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AB121780

Anti-TBC1D8B antibody

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(2 Publications)

Rabbit Polyclonal TBC1D8B antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TBC1D8B aa 900 to C-terminus.

View Alternative Names

TBC1 domain family member 8B, TBC1D8B

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBC1D8B antibody (AB121780)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBC1D8B antibody (AB121780)

ab121780 at a 1/50 dilution staining TBC1D8B in Human urinary bladder tissue (paraffin embedded) by immunohistochemistry.

Immunocytochemistry/ Immunofluorescence - Anti-TBC1D8B antibody (AB121780)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TBC1D8B antibody (AB121780)

Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoplasm. Recommended concentration of ab121780 1-4 µg/ml. Cells treated with PFA/Triton X-100.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human TBC1D8B aa 900 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q0IIM8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TBC1D8B also known as TBC1 domain family member 8B acts as a GTPase-activating protein (GAP) that targets Rab GTPases. It weighs approximately 106 kDa. This protein aids in regulating endosomal trafficking processes. Human tissues show TBC1D8B expression particularly in the liver and kidney. Through its GAP activity TBC1D8B plays a role in vesicle transport systems within cells affecting how cellular materials are sorted and transported into lysosomes.
Biological function summary

TBC1D8B functions as an important regulator in cellular trafficking pathways affecting how cells manage internal transport processes. It does not exist as part of a larger known complex but independently influences endosomal sorting. By acting on Rab proteins it affects vesicular recycling and transport facilitating the correct targeting of proteins to their cellular destinations. These activities support critical cell signaling and maintenance functions at the molecular level.

Pathways

TBC1D8B plays contributory roles in the regulation of the Ras-related protein Rab pathways and the endocytosis pathway. Rab GTPases like Rab11 work in conjunction with TBC1D8B to promote efficient recycling of endosomes. This is important for maintaining membrane dynamics and cellular homeostasis. Being part of these pathways TBC1D8B ensures that cellular surfaces are properly modified to respond to environmental signals.

Researchers link TBC1D8B to focal segmental glomerulosclerosis (FSGS) and certain types of inherited kidney diseases. Mutations or malfunctions in TBC1D8B can lead to defective endosomal trafficking contributing to these conditions. Protein partners including nephrin and podocin relate to TBC1D8B in the context of FSGS emphasizing its role in maintaining kidney function and health. Understanding these connections may help in developing targeted therapies for related disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in vesicular recycling, probably as a RAB11B GTPase-activating protein.
See full target information TBC1D8B
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of the American Society of Nephrology : JA 30:2338-2353 PubMed31732614

2019

Mutations Implicate RAB11-Dependent Vesicular Trafficking in the Pathogenesis of Nephrotic Syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Lina L Kampf,Ronen Schneider,Lea Gerstner,Roland Thünauer,Mengmeng Chen,Martin Helmstädter,Ali Amar,Ana C Onuchic-Whitford,Reyner Loza Munarriz,Afig Berdeli,Dominik Müller,Eva Schrezenmeier,Klemens Budde,Shrikant Mane,Kristen M Laricchia,Heidi L Rehm,Daniel G MacArthur,Richard P Lifton,Gerd Walz,Winfried Römer,Carsten Bergmann,Friedhelm Hildebrandt,Tobias Hermle

Annals of neurology 78:568-83 PubMed26174152

2015

Isoform-specific antibodies reveal distinct subcellular localizations of C9orf72 in amyotrophic lateral sclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Shangxi Xiao,Laura MacNair,Philip McGoldrick,Paul M McKeever,Jesse R McLean,Ming Zhang,Julia Keith,Lorne Zinman,Ekaterina Rogaeva,Janice Robertson
View all publications
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Product promise

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