JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB238761

Anti-TBCD/SSD1 antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal TBCD/SSD1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human TBCD aa 1-100.

View Alternative Names

KIAA0988, SSD1, TFCD, PP1096, TBCD, Tubulin-specific chaperone D, Beta-tubulin cofactor D, SSD-1, Tubulin-folding cofactor D, tfcD

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-TBCD/SSD1 antibody (AB238761)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TBCD/SSD1 antibody (AB238761)

4% formaldehyde-fixed, 0.2% Triton X-100 permeabilized A549 (Human lung carcinoma cell line) cells stained for TBCD/SSD1 (green) using ab238761 at 1/100 dilution in ICC/IF, followed by an Alexa Fluor®488 conjugated Goat Anti-Rabbit IgG (H+L). Counter-stained with DAPI.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBCD/SSD1 antibody (AB238761)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBCD/SSD1 antibody (AB238761)

Paraffin-embedded human cervical cancer tissue stained for TBCD/SSD1 using ab238761 at 1/300 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human TBCD aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q9BTW9

style
left-column

Complementary Products

Primary Antibodies

AB240593

Anti-ETFB antibody

Immunoprecipitation - Anti-ETFB antibody (AB240593)

  • 0
  • 0
Primary Antibodies

AB73113

Anti-LRSAM1 antibody

Western blot - Anti-LRSAM1 antibody (AB73113)

  • 0
  • 0
Primary Antibodies

AB76818

Anti-IFIT3/P60 antibody

Western blot - Anti-IFIT3/P60 antibody (AB76818)

  • 0
  • 0
Primary Antibodies

AB182006

Anti-DHX33 antibody [EPR14093]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-DHX33 antibody [EPR14093] (AB182006)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity greater than 95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TBCD also known as Tubulin Folding Cofactor D or SSD1 is a critical protein for tubulin folding and microtubule dynamics. It has a molecular mass of approximately 57 kDa and is located in various tissues with higher expression in the brain and testis. The protein works in combination with other tubulin cofactors to assist in proper β-tubulin folding and function which is important for maintaining cytoskeletal integrity.
Biological function summary

TBCD operates in conjunction with other proteins in a complex playing a significant role in microtubule biogenesis. It interacts with proteins such as TUBA1B to facilitate the proper folding and stability of β-tubulin. This process is essential for cell division and intracellular transport. Additionally it impacts neuronal structure by regulating axonal transport mechanisms influencing how neurons maintain their connections and communications.

Pathways

TBCD is integral to the microtubule dynamics pathway influencing cytoskeletal organization and function. It directly interacts with the microtubule-associated proteins like TUBG1 which is important in microtubule nucleation. TBCD also participates in the pathways regulating cell cycle and mitosis linked to proteins such as TUBB affecting how cells replicate and distribute genetic material during division.

Mutations or dysregulation in TBCD have associations with neurodegenerative diseases specifically Charcot-Marie-Tooth disease. This condition affects peripheral nerves where TBCD's interaction with β-tubulin is disrupted leading to improper neuronal function. Additionally TBCD connects to KIF1A a kinesin family member involved in axonal transport contributing to the pathology of certain neurodevelopmental disorders. These links highlight TBCD's importance in maintaining neural health and function.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Tubulin-folding protein implicated in the first step of the tubulin folding pathway and required for tubulin complex assembly. Involved in the regulation of microtubule polymerization or depolymerization, it modulates microtubule dynamics by capturing GTP-bound beta-tubulin (TUBB). Its ability to interact with beta tubulin is regulated via its interaction with ARL2. Acts as a GTPase-activating protein (GAP) for ARL2. Induces microtubule disruption in absence of ARL2. Increases degradation of beta tubulin, when overexpressed in polarized cells. Promotes epithelial cell detachment, a process antagonized by ARL2. Induces tight adherens and tight junctions disassembly at the lateral cell membrane (PubMed : 10722852, PubMed : 10831612, PubMed : 11847227, PubMed : 20740604, PubMed : 27666370, PubMed : 28158450). Required for correct assembly and maintenance of the mitotic spindle, and proper progression of mitosis (PubMed : 27666370). Involved in neuron morphogenesis (PubMed : 27666374).
See full target information TBCD
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Middle East journal of digestive diseases 12:39-44 PubMed32082520

2020

Assessment of Immuno-Histochemical Expression of MBD1 in Colorectal Adenocarcinoma and Its Correlations with Prognostic Factors.

Applications

Unspecified application

Species

Unspecified reactive species

Sholeh Ghaedamini,Mehdi Nikbakht,Mitra Soleimani
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com