Its presence suggests a role in cellular processes that require precise regulation of membrane trafficking and recycling dynamics.
Biological function summary

TBC1D5 regulates endosomal dynamics and is part of the retromer complex. It interacts with endosomal membranes helping to control the sorting and recycling of cargo proteins. The TBC1D5 protein modulates Rab7 and Rab9 activity by promoting GTP hydrolysis which influences vesicular trafficking. By coordinating with other retromer subunits like VPS29 and VPS35 TBC1D5 plays a significant role in maintaining cellular processes that require effective cargo transport and endosome maturation.

Pathways

TBC1D5 is closely linked to the endosomal trafficking and retrograde transport pathways. It connects functionally within the context of these pathways by interacting with Rab GTPases such as Rab7 and Rab9. TBC1D5 ensures proper endosome-to-Golgi transport modulating the dissociation and recycling of the retromer complex. Its actions influence how cargo is sorted and directed back to the trans-Golgi network impacting the downstream signaling events required for cellular homeostasis.

TBC1D5 has associations with neurodegenerative conditions like Parkinson's disease. In Parkinson's retromer dysfunction and disrupted endosomal sorting are observed implicating TBC1D5 in these pathological processes. Abnormalities in TBC1D5 function may lead to improper cargo sorting contributing to disease pathogenesis. The protein's relationship with VPS26 another retromer component plays a role in neurodegenerative disorder mechanisms highlighting a complex network affecting neuron survival and health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May act as a GTPase-activating protein (GAP) for Rab family protein(s). May act as a GAP for RAB7A. Can displace RAB7A and retromer CSC subcomplex from the endosomal membrane to the cytosol; at least retromer displacement seems to require its catalytic activity (PubMed : 19531583, PubMed : 20923837). Required for retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN); the function seems to require its catalytic activity. Involved in regulation of autophagy (PubMed : 22354992). May act as a molecular switch between endosomal and autophagosomal transport and is involved in reprogramming vesicle trafficking upon autophagy induction. Involved in the trafficking of ATG9A upon activation of autophagy. May regulate the recruitment of ATG9A-AP2-containing vesicles to autophagic membranes (PubMed : 24603492).
See full target information TBC1D5
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cancer immunology, immunotherapy : CII 73:96 PubMed38619621

2024

CD200 is overexpressed in the pancreatic tumor microenvironment and predictive of overall survival.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica Wedig,Shrina Jasani,Debasmita Mukherjee,Hannah Lathrop,Priya Matreja,Timothy Pfau,Liliana D'Alesio,Abigail Guenther,Lexie Fenn,Morgan Kaiser,Molly A Torok,Jake McGue,Gina M Sizemore,Anne M Noonan,Mary E Dillhoff,Bradley W Blaser,Timothy L Frankel,Stacey Culp,Phil A Hart,Zobeida Cruz-Monserrate,Thomas A Mace

PLoS pathogens 19:e1011648 PubMed37703297

2023

Noncanonical Rab9a action supports retromer-mediated endosomal exit of human papillomavirus during virus entry.

Applications

Unspecified application

Species

Unspecified reactive species

Jeongjoon Choi,Daniel DiMaio

Cell reports 31:107750 PubMed32521275

2020

TBC1D5-Catalyzed Cycling of Rab7 Is Required for Retromer-Mediated Human Papillomavirus Trafficking during Virus Entry.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Xie,Erin N Heim,Mac Crite,Daniel DiMaio
View all publications
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