Rabbit Polyclonal TBC1D5 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TBC1D5 aa 500-650.
Images
Publications
Filter by
- PLoS pathogens 19:e10116482023Noncanonical Rab9a action supports retromer-mediated endosomal exit of human papillomavirus during virus entry.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJeongjoon Choi,Daniel DiMaioPubMed 37703297
- Cell reports 31:1077502020TBC1D5-Catalyzed Cycling of Rab7 Is Required for Retromer-Mediated Human Papillomavirus Trafficking during Virus Entry.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJian Xie,Erin N Heim,Mac Crite,Daniel DiMaioPubMed 32521275
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human TBC1D5 aa 500-650. The exact immunogen used to generate this antibody is proprietary information. Database link Q92609
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
May act as a GTPase-activating protein (GAP) for Rab family protein(s). May act as a GAP for RAB7A. Can displace RAB7A and retromer CSC subcomplex from the endosomal membrane to the cytosol; at least retromer displacement seems to require its catalytic activity (PubMed:19531583, PubMed:20923837). Required for retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN); the function seems to require its catalytic activity. Involved in regulation of autophagy (PubMed:22354992). May act as a molecular switch between endosomal and autophagosomal transport and is involved in reprogramming vesicle trafficking upon autophagy induction. Involved in the trafficking of ATG9A upon activation of autophagy. May regulate the recruitment of ATG9A-AP2-containing vesicles to autophagic membranes (PubMed:24603492).
Alternative names
KIAA0210, TBC1D5, TBC1 domain family member 5
Recommended products
Rabbit Polyclonal TBC1D5 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TBC1D5 aa 500-650.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human TBC1D5 aa 500-650. The exact immunogen used to generate this antibody is proprietary information. Database link Q92609
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The TBC1D5 protein also known as TBC1 domain family member 5 is involved in intracellular trafficking. It acts as a GTPase-activating protein (GAP) that regulates Rab GTPases. The molecular weight of TBC1D5 is approximately 76 kDa. This protein is widely expressed in various tissues with higher levels in neural tissues. Its presence suggests a role in cellular processes that require precise regulation of membrane trafficking and recycling dynamics.
- Biological function summary
TBC1D5 regulates endosomal dynamics and is part of the retromer complex. It interacts with endosomal membranes helping to control the sorting and recycling of cargo proteins. The TBC1D5 protein modulates Rab7 and Rab9 activity by promoting GTP hydrolysis which influences vesicular trafficking. By coordinating with other retromer subunits like VPS29 and VPS35 TBC1D5 plays a significant role in maintaining cellular processes that require effective cargo transport and endosome maturation.
- Pathways
TBC1D5 is closely linked to the endosomal trafficking and retrograde transport pathways. It connects functionally within the context of these pathways by interacting with Rab GTPases such as Rab7 and Rab9. TBC1D5 ensures proper endosome-to-Golgi transport modulating the dissociation and recycling of the retromer complex. Its actions influence how cargo is sorted and directed back to the trans-Golgi network impacting the downstream signaling events required for cellular homeostasis.
- Associated diseases and disorders
TBC1D5 has associations with neurodegenerative conditions like Parkinson's disease. In Parkinson's retromer dysfunction and disrupted endosomal sorting are observed implicating TBC1D5 in these pathological processes. Abnormalities in TBC1D5 function may lead to improper cargo sorting contributing to disease pathogenesis. The protein's relationship with VPS26 another retromer component plays a role in neurodegenerative disorder mechanisms highlighting a complex network affecting neuron survival and health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
Western blot - Anti-TBC1D5 antibody (ab203896)
All lanes: Western blot - Anti-TBC1D5 antibody (ab203896)
Lane 1: RT-4 cell lysate at 0.4 µg/mL
Lane 2: U-251 cell lysate at 0.4 µg/mL
Predicted band size: 89 kDa
Immunocytochemistry/ Immunofluorescence - Anti-TBC1D5 antibody (ab203896)
Immunocytochemical analysis of A431 cells labeling TBC1D5 beta with ab203896 at 1 μg/mL.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBC1D5 antibody (ab203896)
Immunohistochemical analysis of human testis tissue labeling TBC1D5 beta with ab203896 at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBC1D5 antibody (ab203896)
Immunohistochemical analysis of human fallopian tube tissue labeling TBC1D5 beta with ab203896 at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBC1D5 antibody (ab203896)
Immunohistochemical analysis of human lymph node tissue labeling TBC1D5 beta with ab203896 at 1/1000 dilution.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com