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AB308578

Anti-TBC1D5 antibody [HL2054]

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Rabbit Recombinant Monoclonal TBC1D5 antibody. Carrier free. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human TBC1D5.

View Alternative Names

KIAA0210, TBC1D5, TBC1 domain family member 5

1 Images
Western blot - Anti-TBC1D5 antibody [HL2054] (AB308578)
  • WB

Supplier Data

Western blot - Anti-TBC1D5 antibody [HL2054] (AB308578)

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TBC1D5 antibody HL2054 (ab308578) diluted at 1/1000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-TBC1D5 antibody [HL2054] (ab308578) at 1/1000 dilution

Lane 1:

Daudi cell lysate at 30 µg

Lane 2:

HeLa cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 89.004 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL2054

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human TBC1D5. The exact immunogen used to generate this antibody is proprietary information.

Q92609

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Reactivity data

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The TBC1D5 protein also known as TBC1 domain family member 5 is involved in intracellular trafficking. It acts as a GTPase-activating protein (GAP) that regulates Rab GTPases. The molecular weight of TBC1D5 is approximately 76 kDa. This protein is widely expressed in various tissues with higher levels in neural tissues. Its presence suggests a role in cellular processes that require precise regulation of membrane trafficking and recycling dynamics.
Biological function summary

TBC1D5 regulates endosomal dynamics and is part of the retromer complex. It interacts with endosomal membranes helping to control the sorting and recycling of cargo proteins. The TBC1D5 protein modulates Rab7 and Rab9 activity by promoting GTP hydrolysis which influences vesicular trafficking. By coordinating with other retromer subunits like VPS29 and VPS35 TBC1D5 plays a significant role in maintaining cellular processes that require effective cargo transport and endosome maturation.

Pathways

TBC1D5 is closely linked to the endosomal trafficking and retrograde transport pathways. It connects functionally within the context of these pathways by interacting with Rab GTPases such as Rab7 and Rab9. TBC1D5 ensures proper endosome-to-Golgi transport modulating the dissociation and recycling of the retromer complex. Its actions influence how cargo is sorted and directed back to the trans-Golgi network impacting the downstream signaling events required for cellular homeostasis.

TBC1D5 has associations with neurodegenerative conditions like Parkinson's disease. In Parkinson's retromer dysfunction and disrupted endosomal sorting are observed implicating TBC1D5 in these pathological processes. Abnormalities in TBC1D5 function may lead to improper cargo sorting contributing to disease pathogenesis. The protein's relationship with VPS26 another retromer component plays a role in neurodegenerative disorder mechanisms highlighting a complex network affecting neuron survival and health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May act as a GTPase-activating protein (GAP) for Rab family protein(s). May act as a GAP for RAB7A. Can displace RAB7A and retromer CSC subcomplex from the endosomal membrane to the cytosol; at least retromer displacement seems to require its catalytic activity (PubMed : 19531583, PubMed : 20923837). Required for retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN); the function seems to require its catalytic activity. Involved in regulation of autophagy (PubMed : 22354992). May act as a molecular switch between endosomal and autophagosomal transport and is involved in reprogramming vesicle trafficking upon autophagy induction. Involved in the trafficking of ATG9A upon activation of autophagy. May regulate the recruitment of ATG9A-AP2-containing vesicles to autophagic membranes (PubMed : 24603492).
See full target information TBC1D5
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