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AB96188

Anti-TBCK antibody

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Rabbit Polyclonal HSPC302 antibody. Suitable for WB and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human TBCK aa 350-650.

View Alternative Names

FERRY1, TBCKL, HSPC302, TBCK, TBC domain-containing protein kinase-like protein, FERRY endosomal RAB5 effector complex subunit 1, Fy-1

2 Images
Western blot - Anti-TBCK antibody (AB96188)
  • WB

Unknown

Western blot - Anti-TBCK antibody (AB96188)

7.5% SDS PAGE

All lanes:

Western blot - Anti-TBCK antibody (ab96188) at 1/1000 dilution

Lane 1:

HepG2 whole cell lysate at 30 µg

Lane 2:

Molt4 whole cell lysate at 30 µg

Predicted band size: 101 kDa

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Western blot - Anti-TBCK antibody (AB96188)
  • WB

Supplier Data

Western blot - Anti-TBCK antibody (AB96188)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-TBCK antibody (ab96188) at 1/1000 dilution

Lane 1:

NIH-3T3 whole cell extracts at 30 µg

Lane 2:

JC whole cell extracts at 30 µg

Lane 3:

BCL-1 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 101 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human TBCK aa 350-650. The exact immunogen used to generate this antibody is proprietary information.

Q8TEA7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TBCK also known as TBC1 domain-containing kinase has a molecular mass of approximately 139 kDa. It is a serine/threonine kinase that regulates protein phosphorylation important for normal cellular functions. TBCK is expressed in various tissues with significant expression levels observed in the brain and testis. This distribution hints at its functions in specialized cellular processes in these organs.
Biological function summary

TBCK influences cell growth differentiation and autophagy. It might be part of specific protein complexes involved in the mTOR signaling pathway which governs cell proliferation and survival by relaying growth signals. By interacting with other proteins TBCK plays a regulatory role in these critical cellular functions impacting overall cellular homeostasis.

Pathways

TBCK is an integral component of the mTOR and insulin signaling pathways. These pathways are central to nutrient sensing and energy balance in cells. Within these pathways TBCK interactions include phosphorylation of downstream targets such as S6K1 which is essential for protein synthesis. Through its regulatory role TBCK maintains balance and coordination with other proteins like Akt further influencing cellular metabolic responses.

Mutations or dysregulation in the TBCK gene associate with TBCK-related encephalopathy and neurodevelopmental disorders. Alterations in its normal function can disrupt mTOR signaling impacting neural function and development. TBCK is potentially linked to disorders like autism spectrum disorder with connections through proteins such as TSC1 and TSC2 which also play a part in mTOR pathway regulation. Understanding TBCK's role in these conditions offers insight into potential therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the FERRY complex (Five-subunit Endosomal Rab5 and RNA/ribosome intermediary) (PubMed : 37267905). The FERRY complex directly interacts with mRNAs and RAB5A, and functions as a RAB5A effector involved in the localization and the distribution of specific mRNAs most likely by mediating their endosomal transport. The complex recruits mRNAs and ribosomes to early endosomes through direct mRNA-interaction (PubMed : 37267905). Also involved in the modulation of mTOR signaling and expression of mTOR complex components (PubMed : 23977024, PubMed : 27040691). Involved in the control of actin-cytoskeleton organization (PubMed : 23977024).
See full target information TBCK
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Product promise

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